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A strain using high concentrations of co 2 Freshwater Chlorella exhibiting heterotrophic growth characteristics

A technology with growth characteristics and high concentration, applied in the field of microorganisms, can solve the problems of high production cost of microalgae oil and greenhouse effect, etc.

Inactive Publication Date: 2016-10-26
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a strain of freshwater chlorella that can utilize high concentrations of CO 2 Accumulate a large amount of biomass and oil, and the maximum specific growth rate, average daily carbon fixation rate, light energy conversion rate, and carbon oil conversion rate are significantly improved and are close to those in heterotrophic culture, and high-concentration CO 2 At this time, the algae strain showed some heterotrophic growth characteristics, so as to solve the problems of high production cost of microalgae oil and greenhouse effect.

Method used

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  • A strain using high concentrations of co <sub>2</sub> Freshwater Chlorella exhibiting heterotrophic growth characteristics
  • A strain using high concentrations of co <sub>2</sub> Freshwater Chlorella exhibiting heterotrophic growth characteristics
  • A strain using high concentrations of co <sub>2</sub> Freshwater Chlorella exhibiting heterotrophic growth characteristics

Examples

Experimental program
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Effect test

Embodiment 1

[0055] 1. Sample collection

[0056] In July 2010, a water sample containing green algae was collected from a freshwater lake.

[0057] 2. Efficient use of high-concentration CO 2 Separation and screening of algae strains

[0058] Separation step: use dilution coating plate method for algae separation. The collected water samples were transferred to TAP liquid medium that had been autoclaved (121°C, autoclaved for 20 min), placed in a light incubator for enrichment culture, and then diluted and spread in solid TAP medium. After a period of time, pick out a single colony on the plate to obtain pure algae species.

[0059] Screening steps: inoculate the obtained pure algae strain on a 24-well plate, place it in a closed glass box, and fill the glass box with sterile 10% CO 2 , Use 680nm to detect the growth situation, select the algae strains that grow faster, and determine the high concentration of CO 2 Under the grease content.

[0060] The parameters of microalgae culture are: temper...

Embodiment 2

[0076] In order to investigate high-concentration CO 2 Under the conditions, the energy conversion efficiency of C. sorokiniana CS-1, the medium 1 used is BG-11 medium with carbon source removed, and the formula of BG-11 medium (per liter) is as follows: 1500mg NaNO 3 , 40mg K 2 HPO 4 ·3H 2 O, 75mg MgSO 4 ·7H 2 O, 36mg CaCl 2 ·7H 2 O, 1mL trace element solution A 5 . Trace element solution A 5 The formula (per liter) is as follows: 2860mg H 3 BO 4 , 1810mg MnCl 2 ·4H 2 O, 222mg ZnSO 4 ·7H 2 O, 391mg Na 2 MoO 4 , 79mg CuSO 4 ·5H 2 O, 49.47mg Co(NO 3 ) 2 ·6H 2 O; Medium 2 is to add 16g·L to BG-11 medium with carbon source removed -1 Glucose and 6g·L -1 The soybean peptone is cultured heterotrophically, the pH is 7-8, and the medium is divided into 250 mL aerated culture flasks with a culture volume of 150 mL and sterilized at 121°C for 20 min.

[0077] Inoculate fresh C. sorokiniana CS-1 with a volume fraction of 1v / v% in the above-mentioned culture medium to control CO 2 The volume ...

Embodiment 3

[0083] To investigate CO 2 For the influence of the concentration of C. sorokiniana CS-1 polyunsaturated fatty acid, we analyzed the algae oil obtained in Example 2. The results obtained are shown in Table 1. It can be seen from the table that C. sorokiniana CS-1 can use high-concentration CO 2 Accumulates biomass and oil content while accumulating polyunsaturated fatty acid α-linolenic acid, and the content of α-linolenic acid in C.sorokinianaCS-1 will be greatly reduced when completely heterotrophic, that is to say, high concentration of CO 2 It can induce C. sorokiniana CS-1 to exhibit heterotrophic characteristics while maintaining the content of α-linolenic acid, a high-value by-product of Chlorella, which is a better culture method.

[0084] Methylation of algae oil: use CHCl to the algae oil obtained in Example 2 3 Dissolve, transfer to 1.5mL Agilent glass bottle, add 1mL 1M sulfuric acid methanol solution, fill with N 2 Seal, react at 100°C for 1 hour, cool naturally, add ...

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Abstract

The invention discloses fresh water chlorella showing heterotrophic growth characteristics with high-concentration CO2. The fresh water chlorella is classified and named as Chlorella sorokiniana, the bacterial strain number is CS-1, the culture preservation register number is CGMCC No. 9215, and the preservation date is May 5, 2014. The invention further discloses the application of the chlorella in grease production. The application can efficiently keep high-concentration CO2 constant in exhaust gas environment, the production cost of the microalgae grease is thus reduced, and superior production algae is provided for biodiesel preparation. The light energy conversion rate of the cells of the chlorella is increased and is close to the chemical energy conversion rate when the high-concentration CO2 serves as a substrate, the cells show part of heterotrophic growth characteristics, and namely, when the high-concentration CO2 is fed into the cells, the specific growth rate, the grease synthesis rate and the energy utilization efficiency of the chlorella are obviously increased and are close to those of the chlorella under the heterotrophic culture condition.

Description

Technical field [0001] The invention belongs to the field of microbial technology, relates to a kind of microalgae, and specifically relates to a strain that utilizes high-concentration CO 2 Freshwater chlorella exhibiting partial heterotrophic growth characteristics. Background technique [0002] The optical radiation density on the surface of the earth is about 985.7W / m 2 However, the light energy absorbed by higher plants through photosynthesis only accounts for 0.2-2% of the solar radiation energy. The lower photosynthetic efficiency of higher plants causes a lot of waste of solar energy. In recent years, microalgae have attracted much attention because of their high photosynthetic efficiency, fast cell growth, ability to accumulate large amounts of oil and conversion into bioenergy. Although the photosynthetic efficiency of microalgae is much higher than that of higher plants, the light energy absorbed by them only accounts for 6% of the total solar radiation energy, indicat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02P20/141
Inventor 孙芝兰陈以峰窦晓
Owner JIANGSU ACAD OF AGRI SCI
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