An oblique wide-field optical section scanning imaging microscope system and its imaging method

Abstract

The invention discloses an inclined wide-field optical section scanning imaging microscope system and an imaging method thereof. The inclined wide-field optical section scanning imaging microscope system comprises a laser transmitting device, wherein laser transmitted by the laser transmitting device enters a laser scanning optical path consisting of a two-dimensional scanning galvanometer, a collimator lens group and a first microscope objective to perform inclined scanning on a sample on a sample platform; a second microscope objective, a field lens and a detector form an imaging detection optical path the optical axis of which is perpendicular to the laser scanning optical path; an imaging display control device respectively controls the synchronized action of the two-dimensional scanning galvanometer, the second microscope objective and the detector and the automatic displacement of the sample platform through a data acquisition card and a sample platform control device, and processes acquired imaging data of the detector to form wide field three-dimensional image information of the sample. The imaging microscope system and the imaging method disclosed by the invention have high scanning imaging speed and high resolution and can implement wide field scanning imaging.

Description

technical field [0001] The invention belongs to the field of optical microscopy, in particular to an optical section illumination scanning microscopic imaging device and a microscopic imaging method. Background technique [0002] With the development of science and technology, people have put forward higher and higher requirements for the resolution of microscopic structures and microscopic imaging of biological functions. In addition to the requirement of high resolution, the detection time and detection range also need to be faster and wider. [0003] In 1994, light section illumination fluorescence microscopy, that is, planar light microscopy, was first proposed and used to observe larger samples, initially using orthogonal light section illumination microscopy. In this design, a light section is used to illuminate the sample vertically, and then a fluorescent image of the illuminated surface is observed in a direction perpendicular to the illuminating light. However, d...

AI Technical Summary

Problems solved by technology

However, during the scanning process of this kind of optical slice, the scanning process needs to be realized by moving the platform, and the scanning speed is relatively slow.

[0004] However, at present, in many fields of biology and medicine, it is often necessary to quickly perform microscopic imaging of a wide range of biological tissues while ensuring high resolution, and the existing technologies still cannot meet this requirement.

Images