Polyketone compound, preparation method and application thereof
A technology of polyketide compounds and uses, which is applied in the biological field and can solve problems such as no herbicidal activity
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Embodiment 1
[0033] Embodiment 1, bacterial strain screening:
[0034] In November 2012, healthy katydid samples were captured from the suburbs of Jinhua, and the samples were brought back to the laboratory for processing as soon as possible.
[0035] The medium is MEA medium: 20g of malt extract, 20g of sucrose, 20g of agar, 1g of peptone, distilled water to 1L, pH 7.0, 1.1 atmospheric pressure, sterilized at 121°C for 20min (for conventional sterilization).
[0036] Methods: Healthy katydid insects were starved for 24 hours and disinfected with 75% (volume %) alcohol for 2 minutes under aseptic conditions, rinsed with sterile water for 3 times, and then dissected. Take the intestinal tract and add a small amount of sterile water to grind it in a mortar to obtain a mixture. Suspension, respectively diluted with sterile water for 10 1 、10 2 、10 3 times, take 0.2 mL of each gradient dilution and spread it on the MEA medium plate, and culture it in a 28°C incubator for 2-3 days. After th...
Embodiment 2
[0037] Embodiment 2, bacterial strain identification
[0038] ITS rDNA analysis:
[0039] According to the instructions of BioTeke's new rapid plant genomic DNA extraction kit, the genomic DNA of the symbiotic bacteria ZS07 obtained in the above-mentioned Example 1 was extracted, using ITS universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3'forward) and ITS4 (5'-TCCTCCGCTTATTGATATGC-3 ', reverse) to amplify the rDNA ITS region gene sequence and purify it.
[0040] The similarity of the sequencing results was compared with the BLAST program; the analysis results showed that the symbiotic fungus ZS07 had a high similarity with the strain F. Proliferatum (FJ 648201), with a similarity of 99.5%. Combined with the morphological characteristics of the bacterium, the bacterium was identified as F. Proliferatum.
[0041] The bacterium has been preserved, the preservation unit: China Type Culture Collection Center; the preservation name is: Fusarium proliferatum ZS07 (Fusarium prolifera...
Embodiment 3
[0042] Embodiment 3, the preparation of polyketide compound
[0043] The mycelium block (0.5 × 0.5cm) of fresh Fusarium proliferatum ZS07 (Fusarium proliferatum ZS07) 2 , about 0.5 g) was inoculated into 250 mL Erlenmeyer flasks containing 150 mL of ME medium in each bottle, inoculated into 5 to 6 bottles, and cultivated at 180 rpm and 28° C. for 2 to 3 days as a seed solution.
[0044] Remarks: Fresh Fusarium ZS07 can be obtained after activation of Fusarium ZS07 by conventional malt solid medium (MEA medium).
[0045] 10 mL of seed solution was inoculated into a 1000 mL Erlenmeyer flask containing 400 mL of ME medium, and fermented for 7 days at 180 rpm and 28°C.
[0046] The fermentation broth was filtered with two layers of gauze, the filtrate was extracted with an equal volume of ethyl acetate, the number of extractions was 3 times, concentrated and dried in vacuo (at a vacuum degree of 0.1 negative pressure, dried at 45°C for 30-50 minutes), and the crude extract 40.5 g....
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