Anti-liver cell aging co-fixation electric biomaterial and application thereof

A technology of biomaterials and hepatocytes, applied in the field of co-immobilization of electrical biomaterials, can solve problems such as no related reports.

Active Publication Date: 2015-07-08
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no relevant report on the research on biomaterials for delaying the aging of liver cells

Method used

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  • Anti-liver cell aging co-fixation electric biomaterial and application thereof
  • Anti-liver cell aging co-fixation electric biomaterial and application thereof
  • Anti-liver cell aging co-fixation electric biomaterial and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Preparation of photofixed electrical material AzPhPAAc-Pst

[0066] Take 6.0mmol 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (WSC), 1.0mmol PAAc and 0.1mmol 4-azidoaniline hydrochloride dissolved in 110ml deionized water ; The pH of the solution was adjusted to 7.0 with NAOH or HCL, and after stirring at 4°C for 48 h, the reaction solution was dialyzed with ultrapure water through a seamless cellulose tube (cutoff MW, 12000) until the azidophenyl group in the washing liquid The content cannot be detected by ultraviolet spectrophotometer, and the derivative AzPhPAAc modified by azidophenyl group is obtained, and the monolayer polymer is freeze-dried. Under a dark environment, the obtained derivatives were formulated into a 1 mg / ml aqueous solution, and the resulting solution was placed in a 24-well polystyrene (PSt) culture plate (10 μl per well), and air-dried at room temperature in the dark. 5 ΜJ / cm 2 The intensity of ultraviolet light w...

Embodiment 2

[0067] Example 2 Preparation of photofixed electrical material AzPhPEG-Pst

[0068] 100 mg of diaminoPEG and 81 mg of N-(4-azido)succinimide were stirred overnight in chloroform to obtain a product which was purified three times by chloroform / dehydrated ether until 4-azidosuccinimide in the eluent It cannot be detected by ultraviolet spectrophotometer, and the derivative AzPhPEG modified by azidophenyl group is obtained. In a dark environment, the obtained derivatives were prepared into 1 mg / ml aqueous solution, and the obtained solution was placed in a 24-well polystyrene culture plate (PSt plate) (10 μl per well), and air-dried at room temperature in the dark. 5 ΜJ / cm 2 The intensity of ultraviolet light was irradiated from a distance of 15cm for 60 seconds, and the culture plate was placed in the solution of PH=4, the solution of PH=10 and ultrapure water, and the unreacted polymer was removed by ultrasonic wave. After washing, the plate was washed with Sterilized with...

Embodiment 3

[0069] Example 3 Preparation of photofixed electrical material AzPhPAAm-Pst

[0070] In 50ml of THF solution with 13.3g of DCCI dissolved, 150ml of THF solution with 13.3g of hydroxysuccinimide dissolved and 9.57g of 4-azidobenzoic acid were added dropwise, and the process was stirred in an ice bath; after 3 hours, the reaction solution slowly warmed up Keep stirring overnight at room temperature; the formed white liquid is filtered, and the remaining yellow residue is crystallized in isopropyl alcohol / isopropyl ether; then 10ml of an aqueous solution with 30mg of PAAm dissolved, pH 7.0, in an ice bath Add 20ml of DMF solution dissolved with 8.4mg of 4-azidosuccinimide while stirring, and after stirring at 4° for 24h, the solution is ultrafiltered and washed with 5ml of DMF / H 2 O solution was washed twice, and then 5 ml of ultrapure water was used until 4-azidosuccinimide in the eluate could not be detected by ultraviolet spectrophotometer, and the derivative AzPhPAAm modifi...

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Abstract

The invention discloses a co-immobilized electrical biomaterial for anti-aging of liver cells and its application. It is prepared by the following steps: using a photochemical immobilization method to graft a charged electrical material on a polymer culture plate to obtain a photoimmobilized electrical material ; Then, by co-grafting TNF-a and IGF on the photofixed electrical material, the co-immobilized electrical biomaterial is obtained by ultraviolet irradiation; the charged electrical material is negatively charged polyacrylic acid (PAAc) , electrically neutral polyethylene glycol (PEG), and positively charged polyacrylamine (PAAm). The co-immobilized electrical biomaterial prepared by the invention can not only improve the survival rate of human hepatocyte HSC, but also effectively inhibit the aging of HSC cells.

Description

technical field [0001] The invention relates to the technical field of biomaterials, more specifically, to co-immobilized electrical biomaterials for anti-aging of liver cells and applications thereof. Background technique [0002] The liver is an important organ of the human body and the material metabolism center of the human body. It has functions such as metabolism, detoxification, and circulation regulation. However, liver cancer, acute liver failure (such as fatty liver, cirrhosis, hepatitis) and other diseases cause a large number of deaths every year, posing a serious threat to human health. Since the liver is a unique organ in the human body, autologous transplantation cannot be performed; and allogeneic transplantation will cause tissue immune rejection, and the failure of anti-rejection treatment will mean that all previous efforts have been wasted; and limited by the source of organ supply, liver donors are in short supply, making it difficult to meet clinical ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/12A61L27/54A61L27/50A61L27/16
Inventor 关燕清刘俊明张琳吴丽芳
Owner SOUTH CHINA NORMAL UNIVERSITY
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