A dipstick used for testing betamethasone and application thereof
A technology of betamethasone and test strips, which is applied in the field of test strips for detecting betamethasone, can solve the problems of cumbersome operation of spectrophotometry, many influencing factors of polarimetry, unsuitable for micro-analysis, etc. The effect of short detection time and easy promotion and use
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Embodiment 1
[0040] Embodiment 1 Preparation of betamethasone detection test strip
[0041] The preparation method of this test strip mainly comprises the following steps:
[0042] 1) Prepare a conjugate release pad sprayed with betamethasone monoclonal antibody-colloidal gold marker;
[0043] 2) Prepare a reaction membrane with a detection line coated with a betamethasone hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody;
[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC bottom plate into a test strip.
[0045] The following step-by-step detailed description:
[0046] 1. Preparation of betamethasone hapten
[0047] Dissolve 0.39g of betamethasone in 10 ml of pyridine, slowly add 0.3ml of 1,3-propylenediamine dropwise at room temperature, after the dropwise addition, raise the temperature to 60°C, continue the reaction for 20h, ...
Embodiment 2
[0080] Example 2 Detection of Betamethasone Residues in Samples
[0081] 1. Sample pretreatment
[0082] Weigh 0.1g±0.01g (cream, emulsion) or 0.1ml (lotion) sample into a 10ml centrifuge tube, add 10ml 0.02mol / L phosphate buffer solution, vortex for 1min, and wait for the test.
[0083] 2. Test with test strips
[0084] Use a straw to absorb the sample solution to be tested and add 2~3 drops vertically to the sample hole, start timing when the liquid flows, react for 5~10min, and judge the result.
[0085] 3. Analyze test results
[0086] Negative (-): Both the T line and the C line are colored, indicating that the concentration of betamethasone in the sample is lower than the detection limit, such as figure 2 (a).
[0087] Positive (+): No color on the T line and color on the C line, indicating that the concentration of betamethasone in the sample is equal to or higher than the detection limit, such as figure 2 (b).
[0088] Invalid: There is no C line, indicating that...
Embodiment 3
[0089] Embodiment 3 sample detection example
[0090] 1. Detection limit test
[0091] Take a blank cosmetic sample (cream, lotion or lotion), add betamethasone to it to a final concentration of 0.5, 1, and 2mg / kg(L), take a test strip for testing, and repeat the measurement three times for each sample.
[0092] When using a test strip to test a cosmetic sample, when the concentration of betamethasone added is 0.5mg / kg (L), two red lines visible to the naked eye are displayed on the test strip, which is negative; when the concentration of betamethasone added is When it is 1.2 mg / kg (L), the quality control line of the test strip develops color, and the detection line does not develop color, which is positive, indicating that the detection limit of this test strip for betamethasone in cosmetics is 1 mg / kg (L) .
[0093] 2. False positive rate and false negative rate test
[0094] Take 20 cosmetic positive samples with known betamethasone content greater than 1 mg / kg (L) and ...
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