Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste
A probiotic preparation and fermentation waste liquid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high energy consumption, high cost, complex process, etc., achieve the preservation of nutritional value and shorten the process flow , The effect of saving production costs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0034] The utilization vitamin B of this embodiment 2 The method for preparing probiotic preparations from fermented waste liquid comprises the following steps:
[0035] 1) Pretreatment of fermentation waste liquid: to vitamin B 2 Sodium bicarbonate and calcium hydroxide are added to the fermentation waste liquid, the mass ratio of the sodium bicarbonate and calcium hydroxide is 3:1, and the pH value of the waste liquid is adjusted to 6.5;
[0036] 2) Probiotic inoculation and fermentation: Add probiotic strains to the waste liquid, the probiotic strains are compound probiotic strains of yeast, actinomycetes and filamentous bacteria, and the amount added is 0.5% of the total mass of the waste liquid %, control the temperature of the waste liquid to be 38°C, start fermentation, and stop the fermentation when the pH value of the fermentation liquid reaches 5;
[0037] 3) Collect bacteria or spores: Centrifuge the fermentation broth at a speed of 10000r / min to collect bacteria ...
Embodiment 2
[0041] The utilization vitamin B of this embodiment 2 The method for preparing probiotic preparations from fermented waste liquid comprises the following steps:
[0042] 1) Pretreatment of fermentation waste liquid: to vitamin B 2 Ammonia water is added to the fermentation waste liquid to adjust the pH value of the waste liquid to 5.5;
[0043] 2) Inoculation and fermentation of probiotics: Add probiotic strains to the waste liquid. The probiotic strains are compound probiotic strains of actinomycetes and filamentous bacteria. The amount added is 0.5% of the total mass of the waste liquid. The waste liquid temperature is 41 ℃, start fermentation, when the content of bacteria or spores in the fermentation liquid reaches 1×10 10 When CFU / g, stop the fermentation;
[0044] 3) Collect bacteria or spores: Centrifuge the fermentation broth at a speed of 10,000r / min to collect bacteria or spores; the centrifuged liquid is concentrated and returned to the fermentation waste liquid ...
Embodiment 3
[0047] The utilization vitamin B of this embodiment 2 The method for preparing probiotic preparations from fermented waste liquid comprises the following steps:
[0048] 1) Pretreatment of fermentation waste liquid: to vitamin B 2 Sodium bicarbonate and calcium hydroxide are added to the fermentation waste liquid, the mass ratio of the sodium bicarbonate and calcium hydroxide is 4:1, and the pH value of the waste liquid is adjusted to 7.2;
[0049]2) Inoculation and fermentation of probiotics: Add probiotic strains to the waste liquid, the probiotic strains are Lactobacillus and Bifidobacterium strains, the amount added is 0.5% of the total mass of the waste liquid, and the temperature of the waste liquid is controlled at 39 ℃, start fermentation, when the content of bacteria or spores in the fermentation liquid reaches 1×10 10 When CFU / g, stop the fermentation;
[0050] 3) Collect bacteria or spores: Centrifuge the fermentation broth at a speed of 10000r / min to collect bac...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com