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Bacterial cellulose fermentation medium and method for producing bacterial cellulose by utilizing medium

A technology of bacterial cellulose and fermentation medium, applied in the biological field, can solve the problems of low output and yield and high cost of bacterial cellulose medium, achieve the effects of increasing added value, saving preparation costs and solving environmental pollution

Active Publication Date: 2014-05-07
陕西启悦医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problems of high cost, low yield and yield of bacterial cellulose medium are the bottlenecks of its industrial production and popularization and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The bacterial strain described in the present invention: Gluconacetobacter xylinus, purchased from China Agricultural Microorganism Culture Collection and Management Center, strain number accc10215.

[0024] A method for producing bacterial cellulose, comprising the steps of:

[0025] (1) Activation of Gluconacetobacter xylosus

[0026] Prepare gluconacetobacter xylosus activation medium, the formula of activation medium includes sucrose 40g / L, beef extract 10g / L, disodium hydrogen phosphate 4g / L, citric acid 0.8g / L, ethanol 8g / L, agar 15g / L, adjust the pH value to 5.5. Inoculate Gluconacetobacter xylosus accc10215 in the activation medium, culture at 28°C for 28 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 28°C for 28 hours to obtain activated wood Gluconacetobacter sucrose;

[0027] (2) Expansion of Gluconacetobacter xylosus

[0028] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expa...

Embodiment 2

[0037] A method for producing bacterial cellulose, comprising the steps of:

[0038] (1) Activation of Gluconacetobacter xylosus

[0039] Prepare gluconacetobacter xylosus activation medium, the formula of activation medium comprises sucrose 45g / L, beef extract 13g / L, disodium hydrogen phosphate 4.5g / L, citric acid 0.9g / L, ethanol 9g / L, Agar 17g / L, adjust the pH value to 6.0. Inoculate Gluconacetobacter xylosus accc10215 in the activation medium, culture at 28°C for 28 hours, re-inoculate the cultivated strains in the same, new activation medium, and cultivate at 28°C for 28 hours to obtain activated wood Gluconacetobacter sucrose;

[0040](2) Expansion of Gluconacetobacter xylosus

[0041] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expansion culture medium comprises sucrose 45g / L, beef extract 13g / L, disodium hydrogen phosphate 4.5g / L, citric acid 0.9g / L, ethanol 9g / L L, adjusting the pH value to 6.0, inoculating activated Gluconacetobacter...

Embodiment 3

[0050] A method for producing bacterial cellulose, comprising the steps of:

[0051] (1) Activation of Gluconacetobacter xylosus

[0052] Prepare Gluconacetobacter xylosus activation medium, the formula of activation medium includes sucrose 50g / L, beef extract 15g / L, disodium hydrogen phosphate 5g / L, citric acid 1.0g / L, ethanol 10g / L, agar 20g / L, adjust the pH value to 6.5. Inoculate Gluconoacetobacter xylosus accc10215 in the activation medium, culture at 32°C for 32 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 32°C for 32 hours to obtain activated wood Gluconacetobacter sucrose;

[0053] (2) Expansion of Gluconacetobacter xylosus

[0054] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expansion culture medium includes sucrose 50g / L, beef extract 15g / L, disodium hydrogen phosphate 5g / L, citric acid 1.0g / L, ethanol 10g / L , adjust the pH value to 6.5, inoculate the activated gluconacetobacter x...

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Abstract

The invention discloses a bacterial cellulose fermentation medium and a method for producing bacterial cellulose by utilizing the medium, belonging to the technical field of biology. Every liter of the bacterial cellulose fermentation medium comprises 25-35g of cane sugar, 4-5g of beef extract, 4-5g of disodium hydrogen phosphate, 0.8-1.0g of citric acid, 8-10g of ethanol and the balance of hydrolysate of distillers' grains, wherein the pH value of the fermentation medium is 5.5-6.5. The method for producing the bacterial cellulose by utilizing the medium comprises the following steps: (1) activating and propagating gluconacetobacter xylinus to obtain a gluconacetobacter xylinus seed solution, inoculating the gluconacetobacter xylinus seed solution into the bacterial cellulose fermentation medium for fermentation, thus obtaining bacterial cellulose fermentation liquor; and (2) treating a bacterial cellulose membrane in the bacterial cellulose fermentation liquor to obtain the bacterial cellulose product. The method has the advantages of low cost, simple process, convenient industrial production and the like, the stability and reproducibility are high, the yield of the bacterial cellulose obtained through fermentation is more than 10g / L, and the method is suitable for large-scale production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing bacterial cellulose by fermenting distiller's grains. Background technique [0002] Bacterial cellulose is produced by bacteria growing in a liquid sugar-containing matrix and secreted into the matrix. It is a high molecular polymer composed of glucose linked by β-1,4 glycosidic bonds. It is used in biomedicine Materials, pharmaceutical materials, pervaporation membranes, fuel cells, papermaking and its cellulose derivatives have broad application prospects. However, the problems of high cost, low yield and yield of bacterial cellulose medium are the bottlenecks of its industrial production and popularization and application. Utilizing various waste residues and waste liquids to produce bacterial cellulose and reduce its production cost is an effective way to promote its industrial production and application. Contents of the invention [0003] T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02
Inventor 张雯张敏娟李彦军刘兰张艳
Owner 陕西启悦医疗科技有限公司
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