Preparation method of high-intensity anti-ultraviolet silk fibroin film

Abstract

The invention discloses a preparation method of high-intensity anti-ultraviolet silk fibroin film. The preparation method comprises the following steps: dissolving degummed mulberry silk in an imidazole carboxylate ion liquor to prepare a 10-15wt% silk fibroin solution, performing filter pressing and static defoaming, then naturally spreading the silk fibroin solution on a glass plate by adopting a tape casting method, soaking the glass plate in an absolute ethyl alcohol solution for condensation, washing with water at normal temperature, and naturally drying to obtain the silk fibroin film. The breaking strength of the silk fibroin film is larger than 15Mpa, the elongation at beak is larger than 20%, and the breaking strength of the film can be over 12Mpa after the film is irradiated under an ultraviolet lamp. The film is nontoxic and harmless, has a good biocompatibility and a characteristic of high intensity, and can be used as surgical restorative auxiliary materials such as an artificial skin material and a drug slow-release carrier. A preparation process is simple, the solution can be recycled, and the silk fibroin film is environment-friendly and has good application prospect.

Description

technical field [0001] The invention belongs to the fields of material science, biochemistry and protein chemistry, and in particular relates to a preparation method of a high-strength silk fibroin film. Specifically, degummed mulberry silk is used as raw material and imidazole carboxylate ionic liquid is used as solvent to prepare a suitable film-forming stock solution; after coagulation, washing, drying and other processes, a high-strength anti-ultraviolet silk fibroin film is prepared. Background technique [0002] Silk fibroin is the main component of natural silk, which accounts for 70-80% of the silk, and is obtained by degumming silk. Silk fibroin has good biocompatibility and degradability with the human body, and has a wide range of applications in the biological field. Among them, silk fibroin film is the earliest and most deeply studied silk fibroin material. It is obtained by drying silk fibroin solution. Its biggest disadvantage is its high brittleness and poor...

AI Technical Summary

Problems solved by technology

Although the above methods can improve the mechanical properties of silk fibroin films to varying degrees, the introduction of foreign species will result in uneven degradation of the obtained silk fibroin films and a decrease in biocompatibility, which affects its application range. Harsh process conditions, easy to produce harmful chemical residues and environmental pollution

It seems that the existing production process is difficult to produce silk fibroin film with good mechanical properties

The main reason for the high brittleness and poor mechanical properties of the silk fibroin film is that the C-N bond length of the peptide bond -CO-NH- on the silk fibroin protein macromolecular peptide chain is 0.132nm, which is 0.147nm longer than the C-N single bond length. The nm is a little shorter, longer than the 0.127nm bond length of the C=N double bond, so that the peptide chain has the property of part of the double bond, and the rigidity is relatively high, which affects the flexibility of the main chain of the silk fibroin protein macromolecule

In the process of film formation, the structure of silk fibroin will change from random curl to β structure, so that a large number of hydrogen bonds can be formed between side chains, between side chains and main chains, and between molecules. A large number of secondary cross-linking points are generated, making it more difficult for the macromolecules of silk fibroin to move, resulting in poor softness, elongation and elasticity of the silk fibroin film