Method for extracting urease inhibiting ingredient from glossy privet leaf and application of urease inhibiting ingredient

An extraction method, chastity leaf urease technology, applied in the agricultural field, can solve the problems of reduced fertilizer efficiency and only 30-50% fertilizer efficiency, achieve good effect, reduce production cost, and improve extraction efficiency

Inactive Publication Date: 2014-02-26
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when urea is applied to the soil, it is decomposed into ammonium carbonate by urease, and the crops have no time to absorb it, and the ammonium carbonate volatilizes in the form of ammonia, resulting in a decrease in fertilizer efficiency. Generally, the fertilizer efficiency of urea is only 30-50%.

Method used

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  • Method for extracting urease inhibiting ingredient from glossy privet leaf and application of urease inhibiting ingredient
  • Method for extracting urease inhibiting ingredient from glossy privet leaf and application of urease inhibiting ingredient
  • Method for extracting urease inhibiting ingredient from glossy privet leaf and application of urease inhibiting ingredient

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, Ligustrum leaf inhibits urease active ingredient extraction process condition test

[0034] 1. The influence of different microwave power on the extraction of urease inhibitors

[0035]Accurately weigh 10 parts of privet leaf powder, each 1.5g, divide into two groups, treat 2 bottles for each solid-liquid ratio, extract with 60% ethanol, feed with a solid-liquid ratio of 1:20, and use different microwave power (119W , 231W, 385W, 539W, 700W) for 2 minutes, then water extraction, water extraction temperature 60 ℃, time 30 minutes, shake gently every 10 minutes. The crude liquid obtained from extraction was centrifuged (5000r / min, 15min) to obtain the extraction supernatant, which was bathed in water at 70°C for 5min. Pipette 2 mL of supernatant into a test tube, add 1.0 mL of urease extract, shake well, leave at room temperature for 1 hour, add 4 mL of citrate buffer (pH 6.7) and 1 mL of 0.025 g / mL urea solution, At the same time, take the control group w...

Embodiment 2

[0046] Example 2. Optimization of Extraction Process Conditions of Ligustrum Leaf Inhibiting Urease Active Components (Orthogonal Test)

[0047] 1. Experimental principle

[0048] According to the analysis of the results of single factor experiment, the influence degree of each factor is very different. In order to explore the interaction between each factor, an orthogonal experiment is designed for research. According to the results of the single factor experiment, the top three of each factor with different levels are obtained. If a comprehensive experiment is performed, it will take 3 4 = 81 trials, while L9 (3 4 ) to do it only 9 times.

[0049] 2. Experimental operation:

[0050] Table 1 Orthogonal experimental design

[0051]

[0052] Accurately weighed 18 parts of privet leaf powder, each 1.5g, divided into 2 groups, each group processed two bottles, processed according to the order of 4 factors 3 levels L9(34) orthogonal experiment table, extracted under microwa...

Embodiment 3

[0084] Embodiment 3, privet leaves inhibit urease active ingredient identification

[0085] Analysis of preliminary detection results of active ingredients inhibiting urease

[0086] After nine color reactions, the results are shown in Table 3

[0087] Table 3 The identification results of the substance components in the ethanol eluent

[0088]

[0089] "+" indicates that it contains relevant chemical substances, and "—" indicates that it does not contain relevant chemical substances.

[0090] It can be seen from the above table 3 that the components in the ethanol eluate do not contain proteins, amino acids, saponins, anthraquinones, cardiac glycosides, alkaloids and other substances, but there are sugars, phenols and some flavonoids. In 2003, Qiu Yexian, Wang Jinlian, Chen Shang and others studied the inhibition of tea polyphenols on urease in red soil paddy fields, and in 2009 Wang Jinlian, Qiu Yexian, Chen Hongwei and others studied the inhibitory effect of tea polyph...

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Abstract

The invention discloses a method for extracting a urease inhibiting ingredient from glossy privet leaf and an application of the urease inhibiting ingredient. The invention provides the method for extracting the urease inhibiting ingredient from the glossy privet leaf and the application thereof. The method comprises the following steps: carrying out crude extraction by taking the glossy privet leaf as a raw material and taking an organic solvent as a solvent combined with microwave, purifying and extracting the urease inhibiting ingredient namely a urea synergist by acrylamide chromatography, wherein the urea synergist is used for an experiment that urease is inhibited to improve utilization of urea nitrogen. Results show that the enzyme inhibition rate achieves 71.2%, and the utilization of the urea nitrogen is improved.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and relates to an extraction method and application of urease-inhibiting components in privet leaves. Background technique [0002] As early as the 1940s, it was discovered that adding certain compounds that can inhibit urease activity to the soil can delay the hydrolysis of urea. In the 1960s, people began a large number of urease inhibitor screening work, and found that aminocarbamate, boron-containing compounds, urea derivatives, heavy metals with an atomic weight greater than 50, fluorine-containing compounds, polyphenols, polyquinones, and anti-metabolites have negative effects on urease activity. It has inhibitory effect; Burn, R.G (1978) believed that phenols and quinones are relatively promising urease inhibitors, and the effect of polyphenolic quinones is better than that of single phenolic quinones; Bremner et al. (1971) confirmed that phenolic quinones inhibited urease The effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/08A01C21/00C05G3/90
Inventor 邱业先汪金莲黄凯玲
Owner SUZHOU UNIV OF SCI & TECH
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