A kind of 20(s)-protopanaxadiol microsphere and its preparation method and application
A technology of protopanaxadiol and panaxadiol phospholipids is applied in pharmaceutical formulations, medical preparations with inactive ingredients, medical preparations containing active ingredients, etc. The effect of improved compliance, smooth surface and simple preparation method
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Embodiment 1
[0033] Dissolve 20mg of 20(S)-protopanaxadiol and 100mg of PLGA in 1mL of a mixed solvent composed of ethyl acetate-dichloromethane (1:4 by volume) to obtain an oil phase; The obtained oil phase was slowly injected into 50mL of water phase (an aqueous solution with a mass percent concentration of 0.5% formed by polyvinyl alcohol with a degree of polymerization of 500 and an alcoholysis degree of 88%); emulsified at a high speed of 1300r / min for 5min; Continue to stir at 850r / min for 4h to completely volatilize the organic solvents (ethyl acetate and dichloromethane) in the system; centrifuge (3000r / min, 10min) to separate, wash the collected solid with distilled water 3 times, and finally ℃ vacuum drying for 36 hours, the obtained white powder is the 20(S)-protopanaxadiol microspheres described in the invention.
[0034] 1. Appearance evaluation
[0035] Suspend an appropriate amount of the obtained 20(S)-protopanaxadiol microspheres in a solution of 0.02% (w / w) Tween 80 and ...
Embodiment 2
[0068] Dissolve 20mg of 20(S)-protopanaxadiol and 160mg of PLGA in 2mL of a mixed solvent composed of ethyl acetate-dichloromethane (1:4 by volume) to obtain an oil phase; The obtained oil phase was slowly injected into 50mL of water phase (an aqueous solution with a mass percent concentration of 0.25% formed by polyvinyl alcohol with a degree of polymerization of 500 and an alcoholysis degree of 88%); emulsified at a high speed of 1000r / min for 10min; Continue stirring at 1500r / min for 4h to completely volatilize the organic solvents (ethyl acetate and dichloromethane) in the system; centrifuge (3000r / min, 10min) to separate, wash the collected solid with distilled water 3 times, and finally ℃ vacuum drying for 36 hours, the obtained white powder is the 20(S)-protopanaxadiol microspheres described in the invention. The appearance and performance evaluation results of the microspheres obtained in this example are the same as those described in Example 1.
Embodiment 3
[0070] Dissolve 20mg of 20(S)-protopanaxadiol and 80mg of PLGA in 1mL of a mixed solvent composed of ethyl acetate-dichloromethane (1:4 by volume) to obtain an oil phase; The obtained oil phase was slowly injected into 50mL of water phase (an aqueous solution with a mass percent concentration of 0.1% formed by polyvinyl alcohol with a degree of polymerization of 500 and an alcoholysis degree of 88%); emulsified at a high speed of 1300r / min for 10min; Continue to stir at 850r / min for 4h to completely volatilize the organic solvents (ethyl acetate and dichloromethane) in the system; centrifuge (3000r / min, 10min) to separate, wash the collected solid with distilled water 3 times, and finally ℃ vacuum drying for 36 hours, the obtained white powder is the 20(S)-protopanaxadiol microspheres described in the invention. The appearance and performance evaluation results of the microspheres obtained in this example are the same as those described in Example 1.
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Abstract
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