Method for culturing bacillus subtilis through high-density fermentation

Abstract

The invention discloses a method for culturing bacillus subtilis through high-density fermentation, belonging to the field of microbial fermentation engineering. The method is used for increasing the thalli yield and spore rate of the bacillus subtilis. The method comprises the steps of activating the bacillus subtilis and performing two-step fermentation culture to produce a target product. The method has the advantages that the components of a culture medium are readily available and cheap, the process parameters are simple, the fermentation period is short, the cell biomass of the obtained bacillus subtilis can reach over 9*10<9>cfu / ml, and the spore rate is over 90%. The quality and stability of the product are significantly improved.

Description

technical field

[0001] The invention belongs to the field of microbial fermentation, and more specifically relates to a method for high-density fermentation and cultivation of Bacillus subtilis. Background technique

[0002] Bacillus subtilis ( Bacillus subtilis ) is a genus of Bacillus, Gram-positive aerobic bacteria, non-capsulated, peri-flagellated, and able to move. It is widely distributed in soil and decaying organic matter, and is easy to reproduce in withered grass juice. Subtilisin, polymyxin, nystatin, gramicidin and other active substances produced by Bacillus subtilis during the growth process, these active substances have obvious effects on pathogenic bacteria or opportunistic pathogenic bacteria of endogenous infection inhibitory effect. It can quickly consume free oxygen in the environment, cause intestinal hypoxia, promote the growth of beneficial anaerobic bacteria, and produce organic acids, reduce intestinal pH, and indirectly inhibit the growth of othe...