Method for preparing heparin sodium by utilizing small intestines of pigs

A technology for porcine small intestine and heparin sodium, which is applied in the field of biomedicine, can solve the problems of unstable product quality of heparin sodium, low yield of heparin sodium, backward production process, etc., so as to solve the problem of odor in workshop, high yield, potency and activity. improved effect

Inactive Publication Date: 2014-04-30
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional production process is backward, the yield of heparin sodium is low, and the quality of heparin sodium p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1:

[0034] The preparation method of using pig small intestine to produce heparin sodium adopts the following steps:

[0035] (1) Preparation of intestinal mucosa:

[0036] Take 400 pig small intestines and wash them with water to remove residual feces and clean them; pour clean water into the intestines, squeeze out the small intestine mucosa with a casing machine, operate with uniform force, the mucosa will enter the mucosal pool along the mucosal duct; the intestines in the scraping process The head and intestines were minced and then thrown into the mucosal pool to collect a total of 420 kg of mucosa;

[0037] (2) Enzymatic hydrolysis:

[0038] Turn on the mucosal pump, pump 420 kg of mucosa from the mucosal pool into the enzymatic hydrolysis tank, add 3360 kg of water into the enzymatic hydrolysis tank, turn on the mixer and stir at a speed of 50r / min, stir evenly, and then add 840 g of 2709 alkaline protease Add to the enzymolysis tank and adjust the pH to 8.0; o...

Example Embodiment

[0050] Example 2:

[0051] The preparation method of using pig small intestine to produce heparin sodium adopts the following steps:

[0052] (1) Preparation of intestinal mucosa:

[0053] Take 300 pig small intestines and wash them with water to remove residual feces and clean them; pour clean water into the intestines, squeeze out the small intestinal mucosa with a casing machine, operate with even force, the mucosa will enter the mucosal pool along the mucosal duct; the intestines in the scraping process The head and intestines were minced and then thrown into the mucosal pool to collect 310 kg of mucosa;

[0054] (2) Enzymatic hydrolysis:

[0055] Turn on the mucosal pump, pump 310 kilograms of mucosa from the mucosal pool into the enzymolysis tank, add 2790 kilograms of water into the enzymolysis tank, turn on the mixer and stir at a speed of 55r / min, stir evenly, and then add 620 grams of 2709 alkaline protease Add to the enzymolysis tank and adjust the pH to 8.0; open the steam...

Example Embodiment

[0067] Example 3:

[0068] The preparation method of using pig small intestine to produce heparin sodium adopts the following steps:

[0069] (1) Preparation of intestinal mucosa:

[0070] Take 500 pig small intestines and wash them with water to remove residual feces and clean them; pour clean water into the intestines, squeeze out the small intestinal mucosa with a casing machine, operate with uniform force, the mucosa will enter the mucosal pool along the mucosal duct; the intestines during scraping The head and intestines are minced and then thrown into the mucosal pool to collect a total of 50 kg of mucosa;

[0071] (2) Enzymatic hydrolysis:

[0072] Turn on the mucosal pump, pump 520 kg of mucosa from the mucosal pool into the enzymatic hydrolysis tank, add 3640 kg of water into the enzymatic hydrolysis tank, turn on the mixer to stir, rotate at 60r / min, stir evenly, and then add 1040 g of 2709 alkaline protease Add to the enzymolysis tank, adjust the pH to 9.0; open the steam v...

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Abstract

The invention discloses a preparation method for biochemical medicine separated from mucous membrane of small intestines of pigs, and especially relates to a method for preparing heparin sodium by utilizing small intestines of pigs. The method for preparing heparin sodium by utilizing small intestines of pigs is characterized by comprising the following steps: preparing intestinal mucosa, performing enzyme digestion, adsorption, eluting, precipitation desalting and dewatering drying to obtain heparin sodium. The beneficial effects comprise that the yield is high, the heparin sodium activity loss is small, the quality is stable, waste liquid is discharged without pollution and can be recycled, by employing a workshop waste-gas purifying processing apparatus, the problem of foul odor in a heparin sodium workshop is solved; and 2000 to 2400 pieces of pig small intestines are needed for producing 0.1 billion units of heparin sodium by using the conventional process, only 1400 to 1600 pieces of pig small intestines are needed for producing 0.1 billion units of heparin sodium by using the preparation method, and the effect and the activity of the heparin sodium are greatly improved, so that the production cost is greatly reduced, and the profit can be improved by 40% or more.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing heparin sodium from pig small intestine. Background technique [0002] Heparin is a mucopolysaccharide that widely exists in mammalian tissues, mainly exists in mast cells, has anticoagulant effect, and is widely used in the treatment of surgery, cerebral thrombosis, myocardial infarction, etc. The molecular weight of heparin is about 3000-37500, and research on commercial heparin suggests that there are at least 21 molecular entities. Heparin sodium is the sodium salt of heparin. As a natural anticoagulant substance, heparin sodium has been valued by countries all over the world. It is also one of the main export drugs in my country. With the deepening of research, people found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid regulation functions, but also has anti-inflammatory, anti-allergic, anti-viral, anti-cancer and other fu...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 刘乃山尚明彩迟培升夏衬来
Owner QINGDAO JIULONG BIO PHARMA
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