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Method for preparing heparin sodium by utilizing small intestines of pigs

A technology for porcine small intestine and heparin sodium, which is applied in the field of biomedicine, can solve the problems of unstable product quality of heparin sodium, low yield of heparin sodium, backward production process, etc., so as to solve the problem of odor in workshop, high yield, potency and activity. improved effect

Inactive Publication Date: 2014-04-30
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional production process is backward, the yield of heparin sodium is low, and the quality of heparin sodium product is unstable.
[0003] The waste of resources in the production process is serious and the environment is seriously polluted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation method of producing heparin sodium by utilizing pig small intestine adopts the following steps:

[0035] (1) Preparation of intestinal mucosa:

[0036] Take 400 pig small intestines and wash them with water to remove residual feces and clean them; fill the intestines with clean water, and use a casing machine to squeeze out the small intestinal mucosa. The operation is uniform, and the mucosa enters the mucosa pool along the mucosal pipeline; The head and broken intestinal skin were minced and then put into the mucous membrane pool, and a total of 420 kg of mucous membrane was collected;

[0037] (2) Enzymolysis:

[0038] Turn on the mucous membrane pump, pump 420 kg of mucous membrane from the mucous membrane pool into the enzymolysis tank, add 3360 kg of water into the enzymolysis tank, turn on the mixer to stir at a speed of 50 r / min, stir evenly, and then add 840 grams of 2709 alkaline protease Add it into the enzymolysis tank, adjust the pH value ...

Embodiment 2

[0051] The preparation method of producing heparin sodium by utilizing pig small intestine adopts the following steps:

[0052] (1) Preparation of intestinal mucosa:

[0053] Take 300 pig small intestines and wash them with water to remove residual feces and clean them; fill the intestines with clean water, and use a casing machine to squeeze out the small intestinal mucosa. The operation is even and the mucosa enters the mucosa pool along the mucosal pipeline; The head and broken intestinal skin were minced and then put into the mucous membrane pool, and a total of 310 kg of mucous membrane was collected;

[0054] (2) Enzymolysis:

[0055] Turn on the mucous membrane pump, put 310 kg of mucous membrane from the mucous membrane pool into the enzymolysis tank, add 2790 kg of water into the enzymolysis tank, turn on the mixer and stir at a speed of 55 r / min, stir evenly, then add 620 grams of 2709 alkaline protease Add it into the enzymolysis tank, adjust the pH value to 8.0; op...

Embodiment 3

[0068] The preparation method of producing heparin sodium by utilizing pig small intestine adopts the following steps:

[0069] (1) Preparation of intestinal mucosa:

[0070] Take 500 pig small intestines and wash them with water to remove residual feces and clean them; fill the intestines with clean water, and use a casing machine to squeeze out the small intestinal mucosa. The operation is even and the mucosa enters the mucosa pool along the mucosal pipeline; The head and broken intestinal skin were minced and then put into the mucous membrane pool, and a total of 50 kg of mucous membrane was collected;

[0071] (2) Enzymolysis:

[0072] Turn on the mucous membrane pump, pump 520 kg of mucous membrane from the mucous membrane pool into the enzymolysis tank, add 3640 kg of water into the enzymolysis tank, turn on the mixer to stir at a speed of 60 r / min, stir evenly, and then add 1040 grams of 2709 alkaline protease Add it into the enzymolysis tank, adjust the pH value to 9...

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Abstract

The invention discloses a preparation method for biochemical medicine separated from mucous membrane of small intestines of pigs, and especially relates to a method for preparing heparin sodium by utilizing small intestines of pigs. The method for preparing heparin sodium by utilizing small intestines of pigs is characterized by comprising the following steps: preparing intestinal mucosa, performing enzyme digestion, adsorption, eluting, precipitation desalting and dewatering drying to obtain heparin sodium. The beneficial effects comprise that the yield is high, the heparin sodium activity loss is small, the quality is stable, waste liquid is discharged without pollution and can be recycled, by employing a workshop waste-gas purifying processing apparatus, the problem of foul odor in a heparin sodium workshop is solved; and 2000 to 2400 pieces of pig small intestines are needed for producing 0.1 billion units of heparin sodium by using the conventional process, only 1400 to 1600 pieces of pig small intestines are needed for producing 0.1 billion units of heparin sodium by using the preparation method, and the effect and the activity of the heparin sodium are greatly improved, so that the production cost is greatly reduced, and the profit can be improved by 40% or more.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing heparin sodium from pig small intestine. Background technique [0002] Heparin is a mucopolysaccharide that widely exists in mammalian tissues, mainly exists in mast cells, has anticoagulant effect, and is widely used in the treatment of surgery, cerebral thrombosis, myocardial infarction, etc. The molecular weight of heparin is about 3000-37500, and research on commercial heparin suggests that there are at least 21 molecular entities. Heparin sodium is the sodium salt of heparin. As a natural anticoagulant substance, heparin sodium has been valued by countries all over the world. It is also one of the main export drugs in my country. With the deepening of research, people found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid regulation functions, but also has anti-inflammatory, anti-allergic, anti-viral, anti-cancer and other fu...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 刘乃山尚明彩迟培升夏衬来
Owner QINGDAO JIULONG BIO PHARMA
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