Extraction process of phosphatidylcholine with antarctic krill as source and preparation method of phosphatidylserine

A technology of phosphatidylserine and phosphatidylcholine, which is applied in the extraction process of phosphatidylcholine from Antarctic krill and the preparation of phosphatidylserine, can solve the problems of high cost, improve the operating environment and save production costs , easy maintenance effect

Inactive Publication Date: 2014-01-15
ACERCHEM INT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Carbon dioxide supercritical is a relatively feasible means for the experimental and theoretical stages, but it needs an investment of up to hundreds of millions of RMB to truly form a set of equipment for industrialization
According to the inventor's research, th...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The preparation of embodiment 1 phosphatidylserine 1

[0061] (1) Put 2000kg of Antarctic krill heads into a 5-ton alcohol extraction frying pan, pour 2000L of water into it, and heat it to 50°C. For the convenience of subsequent cold pressing, spray 5% edible ethanol while stirring. The weight percentage of edible ethanol can be 3-6%, generally 5% is the best, which can increase the lubrication of the press. Stir for 10 minutes, then transfer the krill head decoction to a cold press (model 6YL-165, Henan Zhongkuang) for solid-liquid separation. The processing capacity of the cold press is 1000kg / h, the speed is 28-35r / min, and the power is 30kw , to obtain oily liquid rich in phospholipids and solids such as krill heads;

[0062] (2) Put the solid obtained in (1) into the reactor, add an anhydrous ethanol solvent whose mass is 4 times that of the obtained solid, and stir at high shear for 20 minutes (15~30 minutes are acceptable), so that the phospholipid component is...

Embodiment 2

[0073] The preparation of embodiment 2 phosphatidylserine 2

[0074] (1) Put 2050kg of Antarctic krill heads into a 5-ton alcohol extraction frying pan, pour into 2000L of water, and heat to 55°C. For the convenience of subsequent cold pressing, spray a small amount of 6% ethanol while stirring, stir for 10 minutes, and then put the phosphorus The shrimp head decoction liquid is sent to the cold press (model 6YL-165, Henan Zhongkuang) for solid-liquid separation. The processing capacity of the cold press is 1000kg / h, the speed is 28-35r / min, and the power is 30kw to obtain phospholipid-rich Oily liquids and solids such as krill heads;

[0075] (2) Put the solid obtained in (1) into a reactor, add an anhydrous ethanol solvent whose mass is 3 times that of the obtained solid, and stir at high shear for 15 minutes, so that the phospholipid component is fully dissolved in ethanol and centrifuged; Put it into the reactor and add an anhydrous ethanol solvent whose mass is 3 times t...

Embodiment 3

[0086] The preparation of embodiment 3 phosphatidylserine 3

[0087] (1) Put 2010kg of Antarctic krill heads in a 5-ton alcohol extraction frying pan, pour into 2000L of water, and heat to 45°C. For the convenience of subsequent cold pressing, spray a small amount of 3% ethanol while stirring, stir for 10 minutes, and then put the phosphorus The shrimp head decoction liquid is sent to the cold press (model 6YL-165, Henan Zhongkuang) for solid-liquid separation. The processing capacity of the cold press is 1000kg / h, the speed is 28-35r / min, and the power is 30kw to obtain phospholipid-rich Oily liquids and solids such as krill heads;

[0088] (2) Put the solid obtained in (1) into a reactor, add an anhydrous ethanol solvent whose mass is 3 times that of the obtained solid, and stir at high shear for 15 minutes, so that the phospholipid component is fully dissolved in ethanol and centrifuged; Put it into the reactor and add an anhydrous ethanol solvent whose mass is 3 times tha...

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Abstract

The invention provides an extraction process of phosphatidylcholine with antarctic krill as a source. The extraction process comprises the following steps: collecting heads of the antarctic krill, adding water for decoction, stirring, sending the krill head decoction liquid to a cold pressing machine for solid-liquid separation, to obtain an oily liquid rich in phospholipid and krill heads and other solids; adding absolute ethyl alcohol into the krill heads and other solids, carrying out high shearing extraction, carrying out centrifugal separation, and concentrating to obtain an oily liquid; merging the oily liquids, drying under a reduced pressure, then filtering through a plate frame filter to remove insoluble substances, followed by carrying out membrane separation and concentration, carrying out column chromatography separation and purification, carrying out freeze drying, and thus obtaining the phosphatidylcholine product. The invention also provides a preparation method for phosphatidylserine with the antarctic krill as the source. The preparation method comprises the steps of hydrolyzing the extracted phosphatidylcholine by phospholipase D, and extracting to obtain phosphatidylserine rich in DHA and EPA. The extraction process and the preparation method are environmentally friendly, and are low in cost; and the obtained products have high content and good activity, and can be industrially produced.

Description

technical field [0001] The invention relates to a deep processing method from Antarctic krill, in particular to a method for extracting phosphatidylcholine and preparing phosphatidylserine from Antarctic krill. Background technique [0002] Phosphatidylserine (PS) rich in unsaturated fatty acid docosahexaenoic acid (DHA) has a broad market prospect as an emerging health care and pharmaceutical raw material for preventing senile dementia, preventing ADHD and other brain health and preventing cardiovascular disease in the future. [0003] Antarctic krill is a type of krill that lives in the waters of Antarctica and is one of the largest and most successful single species of biological resources on Earth. Krill oil extracted from Antarctic krill is rich in functional components such as phospholipids (mainly lecithin), and the fatty acid composition of phospholipids is eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). ) mainly, EPA and DHA content accounted for more t...

Claims

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Application Information

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IPC IPC(8): C07F9/10C12P13/04
Inventor 蒋锋锋包琦
Owner ACERCHEM INT
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