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A fluorescent glucose sensor

A glucose sensor and glucose technology, applied in the field of electrochemical detection, can solve the problems of low sensitivity and slow response

Active Publication Date: 2016-03-02
MEDTRUM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, various detection methods for glucose have shortcomings such as low sensitivity and slow response, which cannot meet actual needs. Therefore, it is necessary to study a detection method with high sensitivity and fast response.

Method used

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  • A fluorescent glucose sensor
  • A fluorescent glucose sensor
  • A fluorescent glucose sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Preparation of CdTe Semiconductor Quantum Dots

[0080] Weigh 0.986g of cadmium perchlorate hexahydrate (molecular weight: 419.39, 2.35mmol), dissolve it in 125ml of twice distilled water, add 0.43ml (4.85mmol) of mercaptopropionic acid, and adjust the pH of the solution to 11.2. Put the prepared solution into the three-neck flask I, and blow nitrogen into it for half an hour to drive out the air in the system. The device diagram is as follows image 3 shown. Weigh 0.2g (molecular weight: 436.76, 0.46mol) aluminum telluride powder and place it in the small three-necked bottle II. The weighing process should be rapid to avoid the oxidation and deterioration of aluminum telluride by oxygen in the air, and then continue to pass nitrogen for half an hour Afterwards, quickly inject 15ml of 0.5 mole sulfuric acid that has been passed through nitrogen into the three-necked bottle II with a syringe. Sulfuric acid and aluminum telluride react violently to produce hydrogen tell...

Embodiment 2

[0083] (PAH / CdTe) x (PAH / PSS) y (PAH / GOD) zFluorescence response of glucose sensor with multilayer film structure to glucose, where x, y, z are the repeated coating times of semiconductor quantum dot layer, glucose barrier layer and enzyme layer (PAH / CdTe) 12 (PAH / PSS) 3 (PAH / GOD) 3 The preparation method of the structured multilayer film is as follows:

[0084] a) Prepare a concentrated sulfuric acid / hydrogen peroxide treatment solution, then put the working electrode area of ​​the microelectrode into the above solution, treat it in a constant temperature water bath at 70-80°C for 1.5 hours, take it out of the solution and wash it thoroughly with deionized water. Then put the working electrode area of ​​the microelectrode into the hydrogen peroxide / ammonia water treatment solution, treat it in a constant temperature water bath at 60-70°C for 1.5 hours, and finally rinse it with deionized water; immerse the negatively charged working area in polypropylene amine hydrochlori...

Embodiment 3

[0098] (PAH / CdTe)x(PAH / PSS)y(PAH / GOD)z Multilayer Film Glucose Sensor for Glucose Detection in Calf Serum

[0099] Calf serum was used as the actual sample for (PAH / CdTe) 12 (PAH / PSS) 3 (PAH / GOD) 3 The performance test of the multilayer film structure glucose sensor, according to the fluorescence intensity at 630nm, first get Qm, and then according to Figure 8 The linear relationship graph in the graph gives the corresponding glucose concentration. In the control experiment, a medical biochemical analyzer (Beckman Coulter Unicel DxC600) was used to test the same batch of serum. The test results are listed in Table 1, from which it can be seen that the actual test results are similar to those obtained by the biochemical analyzer.

[0100] Table 1 Test results of glucose concentration in actual serum samples (concentration unit: mM)

[0101]

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Abstract

The invention relates to electrochemical detection and in particular relates to a fluorescent glucose sensor. The fluorescent glucose sensor comprises a base material, wherein a working area is arranged on the base material and is made from indium tin oxide; the surface of the working area is provided with a semiconductor quantum dot modified layer, a glucose hindering layer and an enzyme layer; a micro fiber and a fluorescent signal acquirer are arranged at the bottom of the working area; the micro fiber is used for transmitting light to the surface of the working area; the fluorescent signal acquirer is used for acquiring fluorescent emitted by a semiconductor quantum dot modified by the surface of the working area, and a cadmium telluride quantum dot is taken as the semiconductor quantum dot. The fluorescent glucose sensor is wide in detection range and high in sensitivity and provides stable signals.

Description

technical field [0001] The invention relates to electrochemical detection, in particular to a fluorescent glucose sensor. Background technique [0002] In the 1980s, biosensors that detect indicators with optical signals began to appear, forming optical fiber biosensors. Optical fiber biosensing technology has broad application prospects in medicine, bioengineering, food industry and environmental monitoring. Fiber-optic glucose sensors have extremely broad application prospects in clinical diagnosis, especially in the fields of diabetes diagnosis and basic medical research. At present, various detection methods for glucose have shortcomings such as low sensitivity and slow response, which cannot meet the actual needs. Therefore, it is necessary to study a detection method with high sensitivity and fast response. The traditional optical fiber glucose biosensor based on fluorescence quenching effect uses fluorescent molecules as fluorescent reagents. Fluorescent molecules ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 不公告发明人
Owner MEDTRUM TECH