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Rapid preparation method of rod-like nano-hydroxyapatite

A nano-hydroxyapatite, rod-shaped technology, applied in the direction of nanotechnology, nanotechnology, chemical instruments and methods, etc., can solve the problems of increasing the toxicity of hydroxyapatite, cumbersome methods, etc., achieve uniform and controllable morphology, and the preparation method Simple, mild and controllable results

Inactive Publication Date: 2014-01-29
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Chinese invention patent application with publication number CN102515128A discloses a preparation method of rod-shaped hydroxyapatite, but the method is relatively cumbersome, and the N,N-dimethylformamide used can be absorbed through the respiratory tract or skin after volatilization , cause human poisoning after entering the digestive tract, increase the toxicity of hydroxyapatite, and limit the application of nano-hydroxyapatite prepared by its method to gene or drug carriers

Method used

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  • Rapid preparation method of rod-like nano-hydroxyapatite
  • Rapid preparation method of rod-like nano-hydroxyapatite
  • Rapid preparation method of rod-like nano-hydroxyapatite

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Experimental program
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Embodiment 1

[0025] 1) Take 3 ml of 0.09 M DTAB solution, add DTAB solution into 177ml of deionized water, stir at 300 rpm for 30 minutes until DTAB is evenly dispersed in the aqueous solution, and the final concentration of DTAB is 1.5×10 -3 mol / L.

[0026] 2) At a temperature of 30 or 40°C, add 60ml of 0.03M Na 2 HPO 4 The aqueous solution was added to the above solution, stirred at a stirring rate of 200 rpm for 30 minutes, and the pH value of the mixed solution was adjusted to 11 with 1M NaOH.

[0027] 3) Add 60ml, 0.05M CaCl 2 The aqueous solution is added dropwise to the above solution, so that the Ca / P molar ratio of the final reaction system is 1.67, and the dropping rate is 20 or 50 drops / min. During the dropping process, NaOH solution is added dropwise to maintain the pH value of the reaction system at 10 , maintain the temperature at 60° C., and maintain the stirring rate at 300 rpm.

[0028] 4) After the dropwise addition, continue to stir for 12 hours, and then react for ...

Embodiment 2

[0031] 1) Take 5ml of 0.09M DTAB solution, add it to 175ml of deionized water, stir at a stirring rate of 500 rpm for 30 minutes until DTAB is evenly dispersed in the aqueous solution, and the final concentration of DTAB is 2.5×10 -3 mol / L.

[0032] 2) At a temperature of 50 or 70°C, add 100ml of 0.03M Na 2 HPO 4 Aqueous solution was added to the above solution, stirred at a stirring rate of 400 rpm for 30 minutes, and the pH value of the mixed solution was adjusted to 11 with 1M NaOH.

[0033] 3) Add 100ml, 0.05M calcium nitrate aqueous solution dropwise to the above solution, so that the Ca / P molar ratio of the final reaction system is 1.67, and the dropping rate is 30 drops / min. During the dropping process, add NaOH solution dropwise The pH of the reaction system was maintained at 12, the temperature was maintained at 70° C., and the stirring rate was maintained at 400 rpm.

[0034] 4) After the dropwise addition, continue to stir for 8 hours, and then react for 2 hours ...

Embodiment 3

[0037] 1) Take the prepared rod-shaped nano-hydroxyapatite powder, sterilize it under ultraviolet light for 2 hours, and add an appropriate amount of cell culture medium under sterile conditions to ultrasonically resuspend to make the concentration 1mg / mL;

[0038] 2) Spread QSG-7701 cells (normal human liver cells) in a 96-well plate at a concentration of 10,000 cells per well. When the cells enter the exponential growth phase, add the above-mentioned HAp particles, and add them to the HAp wells The final concentrations are 0.005, 0.01, 0.025, 0.05, 0.1 (mg / mL).

[0039] 3) After 48 hours, add 20 microliters of MTT (5 mg / mL) to each well, and after adding MTT for 4 hours, remove the supernatant completely, add 150 microliters of DMSO to each well, shake gently for 12 minutes, and test its presence in the microplate reader. The absorbance value at 490nm was determined by origin8.0 Figure 5 .

[0040] 4) Select the HAp concentration under the optimal cell survival rate, and ...

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Abstract

The invention discloses a rapid preparation method of rod-like nano-hydroxyapatite. The method comprises the following steps: (1) reacting dodecyl trimethyl ammonium bromide with disodium hydrogen phosphate; (2) dropwise adding a calcium salt solution into the mixed solution obtained in the step (1) to react; (3) continuing the reaction at the temperature of 120 DEG C; (4) carrying out postprocessing. According to the preparation method of the rod-like nano-hydroxyapatite, disclosed by the invention, the dodecyl trimethyl ammonium bromide serves as a regulating agent; the preparation method is simple, mild and controllable in condition; the rod-like nano-hydroxyapatite with a uniform and controllable size and shape can be obtained. In addition, the dodecyl trimethyl ammonium bromide has good biocompatibility; proven by cell experiments, the rod-like nano-hydroxyapatite provided by the invention can be used for the application research of gene and drug carriers.

Description

technical field [0001] The invention belongs to the technical field of nanometer materials, and in particular relates to a rapid preparation method of rod-shaped nanometer hydroxyapatite. Background technique [0002] Gene therapy is an effective treatment for cancer and genetic diseases. The key to current research is to find new gene therapy carriers to transport the target gene to the target site to achieve the purpose of treating the disease. There are two types of existing gene therapy vectors: viral vector systems and non-viral vector systems. The virus vector system has a high transfection efficiency, but it is also potentially immunogenic, has potential safety hazards and high costs, and has caused the death of patients many times in clinical applications. Therefore, the safety of viruses as gene therapy vectors been disputed many times. [0003] In order to avoid the above disadvantages, researchers began to turn to non-viral vectors, among which nano-hydroxyapati...

Claims

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Application Information

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IPC IPC(8): C01B25/32B82Y30/00B82Y40/00
Inventor 孔祥东赵瑞波杨新燕张祺任小元谢纯刚
Owner ZHEJIANG SCI-TECH UNIV
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