A kind of 3D uniform porous scaffold material and preparation method thereof

Abstract

The invention relates to a 3D uniform porous support material and a preparation method thereof. The existing artificial bone scaffold materials have small porosity, which limits cell migration and new tissue formation. The pore size distribution of the micropores is uneven, which is not conducive to the distribution of osteoblasts on the surface of the material. The large pore size is not conducive to cell adhesion and proliferation. The invention dissolves collagen in sodium carbonate solution, adds nano-hydroxyapatite, freezes and vacuum-dries in molds, successively undergoes cross-linking reaction in alkaline and acidic cross-linking environments, vacuum freeze-dries after washing, and sterilizes Package. The invention uses collagen and nano-hydroxyapatite as raw materials, utilizes the reaction of sodium carbonate and acid to generate carbon dioxide and the sublimation of ice crystals in vacuum freeze-drying to form a microstructure with uniform pore size distribution and suitable for cell adhesion, proliferation and migration. Pore ​​structure, the prepared uniform porous scaffold not only has a certain compressive strength but also has good microstructure and biocompatibility.

Description

technical field [0001] The invention relates to a biomedical material, in particular to a 3D uniform porous scaffold material and a preparation method thereof. Background technique [0002] The proposal and development of regenerative medicine and tissue engineering have brought new hope for the repair and treatment of bone defects clinically. With the formation of new tissue in a real sense, the tissue engineering scaffold needs to be used as a temporary support frame system at the repair site, capable of maintaining cell adhesion, migration and differentiation, and maintaining nutrients, water and metabolic excretions. The input and output, which requires the implant material scaffold to have a three-dimensional interpenetrating micro-architecture, and at the same time have a suitable pore size. Therefore, the adhesion, proliferation and climbing of cells on the surface and inside of the material are the key to the repair of new bone at the implant site. [0003] Studie...

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Problems solved by technology

When the pore size of the scaffold material is less than 100µm, the scaffold is too dense and the porosity is too small, and the small pore size will affect the migration and proliferation of cells in the material. In addition, as an implant in the body, the material with small pore size will form a hypoxic zone and The transmission of water and nutrients is limited, which is not conducive to the formation of vascularization; however, when the pore size and porosity of the scaffold material are too large, it will affect the adhesion of cells on the surface of the material and affect the communication between cells. It is not conducive to the construction of extracellular matrix

[0004] Most of the porosity of the prepared three-dimensional porous materials cannot meet the above technical requirements, and the porosity is relatively small, which limits the migration of cells and the formation of new tissues to a certain extent; or the distribution of micropores Inhomogeneous, in the process of mutual communication between the material and the body in the early stage of implantation, it is not conducive to the distribution of osteoblasts on the surface of the material, and ultimately affects the quality of new tissue; or has a relatively high pore size, which is not conducive to a certain extent The adhesion and proliferation of cells on the surface of the material affect the establishment of the extracellular matrix

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