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Method for detecting content of phosphatidylserine in food

The technology of a phosphatidylserine and a detection method is applied in the detection field of phosphatidylserine content in food, and can solve the problems of difficult separation of ps, affecting ps detection, and difficulty in detection, and achieves high sensitivity, high accuracy and low detection limit. Effect

Active Publication Date: 2014-06-25
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When ps is taken as a health food, due to its high purity and high content, it can be detected by general high performance liquid chromatography without complicated pre-treatment technology. When ps is added to food as a common food raw material, its added The amount is generally small, and it is difficult to detect the interference of other raw materials in food, especially in foods mainly composed of protein, fat, starch and other substances, because protein, fat, starch and other substances will have a certain embedding effect on ps , so that ps is not easy to separate from these substances, thus affecting the detection of ps

Method used

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  • Method for detecting content of phosphatidylserine in food
  • Method for detecting content of phosphatidylserine in food
  • Method for detecting content of phosphatidylserine in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Prepare the ps standard to a concentration of 50 mg / 100mL, that is, 0.5 μg / μL, and inject 10 μL (ps content 5 μg), 30 μL (ps content 15 μg), 50 μL (ps content 25 μg), 80 μL (ps content 40μg), with ps content as the abscissa and peak area as the ordinate, the linear regression equation obtained is the standard curve, and the regression equation is y=4132.3x-2956.2, R2=0.9919.

[0026] HPLC analysis conditions:

[0027] Phosphatidylserine (ps) standard sample: sigma company, product name: L-A-PHOSPHATIDYL-SERINE FROM SOYBEAN

[0028] Instrument: Shimatzu HPLC10VP series

[0029] Column model: LiChrosphere100diol (Merck): 4mm (inner diameter) × 125mm (column length) (Merck), particle size: 5μm

[0030] Pre-column: LiChrosphere diol pre cartridge

[0031] Column temperature: 35°C

[0032] Detector parameters: ELSD2000, ALLTECH, U.S.A

[0033] Drift tube100℃

[0034] Nebulizer gas flow rate: 2.0SLPM

[0035] Flow rate: 1.5mL / min

[0036] Gradient elution conditions: ...

Embodiment 2

[0043] Take by weighing 20g milk powder, dissolve with 40mL pure water, add the mixed solution of the chloroform and methanol of 950mL in the solution, CHCl in this mixed solution 3 : MeOH=1 (volume ratio), extract at a temperature of 15°C for 5h, filter the whole solution with a 0.2μm filter membrane, and then rotovap to dryness. Then, the evaporated matter is extracted with a mixed solution of chloroform, methanol and pure water (the preparation of the mixed solution of chloroform, methanol and pure water: the volume ratio of chloroform to methanol is 1 to prepare three Chloromethane and methanol mixed solution, then take 30mL of the mixed solution and 30mL of pure water and mix it into a mixed solution of chloroform, methanol and pure water), the whole solution is then filtered with a 0.2μm filter membrane, and the filtrate is separated by standing Layer, divided into upper and lower two-layer solution, the lower layer solution was fixed to 50mL with three filters of methan...

Embodiment 3

[0046] Weigh 20g of bread, dissolve it in 40mL of pure water, add 950mL of chloroform and methanol solution, CHCl 3: MeOH=10 (volume ratio), extract at a temperature of 70°C for 15min, filter the whole solution with a 0.2μm filter membrane, and then evaporate to dryness by rotary evaporation. Then, the evaporated matter is extracted with a mixed solution of chloroform, methanol and pure water (the preparation of the mixed solution of chloroform, methanol and pure water: the volume ratio of chloroform to methanol is 3 to prepare three A mixed solution of methyl chloride and methanol, then take 30mL of the mixed solution and mix it with 10mL of pure water to form a mixed solution of chloroform, methanol and pure water), the whole solution is filtered with a 0.2μm filter membrane, and the filtrate is allowed to stand for stratification , divided into upper and lower two-layer solution, and the lower layer solution was fixed to 50mL with three filtered methane or methanol solution...

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Abstract

The invention discloses a method for detecting the content of phosphatidylserine in food. The method comprises the following steps: (1) weighing food to be detected, preparing a sample aqueous solution, extracting with a mixed solution of trichloromethane and methyl alcohol, filtering and evaporating filtrate to dryness; (2) extracting the dried substance obtained in the step (1) by a mixed solution of trichloromethane, methyl alcohol and water, filtering and standing filtrate; and (3) carrying out high performance liquid chromatography detection on a solution at an under layer. The method has the advantages of high sensitivity, high precision and high accuracy.

Description

technical field [0001] The invention relates to the field of food detection. More specifically, it relates to a method for detecting the content of phosphatidylserine in food. Background technique [0002] Phosphatidylserine (abbreviated as ps) is a phospholipid substance that plays an important role in the health of the human brain and nervous system. At present, PS can be added to food as a food raw material or taken as a health food. When ps is taken as a health food, due to its high purity and high content, it can be detected by general high-performance liquid chromatography without complicated pretreatment technology. When ps is added to food as a common food raw material, its added The amount is generally small, and it is difficult to detect the interference of other raw materials in food, especially in foods mainly composed of protein, fat, starch and other substances, because protein, fat, starch and other substances will have a certain embedding effect on ps , so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/14
Inventor 石丹王晓彦刘彪
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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