A dipstick used for testing betamethasone and application thereof

A technology of betamethasone and test strips, which is applied in the field of test strips for detecting betamethasone, can solve the problems of cumbersome operation of spectrophotometry, many influencing factors of polarimetry, unsuitable for micro-analysis, etc. The effect of short detection time and easy promotion and use

Active Publication Date: 2014-07-23
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The polarimetric method has many influencing factors, requires a large amount of standard reference substances and samples, and is not suitable for micro-analysis; the spectrophotometric method is cumbersome to operate, has a lot of losses during sample processing, and has a high detection limit and poor repeatability; efficient Liquid chromatography and liquid chromatography-tandem mass spectrometry are sensitive, accurate, strong specificity, and good separation, and can simultaneously determine a variety of drugs, but require expensive instruments, sample pretreatment is complex, tedious and time-consuming, and the cost of detection is relatively high. High, can not be operated on site, and requires professionals to operate, so its application is limited

Method used

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  • A dipstick used for testing betamethasone and application thereof
  • A dipstick used for testing betamethasone and application thereof
  • A dipstick used for testing betamethasone and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 Preparation of betamethasone detection test strip

[0041] The preparation method of this test strip mainly comprises the following steps:

[0042] 1) Prepare a conjugate release pad sprayed with betamethasone monoclonal antibody-colloidal gold marker;

[0043] 2) Prepare a reaction membrane with a detection line coated with a betamethasone hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody;

[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC bottom plate into a test strip.

[0045] The following step-by-step detailed description:

[0046] 1. Preparation of betamethasone hapten

[0047] Dissolve 0.39g of betamethasone in 10 ml of pyridine, slowly add 0.3ml of 1,3-propylenediamine dropwise at room temperature, after the dropwise addition, raise the temperature to 60°C, continue the reaction for 20h, ...

Embodiment 2

[0080] Example 2 Detection of Betamethasone Residues in Samples

[0081] 1. Sample pretreatment

[0082] Weigh 0.1g±0.01g (cream, emulsion) or 0.1ml (lotion) sample into a 10ml centrifuge tube, add 10ml 0.02mol / L phosphate buffer solution, vortex for 1min, and wait for the test.

[0083] 2. Test with test strips

[0084] Use a straw to absorb the sample solution to be tested and add 2~3 drops vertically to the sample hole, start timing when the liquid flows, react for 5~10min, and judge the result.

[0085] 3. Analyze test results

[0086] Negative (-): Both the T line and the C line are colored, indicating that the concentration of betamethasone in the sample is lower than the detection limit, such as figure 2 (a).

[0087] Positive (+): No color on the T line and color on the C line, indicating that the concentration of betamethasone in the sample is equal to or higher than the detection limit, such as figure 2 (b).

[0088] Invalid: There is no C line, indicating that...

Embodiment 3

[0089] Embodiment 3 sample detection example

[0090] 1. Detection limit test

[0091] Take a blank cosmetic sample (cream, lotion or lotion), add betamethasone to it to a final concentration of 0.5, 1, and 2mg / kg(L), take a test strip for testing, and repeat the measurement three times for each sample.

[0092] When using a test strip to test a cosmetic sample, when the concentration of betamethasone added is 0.5mg / kg (L), two red lines visible to the naked eye are displayed on the test strip, which is negative; when the concentration of betamethasone added is When it is 1.2 mg / kg (L), the quality control line of the test strip develops color, and the detection line does not develop color, which is positive, indicating that the detection limit of this test strip for betamethasone in cosmetics is 1 mg / kg (L) .

[0093] 2. False positive rate and false negative rate test

[0094] Take 20 cosmetic positive samples with known betamethasone content greater than 1 mg / kg (L) and ...

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Abstract

The invention discloses a dipstick used for testing betamethasone and application thereof. The dipstick consists of a sample absorption pad (1), a conjugate release pad (2), a reaction film (3), an absorbent pad (4) and a bottom patch (7), the said reaction film consists of a detection line (5) which is coated with betamethasone hapten carrier protein conjugates and a quality control line (6) which is coated with goat-anti-mouse antibody, the said conjugate release pad (2) is sprayed with a monoclonal antibody-colloidal gold marker. The invention also provides a testing method for betamethasone residue in cosmetics using the said betamethasone dipstick. The dipstick provided in this invention has characteristics of simple operation, high sensitivity, high testing speed, low cost and the like; the said dipstick is suitable for screening and field monitoring of a large number of samples.

Description

technical field [0001] The invention relates to a test strip for detecting betamethasone and an application thereof, in particular to a colloidal gold test strip for detecting betamethasone, which is particularly suitable for detecting betamethasone residues in cosmetics. Background technique [0002] Betamethasone belongs to the class of adrenal corticosteroids and has antibacterial and anti-inflammatory effects. Because of its broad-spectrum and high-efficiency bactericidal effect, it is often added to anti-acne products to achieve the purpose of anti-inflammatory, anti-acne, mite removal and anti-acne. However, this drug has many adverse reactions, such as sodium excretion and strong growth inhibitory effect; it can also cause various adverse reactions caused by corticosteroid hormones, such as musculoskeletal, gastrointestinal tract, skin, nervous system , Endocrine system abnormalities and water and electrolyte disorders. In view of the above adverse reactions, long-t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/532G01N33/558G01N33/9446
Inventor 何方洋万宇平罗晓琴吴鹏付军权蒲小容冯静彭鸽
Owner BEIJING KWINBON BIOTECH
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