Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste

A probiotic preparation and fermentation waste liquid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high energy consumption, high cost, complex process, etc., achieve the preservation of nutritional value and shorten the process flow , The effect of saving production costs

Inactive Publication Date: 2015-02-25
HENAN UNIV OF SCI & TECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this invention is to provide a 2 Method for preparing probiotic preparation from fermented waste liquid, for vitamin B 2 Secondary utilization of fermentation waste liquid solves the problems of complex process, high energy consumption and high cost in conventional probiotic production methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The utilization vitamin B of this embodiment 2 The method for preparing probiotic preparations from fermented waste liquid comprises the following steps:

[0035] 1) Pretreatment of fermentation waste liquid: to vitamin B 2 Sodium bicarbonate and calcium hydroxide are added to the fermentation waste liquid, the mass ratio of the sodium bicarbonate and calcium hydroxide is 3:1, and the pH value of the waste liquid is adjusted to 6.5;

[0036] 2) Probiotic inoculation and fermentation: Add probiotic strains to the waste liquid, the probiotic strains are compound probiotic strains of yeast, actinomycetes and filamentous bacteria, and the amount added is 0.5% of the total mass of the waste liquid %, control the temperature of the waste liquid to be 38°C, start fermentation, and stop the fermentation when the pH value of the fermentation liquid reaches 5;

[0037] 3) Collect bacteria or spores: Centrifuge the fermentation broth at a speed of 10000r / min to collect bacteria ...

Embodiment 2

[0041] The utilization vitamin B of this embodiment 2 The method for preparing probiotic preparations from fermented waste liquid comprises the following steps:

[0042] 1) Pretreatment of fermentation waste liquid: to vitamin B 2 Ammonia water is added to the fermentation waste liquid to adjust the pH value of the waste liquid to 5.5;

[0043] 2) Inoculation and fermentation of probiotics: Add probiotic strains to the waste liquid. The probiotic strains are compound probiotic strains of actinomycetes and filamentous bacteria. The amount added is 0.5% of the total mass of the waste liquid. The waste liquid temperature is 41 ℃, start fermentation, when the content of bacteria or spores in the fermentation liquid reaches 1×10 10 When CFU / g, stop the fermentation;

[0044] 3) Collect bacteria or spores: Centrifuge the fermentation broth at a speed of 10,000r / min to collect bacteria or spores; the centrifuged liquid is concentrated and returned to the fermentation waste liquid ...

Embodiment 3

[0047] The utilization vitamin B of this embodiment 2 The method for preparing probiotic preparations from fermented waste liquid comprises the following steps:

[0048] 1) Pretreatment of fermentation waste liquid: to vitamin B 2 Sodium bicarbonate and calcium hydroxide are added to the fermentation waste liquid, the mass ratio of the sodium bicarbonate and calcium hydroxide is 4:1, and the pH value of the waste liquid is adjusted to 7.2;

[0049]2) Inoculation and fermentation of probiotics: Add probiotic strains to the waste liquid, the probiotic strains are Lactobacillus and Bifidobacterium strains, the amount added is 0.5% of the total mass of the waste liquid, and the temperature of the waste liquid is controlled at 39 ℃, start fermentation, when the content of bacteria or spores in the fermentation liquid reaches 1×10 10 When CFU / g, stop the fermentation;

[0050] 3) Collect bacteria or spores: Centrifuge the fermentation broth at a speed of 10000r / min to collect bac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste. The method comprises following step: 1) the pH value of the vitamin B2 fermentation liquid waste is adjusted; 2) probiotic strain is added into the vitamin B2 fermentation liquid waste for fermentation, and fermentation is stopped when the pH value of the fermentation broth reaches 5 or the amount of probiotic or spore reaches a certain value; 3)the fermentation broth is subjected to centrifugal separation so as to collect probiotic or spore; and 4) probiotic or spore is subjected to vacuum drying so as to obtain the finished products. According to the method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste, probiotic strain is inoculated directly, processes of preparation and high-temperature disinfection of medium are avoided, technological process is shortened, discharge is not increased, work efficiency is increased, production cost is reduced, labor and raw materials are reduced, and energy saving and emission reduction are realized. The probiotic preparations prepared by using the method can be added into forage so as to realize disease prevention and treatment, and increase utilization rate of forage; and is capable of taking place of antibiotic.

Description

technical field [0001] The invention belongs to the technical field of fermentation waste liquid treatment, in particular to a method of using vitamin B 2 A method for preparing a probiotic preparation from fermentation waste liquid. Background technique [0002] Vitamin B 2 Riboflavin, also known as riboflavin, is one of the indispensable vitamins in biological life activities. It is closely related to the metabolism of carbohydrates, fats and amino acids in the human body, and plays an extremely important role in the growth and development of organisms. Vitamin B 2 Fermentation waste liquid refers to vitamin B 2 During production, vitamin B 2 Residue waste after distillation. The waste liquid has a solid content of about 5%, contains a large amount of nutrients such as sugar, protein, ammonia nitrogen, etc., which are often needed for the growth of microorganisms, and is a sterile liquid. [0003] In the prior art, vitamin B 2 The treatment method of the fermentatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/16C12N1/20C12R1/01C12R1/225
Inventor 张光勤罗来强位兰李道敏李元晓吴庭才左成志吴晓红李旺粟颖华赵芙蓉
Owner HENAN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products