48results about How to "Formulated in time" patented technology

The invention discloses a method for implementing oil-gas exploration and oil-gas reservoir characterization by using living bacteria exception and total bacteria exception of methylosinus trichosporium as indexes. The method comprises the following steps of: acquiring a sample above an oil-gas exploration and oil-gas reservoir characterization area according to the specific grid degree, sampling depth and sampling quantity, and packing and storing the sample according to the specific conditions; simultaneously acquiring the living bacteria number and the total bacteria number of the methylosinus trichosporium in the sample by adopting a conventional microbe counting method, and drawing an equal living bacteria line and an equal total bacteria line on a map, a topographic map, a geologic map or a topographic-geologic map to obtain the living bacteria exception and the total bacteria exception; comprehensively comparing the living bacteria exception with the total bacteria exception; making a judgment and a prediction or providing an oil-gas reservoir exploitation scheme for the underground oil-gas resource condition of the oil-gas exploration and oil-gas reservoir characterization area according to the characteristics, similarities and differences of the living bacteria exception and the total bacteria exception by combining the results of geologic exploration, geochemical exploration and geophysical exploration.

The invention provides a quadruple fluorescent quantitative polymerase chain reaction (PCR) detection kit for diarrheagenic Escherichia coli. The quadruple fluorescent quantitative PCR detection kit consists of fluorescent quantitative PCR reaction liquid, a standard substance O157, a standard substance H7, a standard substance O104, a standard substance H4 and a reference substance, wherein the fluorescent quantitative PCR reaction liquid comprises PCR reaction buffer solution (which contains a mixture of magnesium chloride and deoxyribose nucleotide triphosphate, heat-resisting thermus aquaticus deoxyribonucleic acid (TaqDNA) polymerase and the like), special upstream and downstream primers and a probe. In the quadruple fluorescent quantitative PCR detection kit, O157:H7 and O 104:H4 type bacteria of the diarrheagenic Escherichia coli can be detected from a sample; and compared with the conventional ordinary PCR method and single fluorescent quantitative PCR method, the quadruple fluorescent quantitative PCR detection kit has the advantages of simplicity, convenience, quickness and accuracy; and the detected bacteria are quantitated accurately in real time, and the diagnosis of suspected patients infected with the bacteria clinically is clarified early, so that the treatment scheme is formulated in time to lower the death rate.

The invention discloses a downhole rescue system which is connected with a monitoring center. The downhoel rescue system comprises a breathing mask, an air source conversion system, a first air supply system and a second air supply system, wherein the air source conversion system can be switched in the first air supply system or the second air supply system to supply air to the breathing mask. The downhole rescue system enables a user to automatically switch to a standby air source under the emergency that a motor air supply system breaks down, precious time is prolonged, secondary disasters are prevented from occurring in the rescue process to cause unnecessary property loss and casualties, and therefore downhoel rescue operation can be carried out timely, effectively and interactively.