Triple detection kit for human herpes viruses HSV-1, HSV-2 and HCMV

Abstract

The invention provides a triple detection kit for human herpes viruses HSV-1, HSV-2 and HCMV. The triple detection kit is composed of a quantitative PCR (Polymerase Chain Reaction) reaction liquid, an HSV-1 standard substance, an HSV-2 standard substance, an HCMV standard substance, a negative reference substance, an HSV-1 positive reference substance, an HSV-2 positive reference substance, an HCMV positive reference substance, a specification and a box, wherein the quantitative PCR reaction liquid contains a PCR buffer solution, MgC12, dNTPs (deoxyriboNucleoside Tri-Phosphates), a heat-resisting DNA polymerase, an upstream amplification primer, a downstream amplification primer, an HSV-1 fluorescent probe, an HSV-2 fluorescent probe and an HCMV fluorescent probe. The kit is capable of realizing simultaneous typing detection on the common human herpes viruses HSV-1, HSV-2 and HCMV in a sample by virtue of the real-time quantitative PCR technique and the specific fluorescent probes of three colors; the kit is capable of quantifying positive viruses accurately in real time, so that the production cost and the detection cost can be saved and the detection efficiency can be improved; besides, the kit is capable of meeting diagnosis requirements and helpful for formulating a targeting treatment scheme timely.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a fluorescent quantitative PCR detection kit, in particular to a three-probe real-time fluorescent quantitative PCR for detecting herpes simplex virus type 1 (HSV-1) and herpes simplex in samples such as blood, urine, and cerebrospinal fluid of a patient. New kits for three common human herpesviruses, virus type 2 (HSV-2) and human cytomegalovirus (HCMV), can simultaneously perform typing and quantitative detection of HSV-1, HSV-2 and HCMV. Background technique [0002] Herpesviridae is a group of medium-sized, enveloped DNA viruses, more than 100 species have been discovered. Herpesviruses known to be associated with human disease include herpes simplex virus types 1 and 2 (HSV-1, HSV-2), varicella-zoster virus (VZV), Epstein-Marr virus (EBV), human giant Cytovirus (HCMV), human herpesvirus types 6, 7 and 8 (HHV-6, HHV-7, HHV-8), the most common clinical infections are HSV-1,...

AI Technical Summary

Problems solved by technology

[0003] Traditional herpes virus detection methods mainly include virus isolation and culture and serological detection. The former was once the "gold standard" for the diagnosis of herpes virus, but it takes a long time (3-30 days) and has low sensitivity (especially after antiviral drug treatment). ), typing difficulties and other defects; the latter is divided into direct antigen detection method and antibody indirect detection method. The direct antigen detection method has low sensitivity because some viruses do not produce detectable antigens, and the indirect antibody detection method is due to herpes virus It takes about 1 week after infection to produce an effective concentration of antibodies, making early diagnosis impossible, and there are cross-reactions between different herpes viruses, and the specificity of antibodies is not high

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