Method for building high-yield 5-aminolevulinic acid escherichia coli engineering strains

A technology of aminolevulinic acid and Escherichia coli, applied in the fields of metabolic engineering and microbial fermentation, can solve the problem of high cost of ALA, and achieve the effect of shortening the fermentation period and reducing the production cost
CN104004701AActive Publication Date: 2014-08-27JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2014-08-27

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Abstract

The invention discloses a method for building high-yield 5-aminolevulinic acid Escherichia coli engineering strains, and belongs to the field of metabolic engineering and micro-biological fermentation. On the basis that key enzyme glutamy tRNA reduction enzyme and glutamyl aminotransferase which are 5-aminolevulinic acid C5 synthesis paths are overexpressed through carriers pACYCDuet-1, uroporphyrinogen III synthase (UROS) hemD codes and coproporphyrinogen III oxidase (CPO) hemF codes from an escherichia coli heme biosynthetic pathway are independently expressed with pCDFDuet-1 or are expressed together, and recombination strains are constructed. By means of fermentation verification, hemD or hemF is expressed separated, hemD and hemF are expressed together, and the ALA yield is obviously improved.
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Description

technical field

[0001] The invention relates to a method for constructing a high-yield 5-aminolevulinic acid Escherichia coli engineering strain, belonging to the fields of metabolic engineering and microbial fermentation. Background technique

[0002] 5-aminolevulinic acid (5-aminolevulinic acid, ALA), the molecular formula is C 5 o 3 NH 9 , with a molecular weight of 131.13 and a melting point of 149-151°C, it is the biosynthesis of chlorophyll, heme, and vitamin B 12 and other key precursor substances. As a safe, selective, and permeable photodynamic drug, ALA has gradually attracted attention in the medical field, and has been successfully used in the diagnosis and photodynamic therapy of skin cancer, bladder cancer, digestive tract cancer, and lung cancer. In addition, as a new photoactivated pesticide with high environmental compatibility and high selectivity, ALA is widely used in the field of pesticides, such as a pollution-free green pesticide, herbicide and pla...

Claims

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