Method for cultivating seaweed and method for producing alginic acid-containing composition

A culturing method and composition technology are applied in the field of algae cultivation and the preparation of compositions containing alginic acid, and can solve the problems of expensive equipment, a large amount of electric energy, and microbial contamination of equipment culture solution.

Inactive Publication Date: 2014-05-28
KANEKA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, depending on the weather, seasons, etc., the amount of light exposure changes, and there is also a problem that the culture medium is easily contaminated by microorganisms and the like in terms of equipment.
In order to solve the above problems, although there is also a method of irradiating and cultivating with artificial light such as electric lighting in a completely closed system, this not only requires expensive equipment, but also requires a large amount of electric energy.

Method used

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Examples

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Effect test

preparation example Construction

[0068] In addition, the present invention also includes a method for producing an alginic acid-containing composition, characterized by a step of producing an alginic acid-containing composition from the culture obtained by the above-mentioned cultivation method of the present invention. In other words, heterotrophic culture of algae belonging to the genus Parachlorella (Parachlorella) capable of producing alginic acid and use of the obtained culture to prepare an alginic acid-containing composition belong to the scope of the present invention.

[0069]The composition containing alginic acid in the present invention is not particularly limited as long as it is a composition containing alginic acid and / or a salt thereof, and may contain algae-derived components, algae bodies, or its salts in addition to alginic acid. Compositions such as crushed products may contain high-purity alginic acid itself after removal of algal bodies and the like. That is, as the alginic acid-containi...

Embodiment 1

[0089] Glucose 20g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate heptahydrate 1g / L, calcium chloride 0.01g / L, urea 2g / L, ferric sulfate heptahydrate 5mg / L, Arnon's A5 solution 1mL / L ( Composition of Arnon's A5 solution: boric acid 2.86g / L, manganese chloride tetrahydrate 1.81g / L, copper sulfate tetrahydrate 0.08g / L, zinc sulfate heptahydrate 0.22g / L, ammonium molybdate tetrahydrate 0.171g / L) 100 mL of the formed culture medium (pH 6.5) was sterilized in a shaker flask, inoculated with Parachlorella sp.binos FERM BP-10969, protected from light with aluminum foil, and cultured with shaking at 30°C for 72 hours. After the cultivation was completed, the content of the dried algae body in the culture solution was determined to be 9.0 g / L. In addition, when the concentration of alginic acid in the culture solution was measured, it was 0.52 g / L in terms of sodium alginate. That is, the yield of alginic acid per 1 kg of the dried algal bodies was 58 g.

Embodiment 2

[0091] Put 100 mL of the culture medium with the same composition as in Example 1 into a shaker flask for sterilization, inoculate Parachlorella sp.binos FERM BP-10969, protect from light with aluminum foil, shake in the dark at 30°C for 72 hours, and perform pre-culture. Next, glucose 10g / L, potassium dihydrogen phosphate 1.5g / L, magnesium sulfate heptahydrate 1.5g / L, calcium chloride 20mg / L, urea 3.5g / L, ferric sulfate heptahydrate 10mg / L, citric acid 0.1 g / L, Arnon's A5 solution (the composition of Arnon's A5 solution is the same as in Example 1) 1mL / L, Adekanol LG109 (manufactured by ADEKA Corporation) 0.1g / L The culture medium 2000mL that forms is put into the fermentation tank of 5L capacity after sterilization , inoculated with 200 mL of the above-mentioned pre-culture solution, protected from light with aluminum foil, and cultured for 143 hours at an internal temperature of 30°C, an aeration rate of 2 L / min, a stirring rate of 450 rpm, and a pH of 6-7. A 30% (w / w) aque...

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Abstract

The problem of the present invention is to provide a method for industrially and efficiently cultivating seaweed having alginic acid-producing capability and an industrially advantageous method for producing an alginic acid-containing composition. The method for cultivating seaweed relating to the present invention is characterized in comprising a step for heterotrophically cultivating seaweed having alginic acid-producing capability belonging to the genus Parachlorella. Moreover, the method for producing an alginic cid-containing composition relating to the present invention comprises a step for obtaining an alginic acid-containing composition from a seaweed cultivation product obtained by the cultivation method.

Description

technical field [0001] The present invention relates to a method for cultivating algae belonging to the genus Parachlorella (Parachlorella) and capable of producing alginic acid. In addition, the present invention relates to an industrially advantageous method for preparing a composition containing alginic acid. Further, the present invention relates to: dried algal bodies obtained from the alginic acid-containing composition obtained according to the preparation method, a sludge reducing agent containing the alginic acid-containing composition, a harmful substance adsorbent, feed, food and pharmaceuticals. Background technique [0002] Alginic acid is a viscous polysaccharide contained in the cells and cell walls of brown algae or some red algae. It is two kinds of uronic acids, namely D-mannuronic acid and L-guluronic acid. Polyuronides polysaccharides combined in various ratios. Alginic acid or its salt, as a thickening stabilizer and gelling agent, is not only widely ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12A23K1/16A23L1/30A61K31/734A61P1/12A61P3/06A61P3/10A61P9/10A61P9/12B01J20/24B01J20/30C12P7/44C12R1/89
CPCA61K31/734A23L1/30C12N1/12A23K1/1643A23K1/14C12P7/44A61K36/05A23K1/16C12P19/04A23L1/3002B01J20/30A23L1/308A23K1/006C02F1/68B01J20/24C12R1/89A23K10/30A23K20/163A23K10/10A23L33/10A23L33/105A23L33/21A61P1/12A61P3/06A61P3/10A61P9/10A61P9/12B01J20/3078B01J2220/4843C12N1/125C12R2001/89Y02W10/37
Inventor 木崎宪之古田武毛利拓田冈直明
Owner KANEKA CORP
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