Method for producing silkworm chrysalis peptide powder and silkworm chrysalis meal by aqueous biological enzyme method

Abstract

The invention relates to a method for producing silkworm chrysalis peptide powder and silkworm chrysalis meal by an aqueous biological enzymatic method. In the method, silkworm chrysalis is used as a raw material, and an enzymatic hydrolysis liquid is obtained through drying and pulverization and aqueous phase enzymatic hydrolysis, and the enzymatic hydrolysis liquid is obtained by centrifugal separation. The supernatant and residue, the residue is dried and crushed to obtain silkworm chrysalis meal, the supernatant is subjected to oil-water separation, ultrafiltration membrane ultrafiltration, desalination treatment, drying and crushing to obtain silkworm chrysalis peptide powder, the specific method includes the following steps: (1) Drying and crushing; (2) Water-phase enzymatic hydrolysis; (3) Centrifugation, oil-water separation; (4) Ultrafiltration membrane filtration; (5) Desalination treatment; (6) Drying and crushing to obtain silkworm chrysalis peptide powder. The present invention is the first to apply the water-phase biological enzyme method to insect cells—silkworm chrysalis cells, which has the advantages of simple process, safety and environmental protection, low cost, and high quality silkworm chrysalis peptide powder and silkworm chrysalis meal. The method of the present invention has high oil removal efficiency, The obtained silkworm chrysalis peptide powder and silkworm chrysalis meal have the characteristics of low residual fat content, high oligopeptide content and the like.

Description

Technical field [0001] The invention involves a method of producing silkworm pupa peptide and silkworm pupae meal in a water phase biological enzyme method, which belongs to the field of biological engineering technology. Background technique [0002] my country is a long history of raising silkworms, silk silk, and silk -making countries. In industrial production, the amount of dry silkworm pupa produces about 200,000 tons each year. Such a huge amount of silkworm pupae resource. Except for a small part of processing as food, most of them are directly baked. After drying, it is used for feed.The content of dry silkworms is about 28.9 ~ 33.1%, and the saving period of silkworm pupae is very short, and long -term preservation can easily produce acid defeat or even mold.At present, the silkworm pupa resources behind the silk are directly used as feed. On the one hand, a large amount of oil resources are wasted, and it is also easy to cause adverse consequences such as yellow piglet...

AI Technical Summary

Problems solved by technology

However, there are mainly two problems as follows: on the one hand, the price of silkworm chrysalis defatted powder is very high, with an average of 18,000 yuan / ton, and the production cost is high; There will be residues of organic solvents involved, which will also bring huge pressure on production, environmental protection and safety.

[0004] Aqueous bio-enzyme method is an emerging ideal method for hydrolyzing oleaginous protein. It breaks the plant cell wall through enzymatic hydrolysis, releases the oil, retains the biological activity of the oil, and has mild action conditions, simple process route, and energy consumption in the production process. Low, but there are difficulties in research and development, low oil yield, and difficulty in demulsification of the emulsion formed by protein and oil after enzymatic hydrolysis. The research on water-phase biological enzyme method is currently a hot spot in the food industry at home and abroad. So far A variety of oils have been tested, but most of them are limited to plant cells, and no relevant research reports have been found on insect cells with high fat content

Images