A super-resolution fluorescence microscopy imaging device based on the photoabsorption modulation characteristics of azobenzene polymer

Abstract

The invention discloses a super-resolution fluorescence micro imaging device based on photoinduced absorption modulation characteristics of azobenzene polymer, a screw phase plate is utilized to shape a 355nm-wavelength laser into a hollow light beam; and the hollow light beam and a 532nm-wavelength light beam are combined and are simultaneously irradiated onto an azobenzene polymer film after being focused by a high-number-aperture oil immersion micro objective. The azobenzene film irradiated by the 355nm-wavelength laser has high absorptivity for the 532nm-wavelength laser; the 532nm-wavelength laser can penetrate through the azobenzene polymer film only at the middle hollow part of the 355nm-wavelength light beam, thereby exciting the fluorescence molecules on the film to emit light; and the fluorescence emitted by the sample is collected by the same objective and received by a detector. The hollow part after focusing the 355nm-wavelength light beam is subwavelength scale, and thus, can implement exciting light focused light spot beyond the diffraction limit on the detected sample; and thus, the fluorescence micro imaging of the whole sample beyond the diffraction limit can be implemented by point-by-point scanning.

Description

technical field [0001] The invention relates to the field of super-resolution optical imaging, in particular to a super-resolution fluorescence microscopic imaging device based on the photoabsorption modulation characteristics of azobenzene polymers. Background technique [0002] Microscopic technology is the most direct means for people to understand the microscopic world. Optical microscopy technology presents the image of the microscopic world directly in front of our eyes. It is the most intuitive and commonly used microscopic technology among all microscopic technologies. Restricted by the diffraction limit, the resolution can only reach the order of half a wavelength, which can no longer meet the resolution requirements of today's microscopes. In recent years, some super-resolution microscopy techniques have been proposed and matured. These super-resolution microscopy techniques mainly include confocal microscopy, scanning probe microscopy, stimulated emission fluoresc...

AI Technical Summary

Problems solved by technology

[0004] 2. The stability is not high

High-precision equipment such as near-field optical probes are easily damaged and need to be replaced regularly

[0005] 3. Limitations

The improvement of resolution by confocal microscopy is relatively limited; commonly used scanning probe microscopy techniques such as atomic force microscopy can only provide surface relief images of solid samples; stimulated emission fluorescence quenching microscopy techniques need to obtain high resolution Ultrashort pulse light source is required, and the pulse emission time should be precisely controlled

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