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Application of betaine hydrochloride in preparing medicines and health foods for preventing and treating hepatic diseases

A technology for betaine hydrochloride and liver disease, which is applied in the application field of medicine and achieves the effects of simple chemical synthesis, low price, and enhanced virus effect

Inactive Publication Date: 2014-05-21
张晓元
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high frequency of non-response (immune tolerance) to IFN in the treatment of CHB and CHC has become a severe clinical challenge

Method used

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  • Application of betaine hydrochloride in preparing medicines and health foods for preventing and treating hepatic diseases
  • Application of betaine hydrochloride in preparing medicines and health foods for preventing and treating hepatic diseases
  • Application of betaine hydrochloride in preparing medicines and health foods for preventing and treating hepatic diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Anti-hepatitis B virus effect of betaine hydrochloride and its combination with α-interferon in vitro

[0030] HepG 2.2.1.5 cell line is a HBV-DNA transgenic liver cancer cell line established abroad that can actively secrete hepatitis B virus. It is derived from HepG2 liver cancer cells. Interferon can inhibit the proliferation of hepatitis B virus through its intracellular signaling pathway, and lamivudine is a nucleoside analog that can directly inhibit viral DNA replication. By detecting the contents of HBsAg, HBeAg and HBV DNA in the culture supernatant of HepG 2.2.1.5 cells, the antiviral effect of lamivudine and the biological activity of α-interferon can be identified. In this example, betaine hydrochloride was used in combination with α-interferon, and lamivudine was used as a control to act on the 2.2.1.5 cell line to observe their inhibitory effect on the proliferation of hepatitis B virus.

[0031] 1. Materials and methods

[0032] 1.1 Materials...

Embodiment 2

[0065] Embodiment two, the inhibitory effect of betaine hydrochloride on the resistance mutation of the nucleoside antiviral drugs of HBV-DNA

[0066]Principle: Nucleoside anti-HBV drugs can induce drug-resistant mutations in HBV to varying degrees, thus increasing the difficulty of clinical treatment. HepG2.2.15 cells can secrete HBV virus particles, and lamivudine can increase the proportion of HBV mutants in the cells. This example designs a method for detecting low-abundance HBV mutants, and observes the inhibitory effect of betaine hydrochloride on HBV mutations induced by lamivudine.

[0067] 1 Materials and methods:

[0068] Cell culture and general materials are the same as in Example 1.

[0069] 1.1 Cell culture: with embodiment 1.

[0070] 1.2 Construction of plasmids containing YMDD, YVDD, and YIDD respectively: HBV-DNA was used as a template and s-YMDD in Table 6 was used as a primer to amplify a 600bp HBV-DNA fragment centered on YMDD. After digestion with Hin...

Embodiment 3

[0093] Embodiment three, the antiviral action of betaine hydrochloride to duck hepatitis B virus

[0094] Principle: Duck hepatitis B virus (DHBV) is a hepatitis model of duck natural infection, and is a commonly used animal drug efficacy model for testing drugs against the proliferation of hepatitis B virus. It is known that lamivudine can directly inhibit the DNA replication of hepatitis B virus in ducks. By detecting the content of DHBV-DNA in duck serum, the antiviral activity of the drug can be determined. In this example, lamivudine was used as a control to observe the anti-hepatitis B virus effect of betaine hydrochloride.

[0095] 1. Materials and methods

[0096] 1.1 Reagents: 32P-dCTP and Notch Translation Kit (Promega); Betaine Hydrochloride (Amresco); Lamivudine (LAM, GlaxoSmithKline).

[0097] 1.2 Animals: Male one-day-old Longyan Shelduck, purchased from the hatchery of Chaoyang Village, Shijing, weighing about 50 grams per bird, was reared conventionally. Br...

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Abstract

The invention discloses application of betaine hydrochloride in preparing medicines and health foods for preventing and treating hepatic diseases. Betaine hydrochloride is particularly used for preparing medicines and health foods for treating virus B hepatitis and hepatitis C, preparing sensitizers or synergists in viral disease treatment by interferon, preparing medicines or functional foods for reducing drug tolerance and the like occurring when hepatitis B is treated by nucleotide medicines, and preparing medicines or functional foods for protecting hepatic cells of hepatic diseases and reducing transaminase.

Description

technical field [0001] The invention relates to a new application of betaine hydrochloride, in particular to the application of the compound as a medicine for treating liver disease viral diseases. Background technique [0002] my country is a high-incidence area of ​​viral hepatitis. About 90 million people have been carrying hepatitis B virus for a long time, of which 1 / 4 eventually develop into chronic hepatitis B. Hepatitis C is also a common viral hepatitis, which is widely prevalent in the world and is the most important cause of liver cirrhosis and liver cancer in Europe, the United States and other countries; acute infection of hepatitis C is more likely to develop into chronic infection than hepatitis B , and cirrhosis of the liver can also occur. There are 170 million hepatitis C patients in the world, and more than 30 million hepatitis C infections in my country. Every year in my country, 340,000 people die from hepatitis B infection, 330,000 people die from hep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/205A61P31/20A61P31/14A61P1/16A23L1/305A23L33/10
Inventor 张晓元银巍李奎王自峰
Owner 张晓元
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