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A kind of Clostridium butyricum living bacteria preparation processing method and culture medium composition
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A technology for Clostridium butyricum and viable bacteria preparations, applied in the biological field, can solve the problems of low spore rate of fermentation broth, long time required for fermentation, etc., achieve short fermentation culture time, save energy consumption, and optimize the collection method of bacterial cells Effect
Active Publication Date: 2016-08-31
GUANGDONG HINAPHARM PHARMA CO LTD
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Problems solved by technology
[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a processing method and a culture medium composition of live Clostridium butyricum preparations, aiming at solving the needs of fermentation in the preparation process of the existing live Clostridium butyricum preparations. The time is very long, or the spore rate in the fermentation broth is very low
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Embodiment 1
[0076] 1. Activation and expansion of cultured strains:
[0077] 1) Activated strains: Streak the strains of Clostridium butyricum on the RCM solid medium and purify them. The RCM solid medium (clostridium enhanced proliferation medium) is produced by Qingdao Haibo Biotechnology Co., Ltd., and its components are: tryptone 10g / L, beef powder 10g / L, yeastpowder 3g / L, glucose 5g / L L, soluble starch 1g / L, sodiumchloride 5g / L, sodium acetate 3g / L, L-cysteine hydrochloride 0.5g / L, agar powder 20g / L, pH6.8-7.0.
[0078] 2) Expansion of cultured strains: Select a single colony on the RCM solid medium, inoculate it in 200 ml of RCM liquid medium, and place it in an anaerobic bag for anaerobic culture at 37°C for 48 hours. The RCM liquid medium is the same as above, but no agar is added.
[0079] 2. Seed tank culture strains:
[0080] Insert 200 milliliters of RCM liquid medium into the 50-liter first-level seed tank, use nitrogen to pass into the tank from the top of the tank, k...
Embodiment 2
[0089] 1. Activation and expansion of cultured strains:
[0090] 1) Activated strains: Streak the strains of Clostridium butyricum on the RCM solid medium and purify them. The RCM solid medium (clostridium enhanced proliferation medium) is produced by Qingdao Haibo Biotechnology Co., Ltd., and its components are: tryptone 10g / L, beef powder 10g / L, yeast powder 3g / L, glucose 5g / L L, soluble starch 1g / L, sodium chloride 5g / L, sodium acetate 3g / L, L-cysteine hydrochloride 0.5g / L, agar powder 20g / L, pH6.8-7.0.
[0091] 2) Expansion of cultured strains: Select a single colony on the RCM solid medium, inoculate it in 200 ml of RCM liquid medium, and place it in an anaerobic bag for anaerobic culture at 37°C for 48 hours. The RCM liquid medium is the same as above, but no agar is added.
[0092] 2. Seed tank culture strains:
[0093] Insert 200 milliliters of RCM liquid medium into the 50-liter first-level seed tank, use nitrogen to pass into the tank from the top of the tank, k...
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Abstract
The invention discloses a processing method of a viable clostridium butyricum preparation and a culture medium composition. The processing method comprises the following steps: carrying out activation and enlarge cultivation on strains; culturing the strains by utilizing a seeding tank; carrying out fermentation cultivation by utilizing a fermentation tank, wherein a fermentation culture medium comprises the following components: corn flour, peptone, beef extractpowder, yeast extracts, dipotassium phosphate, monopotassium phosphate, calciumchloride, magnesiumsulfate, manganesesulfate, ferroussulfate, sodium acetate and cysteine salt. According to the processing method, by optimizing the culture medium and a processing process, the fermentation cultivation is short, and the maturing rate of spores is high; furthermore, a thallus collection method is further optimized, so that the production process is greatly simplified, the energy consumption can be substantially reduced, and the yield of active thalli can be improved.
Description
technical field [0001] The invention relates to the field of biological technology, in particular to a processing method and a culture medium composition of live Clostridium butyricum preparations. Background technique [0002] Clostridium butyricum is one of the genusClostridium, which mainly exists in cheese, natural yogurt, human and animal intestines and feces, certain leaves, soil and other natural environments. Clostridium butyricum is a straight or curved Gram-positive anaerobic endophytic bacillus with some resistance to environmental changes, these characteristics make C. butyricum a good probiotic potential. On the other hand, Clostridium butyricum is resistant to gastric acid and bile salts when it passes through the intestinal tract of animals, which ensures its performance. It is widely used in intestinal medicine, health food, feed additive, microbial fertilizer, etc. in clinical medicine and animal husbandry. It is an ideal biological preparation with broad ...
Claims
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