Method for curing loose coral sand by utilizing microorganisms

A technology of coral sand and microorganisms, applied in the field of chemistry, can solve problems such as no one cares about, and achieve the effects of solving high transportation costs, improving reproductive capacity, and being convenient to carry

Active Publication Date: 2014-05-07
LOGISTICAL ENGINEERING UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some progress has been made in the microbial treatment of ordinary sand (silica sand), but no one cares about the microbial treatment of coral sand (calcareous sand) whose main component is calcium carbonate; The particularity of the environment, optimize the cultivation and preservation of biological bacteria, improve their activity in high-salinity seawater, and ensure the smooth progress of microbial reactions in seawater

Method used

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  • Method for curing loose coral sand by utilizing microorganisms
  • Method for curing loose coral sand by utilizing microorganisms
  • Method for curing loose coral sand by utilizing microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Acclimatization, cultivation and preservation of bacteria

[0029] The culture medium was prepared, the composition of the culture medium was shown in Table 1, and the solvent was water.

[0030] Table 1 Medium composition

[0031] Element

Yeast extract

urea

Sodium chloride

Nickel chloride (hexahydrate)

Manganese chloride (tetrahydrate)

content

20g / L

20g / L

10 / 20 / 30 / 35g / L

23.8mg / L

13.9mg / L

[0032] Inoculate Bacillus pasteurii into the medium prepared according to Table 1 (in which the sodium chloride content is 10g / L), and culture at 25±2°C for 24h; then inoculate until the sodium chloride is 20g / L medium for 24 hours, and then in the medium with a sodium chloride concentration of 30g / L and 35g / L for 24 hours, until the activity of the bacterial liquid is >2mM·min under the condition of a sodium chloride concentration of 35g / L -1 , that is, stop the acclimatization culture; then centrifuge the bacteria...

Embodiment 2

[0035] Solidification of coral sand

[0036] Coral sand is a special type of rock-soil medium developed in tropical marine environments. It is mainly composed of coral debris and other marine biological debris, with high calcium carbonate content. The SEM scanning photos of coral sand are as follows: figure 1 shown.

[0037] The solidification method of coral sand: the calcium chloride solution that concentration is 2mol / L and the urea solution of 2mol / L are mixed in equal volume, then the bacterium liquid that embodiment 1 makes and the mixed solution containing calcium chloride and urea press 1 : 2 volume ratio mixed to get a mixed solution, and then inject the mixed solution into the coral sand from the upper part, the injection amount is equivalent to 1.5 times of the pore volume of the coral sand, and naturally discharge the liquid from the lower part, and control the outflow rate to 2-10mL / min, injected once a day for 3-6 consecutive days. Due to the consideration of ...

Embodiment 3

[0040] strength test

[0041] The unconfined compressive strength test was carried out on the cured coral sand column. Before the test, the surface of the sand column was smoothed, and dried in an oven at 50°C for 48 hours, and then the test was carried out. The stress-strain curve measured by the unconfined compressive strength test is as follows: image 3 As shown, the failure mode is as Figure 4 As shown, the test results are shown in Table 2. It can be seen from Table 2 that the unconfined compressive strength of the solidified coral sand column can reach up to about 14MPa.

[0042] Table 2. Compressive strength of coral sand column samples

[0043] sand column

diameter / cm

Height / cm

Compressive strength / MPa

Way of destruction

1

5.141

8.385

5.76

Fracturing damage

2

5.145

7.763

6.68

Fracturing damage

3

5.115

8.505

7.70

Fracturing damage

4

5.120

8.815

14.14

...

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Abstract

The invention provides a method for curing loose coral sand by utilizing microorganisms. The method comprises the following steps: acclimating bacillus pasteurii under the condition that the concentration of sodium chloride is 10-35g/L, and when the activity of lysate is more than 2mM.min<-1> under the condition the concentration of sodium chloride is 35g/L, centrifugally collecting thallus and adopting a vacuum freezing and drying method for cold storage; then adding preserved thallus into seawater to prepare into the lysate, adjusting pH to 7-8, then mixing the lysate and a solution each liter of which contains 1mol of calcium and 1mol of urea according to the volume ratio of 1:2, filling the mixture into coral sand once every day and 3-6 days in total. The method provided by the invention has the advantages that the particles are meshed by wrapped carbon calcium, so that the strength is up to 14MPa; and the thallus can be directly diluted by using seawater, and the solution with calcium chloride and urea can be directly prepared by using seawater, so that the problems of shortage of fresh water and material transportation on reef islands are solved.

Description

technical field [0001] The invention belongs to the field of chemistry, in particular to a method for solidifying loose coral sand by using microorganisms. Background technique [0002] In the 21st century, mankind has entered a period of large-scale development and utilization of the ocean. With the gradual implementation of China's major deployment of "improving the development of marine resources, developing the marine economy, protecting the marine ecological environment, resolutely safeguarding the national marine rights and interests, and building a marine power", the construction of large-scale island and reef projects is imperative and urgent. For the construction of islands and reefs far away from the land, there is a lack of building materials, and all kinds of building materials required need to be transported from land, and the cost is very expensive. On the premise of not destroying the ecological environment of islands and reefs, the development of building ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/00C09K103/00
Inventor 方祥位楚剑申春妮吴仕帆李依珊
Owner LOGISTICAL ENGINEERING UNIVERSITY OF PLA
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