Method for producing seaweed protein feed through synergistic effect of enzymolysis and fermentation

A seaweed protein, synergistic technology, applied in animal feed, animal feed, application and other directions, can solve the problems of high crude fiber content and low degradation rate of seaweed slag, and achieve the improvement of transformation utilization rate, protein content, and simple process method. Effect

Inactive Publication Date: 2015-04-29
INNER MONGOLIA UNIV FOR THE NATITIES +1
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current high crude fiber content and low degradation rate of seaweed residue, the present invention provides a method for producing biological seaweed protein feed through the synergistic effect of enzymolysis and fermentation, so as to solve the problem of reusing seaweed residue for feed production, especially the nutrition of aquatic feed production Defects, improve the nutritional value of seaweed residue resources reuse

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of bacterial solution: Take 18g of glucose, 12g of peptone, 0.5g of beef extract, 16g of agar, 5.0g of magnesium sulfate, 3.0g of potassium dihydrogen phosphate, 1.0g of sodium chloride and 1000ml of distilled water, mix well, the pH of the medium is 7.0, 120 Sterilize at ℃ for 25 minutes to prepare the strain culture medium; insert 30 g of Candida tropicalis and Bacillus subtilis 40 into the above strain culture medium respectively, and culture at 36°C and 150 rpm on a shaker for 24 hours to prepare the strain culture and propagate Up to 2.5L spare.

[0020] Preparation of exogenous enzyme-fermentation substrate: seaweed slag (water content 38%) and feed enzyme preparation (cellulase: pectinase) are fully mixed according to the ratio of 2g: 7IU: 5IU, the pH value is 6.2, the temperature is 35 Enzymolysis under the condition of ℃ for 7 hours to obtain the seaweed residue-feed enzyme preparation mixture; then take 95% of the seaweed residue-feed enzyme prepar...

Embodiment 2

[0023] Preparation of bacterial solution: Take 20g of glucose, 15g of peptone, 1g of beef extract, 18g of agar, 6.0g of magnesium sulfate, 4.0g of potassium dihydrogen phosphate, 2.0g of sodium chloride and 1000ml of distilled water, mix well, medium pH7.0, 115 Bacteria for 30 minutes, the prepared bacterial liquid, multiplied to 2.5 L for later use, respectively, 50 g of Candida tropicalis and 60 g of Bacillus subtilis were inserted into the above-mentioned strain culture medium, and cultured on a shaking table at 36 °C and 150 rpm for 36 hours to obtain the bacterial liquid , to obtain a bacterial solution, and multiply to 2.5L for later use.

[0024] Preparation of exogenous enzyme-fermentation substrate: seaweed slag (water content 38%) and feed enzyme preparation (cellulase: pectinase) are fully mixed according to the ratio of 2g: 7IU: 5IU, the pH value is 7.0, the temperature is 45 Enzymolysis under the condition of ℃ for 4 hours to obtain the seaweed residue-feed enzyme...

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Abstract

The invention discloses a method for producing a seaweed protein feed through the synergistic effect of enzymolysis and fermentation. The method comprises the steps of fully mixing seaweed residue (the water content is 38%) and a feed enzyme preparation according to a certain proportion and performing the enzymolysis for 4-7h with the pH of 6.2-7.0 at the temperature being 35-45 DEG C to obtain a seaweed residue-feed enzyme preparation mixture; inoculating compound microorganisms and performing solid fermentation. The method has a simple process and is easy to operate; an external source is added to degrade a cellulose preparation to produce nutrition beneficial to microbial growth through the enzymolysis and make up the deficiency of enzyme produced by using the compound microorganisms, so as to stabilize the solid fermentation process and the product quality, greatly reduce the content of cellulose and increase the protein content; the seaweed protein feed is rich in multiple minerals, bioactive constituents and unknown somatomedin, and can be used as a protein feed for livestock and poultry and an aquatic feed; the method provides a technical support for growing tensions on developing novel protein resources and relieving a feed protein source.

Description

technical field [0001] The invention belongs to the field of feed protein source production, and in particular relates to a method for synergistically producing biological seaweed protein feed through enzymolysis and fermentation. Background technique [0002] The current shortage of high-quality feed is a well-known objective reality. The demand for protein feed resources in my country is increasing and the dependence on foreign countries is increasing year by year. The shortage of feed protein raw materials has made the problems of my country's feed industry and aquaculture industry, especially aquaculture industry, increasingly prominent. The development and utilization of new feed protein sources and unconventional animal and plant protein feeds is undoubtedly a good strategy to alleviate the shortage of protein feed resources and reduce costs. Therefore, the high-efficiency conversion and utilization technology of protein feed resources based on microbial fermentation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/14A23K1/165A23K1/00A23K1/16
Inventor 牛化欣常杰于建华
Owner INNER MONGOLIA UNIV FOR THE NATITIES
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