A kind of RNA preservation liquid, the test kit comprising this preservation liquid and application thereof

Abstract

The invention discloses a RNA preserving fluid, a kit containing the preserving fluid and an application of the preserving fluid, belonging to the field of molecular biology. The preserving fluid is a 3.8M to 5.2M betaine solution; the preserving fluid can be prepared into a commercial kit and the like according to demands. By adopting the preserving fluid, a RNA sample can be preserved at a low temperature in a liquid state, and can be completely preserved for at least one year at minus 20 DEG C, or can be completely preserved for one week at 4 DEG C, and physical damage to the RNA sample due to repeated freezing and melting can be avoided. Moreover, not only is the enzyme reaction efficiency in reaction systems of inverse transcription, PCR and the like not affected, but also the reaction efficiency and the treatment on long chain RNA are positively affected, and the preserving fluid can be used for dissolving RNA which is in excessive vacuum dry preservation. The preserving fluid can effectively avoid influence of physical damage, RNase degradation, hydrolysis, container adsorption loss, bacterial pollution and the like when RNA is dissolved, preserved and used, and particularly has great application values in preservation of micro RNA, repeated use and transportation.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to an RNA preservation solution, a kit containing the preservation solution and applications thereof. Background technique [0002] Ribonucleic acid (RNA, Nuecleic Acid) is one of the main components of cells and participates in various functional activities of cells. It is an important object of research in biology, medicine, pharmacy and other life-related disciplines. [0003] In most studies involving RNA expression or diagnostic and prognostic assays for medical applications, highly purified RNA is required as a substrate for the reaction. However, purified RNA is easily degraded by ribonucleases (i.e., RNases) and fragmented by freeze-thaw processes. RNase, which is widespread and extremely stable in biological samples, has a strong hydrolysis effect on RNA, and many RNases do not require any cofactors to degrade RNA, which brings great difficulties to the purification, stora...

AI Technical Summary

Problems solved by technology

However, although formamide can inhibit the RNase reaction, it can also cause RNA denaturation and increase the difficulty of dissolution. Direct use of formamide-dissolved RNA for enzyme reactions will have an uncertain impact on the reaction efficiency. Re-precipitation is required before use. Removal of formamide greatly limits its application

[0006] In addition, RNA can be stored at -20°C after ethanol precipitation for long-term storage, but each time the RNA needs to be centrifuged, washed and dissolved again, this will introduce additional errors to the RNA verification, affect the repeatability of the measurement, and do not help the RNA repeated use

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