Preparation method of yoghourt
A technology for yogurt and milk volume, applied in milk preparations, dairy products, applications, etc., can solve the problems of insufficient nutritional value and health care function of yogurt, unremarkable typical flavor of fermented milk, poor sensory quality of yogurt, etc., and achieve taste Small changes in whey precipitation, shortened coagulation time, sour taste and refreshing effect
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Embodiment 1
[0035] The concrete breeding process of described plantarum lactobacillus is as follows:
[0036] 1. Diethyl Sulfate (DES) Mutation Breeding
[0037] (1) Take a ring of Lactobacillus plantarum on the inclined surface of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask containing 50mL liquid MRS xylan medium, and incubate at 200rpm at 40°C for about 12h, so that the bacteria are in the right condition. Early growth period.
[0038] (2) Take 5mL of bacterial liquid, centrifuge at 5000rpm for 10min to collect bacterial cells, and wash twice with normal saline.
[0039] (3) Dilute to 10 with pH7.0 phosphate buffer 7 individual / mL bacterial suspension.
[0040] (4) Take 32mL of pH7.0 potassium phosphate buffer, 8mL of bacterial suspension, and 0.4mL of DES in a 150mL Erlenmeyer flask pre-placed in the rotor and mix thoroughly so that the final concentration of DES is 1% (v / v).
[0041] (5) React in a shaker at 30°C at 150rpm for 30min, take 1mL of...
Embodiment 2
[0057] A method for preparing yogurt, comprising the steps of:
[0058] Raw material processing: acceptance, sterilization, storage, standardization, batching, degassing and homogenization of raw milk;
[0059] Secondary sterilization: After homogenization, the material is sterilized through a heat exchanger under the condition of 95°C for 300s;
[0060] Cooling and inoculation: the sterilized material is cooled to 44°C, and the mixed working fermentation bacteria agent is added in an amount of 3% of the original milk.
[0061] The mixed working starter is composed of Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animalis;
[0062] The mass ratio of the Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animalis is 1:0.2:0.2;
[0063] Fermentation: After the inoculation work is completed, start stirring, stop stirring after 15 minutes, keep the temperature of the fermenter at 43°C, ferment for 3 hours, and stop the fermentation when...
Embodiment 3
[0066] A method for preparing yogurt, comprising the steps of:
[0067] Raw material processing: acceptance, sterilization, storage, standardization, batching, degassing and homogenization of raw milk;
[0068] Secondary sterilization: After homogenization, the material is sterilized through a heat exchanger under the condition of 95°C for 300s;
[0069] Cooling and inoculation: the sterilized material is cooled to 44°C, and the mixed working fermentation bacteria agent is added in an amount of 4% of the original milk.
[0070] The mixed working starter is composed of Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animalis;
[0071] The mass ratio of the Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animalis is 1:0.4:0.3;
[0072] Fermentation: After the inoculation work is completed, start stirring, stop stirring after 15 minutes, keep the temperature of the fermenter at 45°C, ferment for 2.5 hours, and stop the fermentation wh...
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