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Bone tissue engineering composite porous scaffold material and preparation method thereof

A technology of bone tissue engineering and porous scaffolds, applied in medical science, prostheses, etc., can solve the problems of difficult storage and high cost of bone repair substitutes, and achieve low cost, excellent osteoconduction and induction performance, and good biological activity. Effect

Inactive Publication Date: 2014-08-27
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] The present invention proposes a composite bone tissue engineering porous scaffold material and its preparation method, which is a simple preparation scheme for a three-phase composite biological material of konjac glucomannan, nano-hydroxyapatite and chitosan. The porous scaffold material It has the characteristics of low cost, simple manufacturing process, good biocompatibility, excellent bone conduction and induction performance, etc. It provides a new repair material for bone defect surgery to solve the high cost of preparation and storage difficulties of existing bone repair substitutes And other issues

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  • Bone tissue engineering composite porous scaffold material and preparation method thereof
  • Bone tissue engineering composite porous scaffold material and preparation method thereof

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Embodiment approach 1

[0032] Implementation mode one: if figure 1 and 2 As shown, the composite bone tissue engineering porous scaffold material finally prepared in this embodiment consists of konjac glucomannan, chitosan and nano-hydroxyapatite in a mass ratio of 1:1.07:4.62, with a porosity of 91.72%. The compressive strength is 0.17MPa. The specific preparation method is:

[0033] (1) Use a measuring cylinder to measure 50ml of distilled water and place it in a small beaker with a capacity of 50ml, add 4.26g of nano-hydroxyapatite and stir to fully dissolve to obtain solution A;

[0034] (2) Add 0.99g chitosan to solution A, mix and stir evenly to obtain mixed suspension B;

[0035] (3) Extract 0.097ml analytically pure ammonia water with a micro-injector, add it to the suspension B, and stir thoroughly to obtain an alkaline solution C;

[0036] (4) Add 0.92g konjac glucomannan to C at a constant speed, and take out the stirring bar quickly after 2-3 seconds after the stirring vortex disappe...

Embodiment approach 2

[0041] Embodiment 2: The composite bone tissue engineering porous scaffold material finally prepared in this embodiment consists of konjac glucomannan, chitosan and nano-hydroxyapatite in a mass ratio of 1:0.54:2.31, with a porosity of 90.28% , The compressive strength is 0.21MPa. Specific steps include:

[0042] (1) Use a graduated cylinder to measure 50ml of distilled water and place it in a small beaker with a capacity of 50ml, add 4.26g of nano-hydroxyapatite and stir to fully dissolve it to obtain solution A;

[0043] (2) Add 0.99g chitosan to solution A, mix and stir evenly to obtain mixed suspension B;

[0044] (3) Extract 0.19ml of analytically pure ammonia water with a micro-sampler, add it to the suspension B, and stir thoroughly to obtain an alkaline solution C;

[0045] (4) Add 1.84g of konjac glucomannan to C at a constant speed, and after 2~3 seconds after the stirring vortex disappears, quickly take out the stirring bar to obtain gel D;

[0046] (5) Put the sma...

Embodiment approach 3

[0050] Embodiment 3: The components of the composite bone tissue engineering porous scaffold material finally prepared in this embodiment are composed of three raw materials: konjac glucomannan, chitosan and nano-hydroxyapatite, and chitosan / konjac glucoman The polysaccharide (mass ratio) is 0.36 / 1, the nano-hydroxyapatite / konjac glucomannan (mass ratio) is 1.54 / 1, the porosity is 88.30%, and the compressive strength is 0.25MPa.

[0051] (1) Measure 50ml of distilled water with a graduated cylinder and place it in a small beaker with a capacity of 50ml, add 4.26g of nano-hydroxyapatite and stir to fully dissolve it to obtain solution A;

[0052] (2) Add 0.99g chitosan to solution A, mix and stir evenly to obtain mixed suspension B;

[0053] (3) Extract 0.29ml of analytically pure ammonia water with a micro-injector, add it to the suspension B, and stir thoroughly to obtain an alkaline solution C;

[0054] (4) Add 2.77g of konjac glucomannan to C at a constant speed, and afte...

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Abstract

The invention relates to a bone tissue engineering composite porous scaffold material and a preparation method thereof, and belongs to the application field of biomedical material technology. The porous material comprises the components: 1.4-5.0 wt% of konjac glucomannan, 5.0-8.0 wt% of nano hydroxyapatite, and 1.0-2.0 wt% of chitosan; the porosity is 70-92 wt%, and the compressive strength is 0.14 MPa-0.75 MPa. A mixed sol is prepared by mechanical mixing of the three components in an alkaline environment, and then is subjected to curing, alkali removal, freeze drying and other process treatment to obtain a konjac glucomannan / nano hydroxyapatite / chitosan composite bone tissue engineering porous scaffold. The preparation method of the scaffold has the advantages of simple preparation process, simple use equipment, short product finishing cycle, lower cost and the like; and cell and animal experiments show that the konjac glucomannan / nano hydroxyapatite / chitosan composite bone tissue engineering porous scaffold prepared by the method has quite good tissue compatibility, good cell proliferation and obvious osteogenesis effect, is expected to realize industrial expansion production, and achieves clinical application.

Description

technical field [0001] The invention relates to a composite porous support material for bone tissue engineering and a preparation method thereof, belonging to the technical application field of biomedical materials. Background technique [0002] For a long time, bone repair materials mainly adopt autologous or allogeneic bone grafts, but there are serious defects in the source of autologous bone grafts. Bone extraction from autologous heterotopia will cause new local lesions to the patient, which is tantamount to tearing down the east wall to make up for the west wall, making the patient susceptible to postoperative complications. Allogeneic bone transplantation is quite difficult and expensive in terms of material selection and storage, and is prone to immune rejection. At the same time, allogeneic bone is replaced slowly, and the volume of new bone is relatively small. In order to overcome various problems existing in autologous bone and allograft bone grafting, people tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/12A61L27/56
Inventor 陈庆华李真颜廷亭
Owner KUNMING UNIV OF SCI & TECH
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