L-carnitine molecularly imprinted microspheres with core-shell structure and preparation method thereof

A technology of molecularly imprinted microspheres and molecularly imprinted membranes, which is applied in the field of core-shell structure molecularly imprinted nano-microspheres and their preparation, can solve problems such as muscle atrophy and muscle soreness, and achieve the effects of low production cost and simple preparation process.

Inactive Publication Date: 2014-03-05
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have shown that only L-carnitine has physiological activity, while D-carnitine has a competitive inhibitory effect on carnitine acetyltransferase (CAT) and carnitine fatty acyltransferase (PTC), inhibiting mitochondrial fatty acid oxidation and energy production. Formation, overdose may also cause muscle soreness, which may seriously cause muscle atrophy. Therefore, with the increase of consumers' safety awareness, DL-carnitine has been eliminated, and the production of optically pure L-carnitine has been paid more and more attention.

Method used

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  • L-carnitine molecularly imprinted microspheres with core-shell structure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1.SiO 2 Preparation of nanospheres: 60ml of absolute ethanol, 9ml of ammonia, 9ml of deionized water were added to a 250ml three-necked flask, and mechanically stirred for 10 minutes to make the solution evenly mixed. Use a syringe to add 4.5ml of ethyl orthosilicate ( TEOS). With the addition of TEOS, the solution gradually turned into a white turbid liquid, and stirring was continued for 3 hours at room temperature. Use a centrifuge to separate the precipitate, wash the precipitate 3 times with deionized water and absolute ethanol, place it on a watch glass, and dry it in an oven at 50°C for 24 hours to obtain SiO 2 White powder.

[0026] 2.SiO 2 Surface activation of microspheres: the dried SiO 2 Place the microspheres in a 100ml beaker, add 25ml of nitric acid and 25ml of deionized mixed solution, ultrasonically disperse, cover with plastic wrap and soak for 24h. SiO after soaking 2 Wash with deionized water to neutral to obtain surface activated SiO 2 The microspheres...

Embodiment 2

[0032] 1. Same as Example 1.

[0033] 2. Same as Example 1.

[0034] 3. Same as Example 1.

[0035] 4. Same as Example 1.

[0036] 5. Same as Example 1.

[0037] 6. The elution of imprinted template molecules: using the method of ultrasonic extraction, the extraction solution is methanol solution, the methanol solution is changed every 30 minutes, the amount of methanol used each time is 100ml, and the total elution time is 300 minutes. After the extraction is completed, rinse with acetone for 1 Second, remove a small amount of fine polymer fragments. Dry under vacuum at 40°C for 24h.

Embodiment 3

[0039] 1. Same as Example 1.

[0040] 2. Same as Example 1.

[0041] 3. Same as Example 1.

[0042] 4. Same as Example 1.

[0043] 5. Preparation of L-carnitine molecularly imprinted microspheres with core-shell structure: Dissolve 1mmol (0.1612g) of template molecule L-carnitine in 20ml methanol, add 4mmol (0.314ml) methacrylic acid (MAA), at room temperature Oscillate at a constant temperature in the gas bath for 2 hours to make the template molecule and the functional monomer fully interact. Take 200mg SiO 2 -RAFT microspheres are dispersed in 50ml methanol solution, and then the methanol mixed solution of L-carnitine and MAA prepared before and 10mmol (1.93ml) crosslinker ethylene glycol dimethacrylate (EGDMA) are added to react The vessel was sealed, nitrogen was passed through to remove oxygen, 10ml of methanol solution containing 40mg of initiator azobisisobutyronitrile (AIBN) was added dropwise with a syringe, and the reaction was carried out at 50°C for 6 hours, then the te...

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Abstract

The invention discloses L-carnitine molecularly imprinted microspheres with a core-shell structure and a preparation method thereof, and relates to the field of intelligent high polymer materials. According to the invention, L-carnitine is taken as a template molecule and methacrylic acid is taken as a functional monomer, and the L-carnitine molecularly imprinted microspheres with the core-shell structure are prepared through reversible addition-fragmentation chain transfer and free radical polymerization in combination with the surface molecular imprinting technology; the preparation method of the L-carnitine molecularly imprinted microspheres with the core-shell structure comprises the following steps of: 1, preparing nano SiO2 microspheres by a Stober method; 2, performing surface activation on the nano SiO2 microspheres; 3, performing surface benzylation on the nano SiO2 microspheres; 4, synthesizing an RAFT (Reversible Addition-Fragmentation Chain Transfer Polymerization) reagent on the surface of the nano SiO2 microspheres; 5, preparing the L-carnitine molecularly imprinted microspheres with the core-shell structure; and 6, eluting the imprinted template molecules. The L-carnitine molecularly imprinted microspheres with the core-shell structure has the advantages of simple process, low cost, convenience in industrial popularization and application, and the like; the obtained imprinted microspheres have good adsorbability and selectivity for L-carnitine molecules, and can be applied to separating out carnitine enantiomers.

Description

Technical field [0001] The present invention relates to the field of intelligent polymer materials, in particular to a core-shell structure molecularly imprinted nano-microsphere with good adsorption and separation functions for L-carnitine molecules and a preparation method thereof. The nano-microsphere is through reversible addition- Fragmented chain transfer radical polymerization combined with surface molecular imprinting technology to prepare, can realize the selective recognition of L-carnitine. The molecularly imprinted polymer can be applied to the separation of carnitine enantiomers and other fields. Background technique [0002] Molecular imprinting technology is a rapidly developing technology in recent years for preparing molecularly imprinted polymers (MIP) with molecular recognition properties for specific target molecules. Due to the remarkable characteristics of pre-determination, selectivity and practicability, molecularly imprinted polymers have become one of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08F222/14C08F220/06C08F2/38C08J9/28C08J9/26C07C229/22C07C227/34
Inventor 陈娇娇付新蕾侯蔺桐傅宏俊程立伟许轩明
Owner TIANJIN POLYTECHNIC UNIV
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