Silkworm chrysalis active polypeptide after-sun repairing gel and preparation method thereof
A silkworm chrysalis active polypeptide, after-sun repair technology, applied in the field of skin care products, can solve the problems of not easy skin absorption, poor solubility of globulin, allergies in the user group, etc., to reduce the possibility of UV damage and cancer, eliminate allergic factors, reduce The effect of melanin deposition
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Embodiment 1
[0084] (1) Preparation of silkworm chrysalis active polypeptide:
[0085] (1) Prepare a silkworm chrysalis protein solution with a concentration of 12% with deionized water, add papain, the enzyme amount is 20000IU / g silkworm chrysalis protein, enzymolysis temperature is 55°C, pH value is 7.0, enzymolysis time is 3h, and 92 ℃ inactivate the enzyme for 13 minutes;
[0086] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.15KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;
[0087] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed through an ultra-microfiltration membra...
Embodiment 2
[0117] (1) Preparation of silkworm chrysalis active polypeptide:
[0118](1) Prepare a silkworm chrysalis protein solution with a concentration of 10% in deionized water, add papain, the enzyme amount is 8000IU / g silkworm chrysalis protein, the enzymolysis temperature is 55°C, the pH value is 7.0, the enzymolysis time is 3h, and the enzymolysis time is 92 ℃ inactivate the enzyme for 13 minutes;
[0119] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.075KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;
[0120] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed throug...
Embodiment 3
[0128] (1) Preparation of silkworm chrysalis active polypeptide:
[0129] (1) Prepare a silkworm chrysalis protein solution with a concentration of 15% in deionized water, add papain, the enzyme amount is 60000IU / g silkworm chrysalis protein, enzymolysis temperature is 55°C, pH value is 7.0, enzymolysis time is 3h, and the enzymolysis time is 92 ℃ inactivate the enzyme for 13 minutes;
[0130] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.225KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;
[0131] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed through an ultra...
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