A kind of preparation method of liquid compound enzyme with high stability for feeding

Abstract

The invention relates to a process for preparing a high-stability feed liquid compound enzyme. The process comprises the steps of producing a fermentation liquid by using a deep industrial liquid fermentation method, and then refining and purifying single enzymes respectively, wherein the prepared uniform, stable, clear and transparent single enzyme liquid can be preserved for long time, and the single enzymes are compounded in any ratio according to needs to obtain a stable finished enzyme liquid. By adopting the method of preparing the stable single enzyme liquid and then compounding the single enzyme liquid in any ratio according to the needs, the process is advanced, the equipment is simple, the operation is flexible, the single enzyme liquid before compounding is high in purity and good in stability and can be stored for long time, the compounded finished enzyme is extremely high in stability, the properties of proteins in the enzyme liquid are approximate, and the problems that the equipment and material input cost in the existing process production is high and the finished enzyme is low in stability and is extremely easily precipitated and easily affected by impure enzymes so that the activity of massive enzymes is lost during storage and the enzymes cannot be flexibly compounded in any ratio are solved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of a high-purity and high-stability feed liquid compound enzyme. Background technique [0002] Enzyme is a kind of active protein, which is very sensitive to the external environment and is easily denatured and inactivated under high temperature and high pressure. At present, the addition and application of foreign enzymes in poultry and livestock feed is developing rapidly, and pellet feed accounts for a relatively large proportion of existing enzyme-added feeds. large proportion. In the production process of pellet feed, it needs to be prepared under high temperature and high pressure steam, extruded and pelletized, and then enter the cooling system. The temperature of the pellets before entering the cooling system can reach 78-90°C. The high temperature will cause the endogenous enzymes and external enzymes of the feed itself The activity of the a...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of a liquid compound enzyme with high stability for feed, which solves the problem of simple and primitive process of obtaining fermented koji material by solid fermentation method and obtaining filtrate from koji material in the preparation of existing liquid compound enzyme for feed , The finished compound enzyme has low stability and is easy to precipitate. When used, it is easy to cause the spray system to be blocked and damage the spraying equipment. It not only causes inconvenience to automatic production but also causes waste of enzyme activity; it also solves the existing process of mixing and reprocessing a variety of single enzymes In the production process, the enzyme composition ratio of the finished enzyme cannot be adjusted, and the problem of a large loss of enzyme activity during production and storage; it also solves the technical problem of high input cost of existing technology equipment and materials

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