Xylitol probiotics goat milk tablets and preparation method thereof
A technology of probiotics and xylitol, applied in milk preparations, dairy products, applications, etc., can solve the problem of less microcrystalline cellulose, and achieve the effects of promoting human health, improving sleep quality, and adjusting intestinal dysfunction
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Embodiment 1
[0030] 1) The probiotics were activated three times with MRS liquid medium at 37°C to obtain activated probiotics, and the activated probiotics were inserted into a 10L MRS medium fermenter with a 2% inoculum and cultivated for 18 hours. Stir once every 3 hours, the speed is 60 rpm, and the stirring time is 2 minutes. Then, use a centrifuge to centrifuge at a speed of 10,000 rpm for 12 minutes to make a probiotic slime, and then add probiotic bacteria per g to the probiotic slime. Add 1mL of compound freeze-drying protective agent to the mud, mix evenly with a vortex mixer, pre-freeze at -40°C for 12 hours, and then transfer to a freeze dryer for freeze-drying for 24 hours to obtain freeze-dried probiotic powder;
[0031] The compound lyoprotectant consists of 18% skimmed milk powder, 20% trehalose, 2% disodium hydrogen phosphate, 10% lactose and 50% phosphate buffer by mass percentage;
[0032] Among them, the activation process of probiotics specifically includes:
[0033] ...
Embodiment 2
[0039] 1) Take the activated probiotics and insert 3% of the inoculum into a 10L MRS medium fermenter for 18 hours. During the cultivation process, stir once every 3 hours at a speed of 60 rpm for 2 minutes. Then, centrifuge Centrifuge at a speed of 10,000 rpm for 12 minutes to make probiotic bacteria slime, then add 1mL of compound freeze-drying protective agent to the probiotic bacteria sludge per gram of probiotic slime, use a vortex mixer to mix evenly, and store at -40°C Pre-freeze for 12 hours, then transfer to a freeze dryer for 24 hours to obtain freeze-dried probiotic powder;
[0040] The compound lyoprotectant consists of 18% skimmed milk powder, 20% trehalose, 2% disodium hydrogen phosphate, 10% lactose and 50% phosphate buffer by mass percentage;
[0041] The activation process of probiotics specifically includes:
[0042] Lactobacillus acidophilus was activated three times with MRS liquid medium under the culture condition of 37°C, and Cysteine hydrochloride wi...
Embodiment 3
[0048] 1) The probiotics were activated three times with MRS liquid medium at 37°C to obtain activated probiotics, and the activated probiotics were inserted into a 10L MRS medium fermenter with a 4% inoculum for 18 hours. Stir once every 3 hours, the speed is 60 rpm, and the stirring time is 2 minutes. Then, use a centrifuge to centrifuge at a speed of 10,000 rpm for 12 minutes to make a probiotic slime, and then add probiotic bacteria per g to the probiotic slime. Add 1mL of compound freeze-drying protective agent to the mud, mix evenly with a vortex mixer, pre-freeze at -40°C for 12 hours, and then transfer to a freeze dryer for freeze-drying for 24 hours to obtain freeze-dried probiotic powder;
[0049] The compound lyoprotectant consists of 18% skimmed milk powder, 20% trehalose, 2% disodium hydrogen phosphate, 10% lactose and 50% phosphate buffer by mass percentage;
[0050] Among them, the activation process of probiotics specifically includes:
[0051] Lactobacillus a...
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