Glucosamine produced by enzymic method and preparation method of glucosamine

A technology of glucosamine and enzymatic method, which is applied to the field of glucosamine produced by enzymatic method and its preparation, can solve the problems of severe hydrolysis conditions, harmful hydrochloric acid, long production cycle and the like, and achieves rapid hydrolysis, short production cycle and reduced production cost. Effect

Inactive Publication Date: 2014-06-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many disadvantages of this method, for example: 1) The product is glucosamine hydrochloride, not glucosamine monomer; 2) The production cycle is long; 3) High concentration of hydrochloric acid will destroy the product to a certain extent; 4) Hydrolysis conditions It is more violent and consumes a lot of energy; 5) Residual hydrochloric acid is not only harmful to the human body, but also harmful to equipment and the environment
Although some people are committed to changing this production method, such as adding 0.5% to 2% oxalic acid when adding hydrochloric acid, it has not substantially changed this production method, and the shortcomings are still outstanding.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: It is the basic embodiment of the glucosamine produced by enzymatic method of the present invention. The glucosamine produced by the enzymatic method uses chitosan as a substrate, adds water and glacial acetic acid to form a colloid, then adds endochitosan and exochitosan for enzymolysis, and removes the glucosamine after enzymolysis is completed. protein, and then dry the enzymatic hydrolysis solution to obtain a regular form of glucosamine, which is a monosaccharide of amino; the regular form of the product is powder, and can also be processed into granules or flakes.

Embodiment 2

[0050] Example 2:It is the basic embodiment of the preparation method of glucosamine produced by enzymatic method of the present invention. The method steps include:

[0051] A. Put chitosan into the reactor, add water while stirring, and set the volume to a certain concentration;

[0052] B. After swelling, add glacial acetic acid while stirring to form a uniform colloid with a certain pH value;

[0053] C. After forming a uniform colloid, heat the reactor to a suitable temperature;

[0054] D, add chitosan endonuclease and chitosan exonuclease in reactor;

[0055] E. Maintain a suitable temperature for a certain period of time until the enzymatic hydrolysis is sufficient;

[0056] F, after the enzymatic hydrolysis is sufficient, remove the enzyme, and obtain the enzymatic hydrolyzate;

[0057] G. Drying the enzymolysis solution to obtain glucosamine.

Embodiment 3

[0058] Example 3: It is a preferred embodiment of the enzymatically produced glucosamine preparation method of the present invention. The glucosamine preparation method of described enzymatic production, its steps are: A, get chitosan and be placed in reactor, add water while stirring with the stirring speed of 100~800rpm, make chitosan swell, and make chitosan The sugar concentration is 0.5% to 30%; B. After the swelling is sufficient, add glacial acetic acid while stirring at a stirring speed of 100 to 800rpm. The ratio V / W of glacial acetic acid to chitosan is 0.1 to 1, and stir until the chitosan Form a uniform colloid, and finally make PH4 ~3×10 4 ; The ratio U / kg of chitosan exonuclease and chitosan is 1×10 4 ~5×10 4 The addition ratio of chitosan endosugar and chitosan exonuclease is 0.06~5; E, keep temperature 30~60 ℃ after adding enzyme, make reaction fully through 2~5 hours, make its monosaccharide yield in Between 70% and 90%; F. After the reaction is completed...

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PUM

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Abstract

The invention relates to glucosamine produced by using an enzymic method and a preparation method of the glucosamine. According to the glucosamine produced by using the enzymic method, the glucosamine, namely amino monosaccharide is obtained by taking chitosan as a substrate, adding water and glacial acetic acid to form colloid, then adding chitosan endonuclease and chitosan exonuclease for enzymolysis, removing protein after enzymolysis, and drying the enzymatic hydrolysate. The preparation method comprises the following steps: A, adding water to the chitosan to meter the volume; B, adding glacial acetic acid to uniformly mix; C, heating; D, adding the chitosan endonuclease and the chitosan exonuclease; E, carrying out heat preservation for enzymolysis; F, removing the enzyme to obtain the enzymatic hydrolysate; G, drying the enzymatic hydrolysate. The glucosamine and the preparation method disclosed by the invention have the advantages that the glucosamine is the amino monosaccharide, which is beneficial to the human body to absorb without side effects; the bio-enzyme is used as a catalyst, so that the production cost is greatly reduced; the production procedures are simple, and the product quality is stable; the production period is short, and the energy consumption is low; the enzyme hydrolysate does not contain hazardous substances, thus the glucosamine product is green and environment-friendly and free of environmental pollution.

Description

technical field [0001] The invention relates to an enzymatically produced glucosamine and a preparation method thereof, belonging to the technical field of monosaccharide production. Background technique [0002] Glucosamine is a natural amino monosaccharide and the precursor of glycosaminoglycan (mucopolysaccharide), which is the main component of articular cartilage, so glucosamine is often used as an adjuvant treatment for osteoarthritis. Supplementing with glucosamine may help rebuild cartilage and treat arthritis. [0003] At present, glucosamine is mainly produced by hydrolyzing chitosan with concentrated hydrochloric acid at high temperature. This method uses chitosan as a raw material, uses 2 to 3 times the amount of hydrochloric acid as a hydrolyzing agent, and hydrolyzes at a high temperature of 85 to 95 ° C for 3 to 5 hours. There are many disadvantages of this method, for example: 1) The product is glucosamine hydrochloride, not glucosamine monomer; 2) The produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26
Inventor 彭楠梁运祥王林春
Owner HUAZHONG AGRI UNIV
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