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Efficient phosphorus dissolving aspergillus niger and application thereof

A technology of Aspergillus niger and microbial inoculants, applied in the field of microorganisms and biological fertilizers, can solve problems such as soil water retention decline, grassland degradation, soil compaction, etc.

Inactive Publication Date: 2014-05-21
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, 74% of the cultivated land in my country is deficient in phosphorus, and more than 95% of the phosphorus in the soil is in an invalid form, which is difficult for plants to directly absorb and utilize. In addition, the extensive use of chemical fertilizers has obviously caused soil compaction, soil water retention, grassland degradation, and serious desertification. Adverse consequences

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Microorganisms from the isolation of soil samples

[0016] From the chrysanthemum rhizosphere soil collected near Hengshui Lake, Hebei, 10g of soil was weighed and mixed with 90mL sterile water, and then 1mL samples were taken from it and diluted with 9mL sterile water, which was carried out 6 times and diluted to 10 -6 、10 -7 、10 -8 3 gradients, pipette 100 microliters of the bacterial suspension of the last three gradients, and spread on Montina inorganic phosphorus medium (glucose 10 g, (NH 4 ) 2 SO4 0.5 g, NaCl 0.3 g, KCl 0.3 g, FeSO47H2O 0.03 g, MnSO44H2O 0.03 g, MgSO47H2O 0.3 g, phosphate rock powder 10 g, yeast extract 0.4 g, agar 15 g, distilled water 1000 ml, pH 7.0~7.5) on the plate , with the last dilution as a control, cultured at 28°C, and observed every 24h. Select a strain that has no colonies in the control but grows colonies in the previous dilution, jumps a single colony according to the characteristics of the colony shape, color, etc....

Embodiment 2

[0017] Example 2 Phosphorus Screening of strains

[0018] In a sterile environment, all strains were inoculated on Montina medium and cultured at 30°C for 7 days. Observe the growth of the colonies in the plate, and screen out highly efficient phosphorus-solubilizing bacteria according to the size of the phosphorus-dissolving circle.

Embodiment 3

[0019] Example 3 Determination and Analysis of ITS Sequence and Identification of Physiological and Biochemical Tests of Bacterial Strains

[0020] ITS identification: the spore liquid of the screened strain was put into PD liquid medium, cultured on a shaker at 28°C for 48 hours, the hyphae were obtained by filtration, and the fungal genomic DNA was extracted. Using the extracted fungal DNA as a template, PCR amplification was performed with ITS5 and ITS8 primers. The PCR reaction system is 25 μl, and the reaction system is: 1 μL of genomic DNA, 2.5 μL of 10×PCR buffer, 1 μL of NS1 primer, 1 μL of NS8 primer, 2 μL of dNTPs, 0.25 μL of Taq enzyme, and 17.25 μL of ddH2O. The reaction conditions were: 94°C for 5 min, 94°C for 30 s, 57°C for 50 s, 72°C for 50 s; cycle 30 times; 72°C for 10 min. After the PCR amplification product was sent to Shanghai Sangon Biotechnology Co., Ltd. for sequencing, the obtained DNA sequence was input into NCBI, and the Blast program was used to ...

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PUM

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Abstract

The invention relates to efficient phosphorus dissolving aspergillus niger. The bacillus subtilis is collected with the collection number of CGMCC (China General Microbiological Culture Collection Center) No.8240 in the CGMCC. The aspergillus niger provided by the invention is relatively high in phosphorus dissolving effect, free of environment pollution and ecologically safe. A compound biofertilizer prepared by singly using the efficient aspergillus niger or using the efficient aspergillus niger and organic or inorganic nutritional ingredients required by the growth of other plants has the effects of increasing the yield and improving the quality.

Description

technical field [0001] The invention relates to the field of microorganisms and biological fertilizers, in particular to Aspergillus niger, in particular, the invention relates to an Aspergillus niger with high-efficiency phosphorus-solubilizing effect and an application thereof. Background technique [0002] Phosphorus fertilizer can promote plant flower bud differentiation, early flowering and fruiting, promote seedling root growth and improve fruit quality. Phosphorus application can promote the normal progress of various metabolisms, good plant growth and development, and at the same time improve the cold resistance and drought resistance of plants. Since phosphorus is related to the metabolism of carbohydrates, proteins and fats and the mutual transformation of the three, phosphorus fertilizers are often required regardless of the cultivation of food crops, bean crops and oil crops. When phosphorus is deficient, protein synthesis is blocked, new cytoplasm and nucleus a...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/685
Inventor 时翠平赵斌霍静倩董金皋张金林齐萌
Owner HEBEI AGRICULTURAL UNIV.
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