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Rat spinal cord full-transection injury model and method for establishing rat spinal cord full-transection damage model

An injury model and method of establishing a technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problem of uneven size of the injury area, ischemic injury, lateral displacement of the spinal cord, etc., to achieve consistent injury standards, High reliability and good repeatability

Inactive Publication Date: 2014-01-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems in Allen's method, such as the lateral deviation of the spinal cord at the moment of contusion, resulting in asymmetric spinal cord injury, and the size of the injured area is not constant
Animals show wide variation in degree and duration of paralysis, making it difficult to maintain consistent levels of injury
[0006] The model of spinal cord ischemia-reperfusion injury is mostly caused by blocking the abdominal aorta or transperitoneally blocking the left renal artery and the inferior abdominal aorta. The disadvantage of this model is that it causes extensive ischemia in the abdominal cavity and lower limbs at the same time. injury, which undoubtedly affects the assessment of behavior and function after spinal cord injury and treatment
The disadvantage of this model is that it is difficult to keep the clamping position and clamping force consistent, making it difficult to control and evaluate

Method used

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  • Rat spinal cord full-transection injury model and method for establishing rat spinal cord full-transection damage model
  • Rat spinal cord full-transection injury model and method for establishing rat spinal cord full-transection damage model
  • Rat spinal cord full-transection injury model and method for establishing rat spinal cord full-transection damage model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Modeling of Rat Spinal Cord Transection Injury Model

[0051] SD rats (Sprague Dawley Rat), both male and female, weighing 250-300g, were randomly divided into experimental group and control group, and the rats in the experimental group were injected intraperitoneally with a mass percentage of 0.3 ml / 100 g rat body weight with a mass percentage of 10% Anesthetized with chloral hydrate, fixed the limbs of the rat on a square wooden board with 4 nails on both sides, prepared the skin of the corresponding thoracic vertebra segment 7-12, disinfected it with 2.5% povidone-iodine by mass volume percentage, and cut it longitudinally The skin on the back, the incision length is about 4 cm, the subcutaneous tissue of the back is bluntly separated, and the subcutaneous tissue is retracted with a mastoid retractor (14 cm, fixed 3×4 hook, blunt handle), and the subcutaneous tissue is retracted with a mastoid rongeur (9 cm) , spear-shaped, with a head width of 3 mm, and a ...

Embodiment 2

[0053] Example 2 The motor function test of the total transection injury model of the rat spinal cord in Example 1

[0054] 1. Method

[0055] 1.1 Observe the situation of the model animal of embodiment 1

[0056] 1.2 Carry out BBB score to the model animal of embodiment 1

[0057] To evaluate the recovery of the motor function of the hindlimbs of the rats, the animals were placed on the platform, and the walking and limb activities of the hindlimbs were observed and recorded. The score is divided into three parts, the first part is 0-7 points, judging the joint activities of the animal's hind limbs, the second part is 8-13 points, judging the gait and coordination function of the hind limbs, and the third part is 14-21 points, judging the movement For the fine movements of the middle paw, the full score of the three items is 21 points. Specifically:

[0058] 0 points: no visible hindlimb movement;

[0059] 1 point: Minor movement in one or two joints, usually hip and / or ...

Embodiment 3

[0089] Example 3 Histological changes of the spinal cord in the rat spinal cord total transection injury model of Example 1

[0090] 1. Preparation of spinal cord specimens

[0091] In the experimental group and the control group of Example 1, 5 rats were taken from each group. One week after the operation, they were intraperitoneally anesthetized with 10% chloral hydrate by mass percentage, fixed in supine position, and cut open the chest cavity to expose the heart. The ventricle was quickly perfused with normal saline through the venous catheter, and when the outflow from the right atrial appendage was basically colorless, it was perfused and fixed with 4% paraformaldehyde by mass. After 30 min, the spinal cord tissue was isolated, and the injury and adjacent segments of about 1 cm were removed. Fix with 4% paraformaldehyde at 4°C for 24 hours, transfer to 30% sucrose solution by mass until it sinks to the bottom, and then perform frozen sections for HE staining (hematoxyli...

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Abstract

The invention discloses a rat spinal cord full-transection injury model and a method for establishing the rat spinal cord full-transection injury model. The method comprises the steps of injecting anesthetic, fixing four limbs of a rat, (2) preserving skin, longitudinally cutting the skin of the back open, bluntly dissecting the subcutaneous tissues of the back, exposing corresponding sections of the spine, (3) conducting the laminectomy to fully expose the spine, pricking up the spine, completing transverse cutting, (4) suturing the wound in a layer-by-layer mode, disinfecting the surface of the wound, binding up the surface of the wound, immediately injecting penicillin to the muscle of the rear leg, and (5) conducting postoperative nursing. Compared with an existing rat spinal cord modeling method, the method for establishing the rat spinal cord full-transection injury model has breakthroughs and improvement on selection of the injury sections, the surgical operation method, selection of adopted instruments and the postoperative nursing, the model obtained through the method has the advantages of being high in reliability, good in repeatability and consistent in injury standard, and the method is of great importance in research on the pathomechanism of spinal cord injuries and discussion on new therapeutic schedules.

Description

technical field [0001] The invention belongs to the technical field of medical animal models, in particular to a rat spinal cord total transection injury model and a method for establishing the same. Background technique [0002] Spinal cord injury refers to the corresponding changes of various motor, sensory and sphincter dysfunction, abnormal muscle tone and pathological reflexes in the corresponding segment of the spinal cord injury caused by external direct or indirect factors. One of the world's difficult diseases. Spinal cord injury has become an urgent problem to be solved, showing the characteristics of high incidence, high disability rate, high cost and low mortality, seriously affecting people's quality of life. So far, the methods to treat and promote the rehabilitation of spinal cord injury mainly rely on drug therapy and surgical treatment. Due to the complex pathology of spinal cord injury, these methods have alleviated the pathological changes of spinal cord...

Claims

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Application Information

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IPC IPC(8): A61D1/00
Inventor 田卫东郭维华杨超李星翰孙亮
Owner SICHUAN UNIV
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