Method for producing debaromyces hansenii for feeds by fermenting high-salt-content amino acid wastewater
An amino acid, high-salt technology, applied in the field of Debaryomyces hansenii, can solve the problems of unsuitable treatment of amino acid wastewater, high cost of concentration treatment process, high investment and operation cost, and achieve high nitrogen source utilization efficiency and high application. The effect of promotion potential and strong competitive advantage
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Embodiment 1
[0025] Example 1: Acclimatization and cultivation of Debaryomyces hansenii strains for feed.
[0026] 1. Take the waste liquid from an amino acid factory in Wuhan, after the hydrolysis of hair acid to produce cysteine, and use it as the high-salt amino acid wastewater required below. Its composition table is as follows:
[0027] Table 1 Contents of related elements in amino acid wastewater
[0028] project name
unit
Test results
Method basis
Total Nitrogen (N)
%
8.79
GB / T8572-2010
Sodium (Na)
%
0.52
ICP method
Chlorine (Cl)
g / L
131.26
AgCl nephelometric method
Lead (Pb)
mg / kg
0.91
ICP method
Arsenic (As)
mg / kg
0.076
Mercury (Hg)
mg / kg
0.014
Chromium (Cr)
mg / kg
1.51
ICP method
Cadmium (Cd)
mg / kg
0.06
ICP method
[0029] Table 2 Amino acid composition and cont...
Embodiment 2
[0037] Fermentation of Debaryomyces hansenii in liquid medium with glucose as carbon source and waste water with different concentrations of amino acids as nitrogen source.
[0038] 1. Prepare two kinds of liquid fermentation medium for high salinity amino acid wastewater with different concentrations. Liquid fermentation media with high salinity amino acid wastewater concentration of 165g / L and 330g / L were prepared respectively, wherein the high salinity amino acid wastewater was the only nitrogen source (its source and composition were consistent with Example 1), and the liquid fermentation culture The base also adds other chemical substrates required by microorganisms, and its components are: glucose: 20 and 40g / L, KH 2 PO 4 : 7.5 and 15g / L, yeast powder: 7.5 and 15g / L, MgSO 4 : 4.5 and 9g / L, CaCO 3 : 1 and 2g / L, FeSO 4 : 2.5 and 5g / L, the pH of the medium is 5.5. The two culture media were coded as No. 1 and No. 2, respectively.
[0039] 2. Inoculate the domesticated...
Embodiment 3
[0043] Fermentation of Debaryomyces hansenii in a liquid medium with enzymatic hydrolyzate of corn starch as carbon source and waste water with different concentrations of amino acids as nitrogen source.
[0044] 1. Prepare two kinds of liquid fermentation medium for high salinity amino acid wastewater with different concentrations. Liquid fermentation media with high salinity amino acid wastewater concentration of 165g / L and 330g / L were prepared respectively, wherein the high salinity amino acid wastewater was the only nitrogen source (its source and composition were consistent with Example 1), and the liquid fermentation culture The base also adds other chemical substrates required by microorganisms, and its components are: corn starch enzymatic hydrolysis solution (according to the dry weight of corn starch) 60 and 90g / L, KH 2 PO 4 : 7.5 and 15g / L, yeast powder 0 and 15g / L, MgSO 4 : 4.5 and 9g / L, CaCO 3 : 1 and 2g / L, FeSO 4 : 2.5 and 5g / L, the pH of the medium is 5.5. ...
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