Quadruple-PCR (polymerase chain reaction) quick detection kit for main pathogenic bacteria of cow mastitis

Abstract

The invention discloses a quadruple-PCR (polymerase chain reaction) quick detection kit for main pathogenic bacteria of cow mastitis. The quick detection kit comprises a primer pair group comprising four pairs of specific primers. Specific genes sip, nuc, rrnB and HblA of pathogenic streptococcus agalactiae, staphylococcus aureus, escherichia coli and bacillus cereus can be amplified at the same time in one time in combination with a multi-PCR technology by the quick detection kit, and PCR amplification products are detected through agarose gel electrophoresis, so that the main pathogenic bacteria in a milk sample can be comprehensively, efficiently and accurately detected and identified. According to the quick detection kit, the shortcomings of cumbersomeness for operation, time consumption, manpower consumption and the like in a conventional pathogenic bacteria detection technology are overcome, the variety of pathogenic bacteria inspection is added, and the detection specificity and sensitivity are improved; the quick detection kit has the characteristics of better specificity and better sensitivity in comparison with a conventional detection method, is suitable for quick detection in grassroots veterinary station and cow breeding farms, and has a better application prospect.

Description

Technical field [0001] The invention belongs to the technical field of detecting pathogenic bacteria of dairy cow mastitis, and particularly relates to a four-fold PCR rapid detection kit for the main pathogenic bacteria of dairy cow mastitis. Background technique [0002] Rapid detection and identification of pathogens in milk cow milk is a prerequisite for timely and effective control and prevention of the spread of pathogens, which is of great significance to the health of dairy cows and humans. At present, the detection methods of pathogenic bacteria mainly rely on conventional bacteriological culture methods, which generally require 4 to 7 days, which is cumbersome and time-consuming. For pathogenic bacteria detection, the latex agglutination method, immunomagnetic bead separation method, enzyme immunoassay method, nucleic acid Hybridization technology and PCR technology are widely used because of their strong specificity, high sensitivity, simple operation, rapid detection ...

AI Technical Summary

Problems solved by technology

At present, the detection method of pathogenic bacteria mainly relies on the conventional bacteriological culture method, which generally takes 4-7 days, and the operation is cumbersome, time-consuming and labor-intensive; the latex agglutination method, immunomagnetic bead separation method, enzyme immunoassay method, nucleic acid Hybridization technology and PCR technology are widely used because of their strong specificity, high sensitivity, simple operation, and rapid detection, but these methods usually only detect one pathogenic microorganism in each experiment

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