Production method and application of compound enzyme liquid for bagasse papermaking pulping
A compound enzyme liquid and production method technology, applied in the fields of paper pulping and bagasse paper pulping, can solve the problems of unfavorable industrial production, difficult control of component damage, and single enzyme species, so as to improve accessibility and utilization rate, The effect of reducing COD content and improving product quality
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Embodiment 1
[0017] A production method of a compound enzyme solution for bagasse papermaking and pulping. The enzymes of each component are weighed, mixed and prepared in a compound tank, preserved, inspected, and packaged, and then stored in a warehouse at 5°C. The enzymes of each component are alkali The alkaline pectinase, xylanase and mannanase; the alkaline pectinase is CCTCC NO: M 2010004 strain culture, shake flask fermentation, seed tank to fermenter, filtration, sterilization, Prepared after antiseptic treatment; the xylanase is prepared after CGMCC NO: 5466 bacterial strain through strain culture, shake flask fermentation, seed tank to fermenter, filtration, sterilization, and antiseptic treatment; the mannanase is CGMCC NO: 6226 strains are prepared by strain culture, shake flask fermentation, seed tank to fermenter tank, filtration, sterilization and antiseptic treatment; the activity of alkaline pectinase in the compound enzyme solution is 800u / ml~1200u / ml, the activity of x...
Embodiment 2
[0053] The various component enzymes described in the present invention are alkaline pectinase, xylanase, mannanase and ligninase; the ligninase is CGMCC NO: 6225 bacterial strain through bacterial strain cultivation and shake flask fermentation, Preparation from seed tank to fermentation tank, filtration, sterilization and antiseptic treatment; the activity of alkaline pectinase in the compound enzyme liquid is 900u / ml-1200u / ml, and the activity of xylanase is 1000u / ml-2500u / ml, the activity of mannanase is 2000u / ml~3000u / ml, and the activity of ligninase is 150u / ml~300u / ml.
[0054] The CGMCC NO: 6225 bacterial strain described in the present invention is collected in Ningxia, China. During the strain selection and screening process, a high-yielding bacterial strain with a laboratory number of KERR7989 was screened out. According to the main physiological and biochemical characteristics and 16S rRNA gene sequence, The gyrB gene sequence and other experimental data were comp...
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