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A kind of production method of human serum albumin

A technology of human serum albumin and its production method, which is applied in the direction of serum albumin, albumin peptide, peptide preparation method, etc., and can solve the problems of low yield of protein components, incomplete removal, and affecting product quality, etc.

Active Publication Date: 2016-10-26
ZHEJIANG HAIKANG BIOLOGICAL PROD
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Problems solved by technology

[0003] At present, the main separation method is the low-temperature ethanol method with ethanol as the precipitant. Due to the unreasonable selection of process parameters such as ethanol concentration, temperature, and pH value, the yield of protein components is low, and unstable substances in human serum albumin components (such as lipoprotein) is not completely removed; on the other hand, in the existing production process of human serum albumin, diatomite needs to be added, and diatomite inevitably contains a certain amount of aluminum, which is easy to dissolve in human body during processing. A certain amount of aluminum ions remain in the finished product of blood albumin, which will affect the quality of the product

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  • A kind of production method of human serum albumin

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Embodiment Construction

[0020] A method for producing human albumin, specifically:

[0021] (1) Merging and thawing of plasma:

[0022] Plasma with a temperature lower than -5°C is placed in a thawing tank, and warm water below 37°C is passed through the interlayer of the tank body. During the melting process, the temperature of the plasma is kept below 4°C throughout the process, and the thawed mixed plasma is obtained from the reaction;

[0023] (2) Preparation and centrifugation of component I:

[0024] Adjust the pH value of the mixed plasma to 7.00~7.40, and keep the temperature of the mixed plasma at -2.5~-3.5°C, add ethanol and adjust the volume concentration of ethanol to 8%, and adjust the ionic strength to 0.15, then centrifuge it and keep it mixed throughout the centrifugation process The plasma temperature is kept at -2.5~-3.5°C to obtain the precipitate of component I (which can be used to make fibrinogen) and the supernatant of component I;

[0025] (3) Production and pressure filtrat...

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Abstract

The invention discloses a producing method of human serum albumin. The producing method has the main technical scheme that the producing method of the human serum albumin mainly comprises the steps of merging and fusion of plasma, manufacturing and centrifugation of first ingredients, manufacturing and filter pressing of second and third ingredients, manufacturing and filter pressing of fourth ingredients, manufacturing and filter pressing of fifth ingredients, precipitate dissolution and filtering of the fifth ingredients, ultrafiltration and dialysis concentration, preparation, pasteurization inactivation, sterilization filling, incubation and inspection, wherein in the ultrafiltration and dialysis concentration step, the pH value is adjusted to 5.5 to 6.0, and the sodium chloride concentration is adjusted to 1.5 to 2.0 percent. The invention aims at providing the producing method of the human serum albumin with the advantages of low aluminum ion content and high product quality.

Description

technical field [0001] The invention relates to a protein separation and purification process in biological product technology, more specifically, it relates to a production method of human serum albumin. Background technique [0002] Human albumin is the most important functional protein in human blood. Its main physiological function is to regulate the osmotic pressure of the human body and the transport of substances in metabolism. It is mainly used clinically to treat burns, scalds, cerebral edema and liver ascites. . [0003] At present, the main separation method is the low-temperature ethanol method with ethanol as the precipitant. Due to the unreasonable selection of process parameters such as ethanol concentration, temperature, and pH value, the yield of protein components is low, and unstable substances in human serum albumin components (such as lipoprotein) is not completely removed; on the other hand, in the existing production process of human serum albumin, di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/765C07K1/34
CPCC07K14/765
Inventor 沈荣杰汤浩宇万天金庄建芬胡云刚
Owner ZHEJIANG HAIKANG BIOLOGICAL PROD
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