Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing teriparatide

A technology for preparing teriparatide and its preparation process, which is applied in the field of preparation of polypeptide drugs, and can solve the problems of low synthetic purity, large content of teriparatide, and high cost

Inactive Publication Date: 2014-09-03
ADLAI NORTYE BIOPHARMA CO LTD
View PDF9 Cites 29 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The present inventor prepared teriparatide by using the existing synthetic method, and found that the technical problems in the prior art are: the synthetic purity is low, the cost is high, especially the impurity Met 8 (O)-Teriparatide content is relatively large, not suitable for large-scale production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing teriparatide
  • Method for preparing teriparatide
  • Method for preparing teriparatide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Embodiment one: the synthesis of Fmoc-Phe-Wang resin

[0103] Weigh 5.00 g of Wang resin (4 mmol) with a substitution degree of 0.80 mmol / g, add it to a solid-phase reaction column, wash it once with DMF, swell the resin with DCM for 30 minutes, and drain it for later use. Weigh 7.7g Fmoc-Phe-OH (20mmol), add 2.02g HOBt (15mmol) into the beaker, dissolve with DMF 50mL, add 3.0mL DIC (20mmol) at room temperature, stir and react for 30min, add this reaction solution to the above swelling The reaction is carried out in the resin after. After reacting at room temperature for 24 hours, filter with suction and wash with DMF 6 times, then add DMF to wash 6 times, then add 1.95mL Ac 2 O (20mmol), 3.2mL DIEA (20mmol), react for 1 hour. Drained, washed 6 times with DMF, washed 3 times with DCM, washed 3 times with MeOH, washed 3 times with DCM, washed 3 times with MeOH, and obtained 6.05 g of Fmoc-Phe-Wang resin after drying. The degree of substitution after detection was 0.45...

Embodiment 2

[0104] Embodiment two: the synthesis of Fmoc-Phe-2-CTC resin

[0105] Weigh 5.00 g of 2-CTC resin (4.7 mmol) with a substitution degree of 0.940 mmol / g and add it to a solid-phase reaction column for later use. Another 1.82g Fmoc-Phe-OH (4.7mmol) was weighed, dissolved in 50mL of dried DCM, then 0.62mL DIEA (0.83mmol) was added, stirred for 5min, the solution was added to the resin for reaction, after 5min of reaction, then Add 1.24 mL DIEA (1.65 mmol) to the reaction system and continue the reaction for 40 min. Then add 4 mL of anhydrous methanol and react for 10 min. Drained, washed 3 times with DMF, 3 times with DCM, 3 times with MeOH, 3 times with DCM, 3 times with MeOH, and dried to obtain 6.0 g of Fmoc-Phe-2-CTC resin. The degree of substitution is 0.48mmol / g / .

Embodiment 3

[0106] Embodiment three: the preparation of teriparatide Wang resin

[0107] Weigh 2.22 g of Fmoc-Phe-Wang resin (1 mmol) with a substitution degree of 0.45 mmol / g, add it to a solid-phase reaction column, wash it once with DMF, and swell it with DCM for 30 minutes, using a volume ratio of 1:4 The deprotection solution composed of piperidine and DMF was reacted for 5 minutes, washed once with DMF, reacted for 10 minutes with the deprotection solution composed of piperidine and DMF with a volume ratio of 1:4, washed 6 times with DMF, and weighed 1.79 g Fmoc -Asn(Trt)-OH (3 mmol), 0.41 g HOBt (3 mmol) were added to the mixed solution of DCM and DMF with a volume ratio of 1:1, and 0.48 ml DIC (3 mmol) was added under ice-water bath for activation, then added to the above-mentioned In the reaction column with resin, react at room temperature for 2 hours, and use the ninhydrin method to detect and judge the reaction end point. If the resin is colorless and transparent, it means tha...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing teriparatide, which comprises the following steps: A) in the presence of an activator system, coupling a resin solid-phase carrier and Fmoc-Phe-OH to obtain a Fmoc-Phe-resin; B) sequentially coupling amino acids with N terminal Fmoc protection and side chain protection according to the teriparatide main chain peptide sequence by solid-phase synthesis, wherein the 16th-17th amino acids in the peptide sequence are coupled by using pseudoproline dipeptide Fmoc-Asn(Trt)-Ser(PsiMe,MePro)-OH; and C) carrying out peptide resin cracking by using a TFA (trifluoroacetic acid) cracking solution containing NH4I / Me2S, purifying the crude product by HPLC (high performance liquid chromatography), and freeze-drying to obtain the teriparatide. The content of the technical impurity Met8(O)-teriparatide is lower than 0.1%. The technique for preparing teriparatide has the advantages of high purity and low cost, is suitable for large-scale production, and can effective control the Met8(O)-teriparatide content without influencing the total yield of the teriparatide.

Description

technical field [0001] The invention relates to a preparation method of polypeptide medicine, which is a preparation method of teriparatide, a medicine used for osteoporosis treatment, which can regulate calcium and phosphorus metabolism and promote bone formation. Background technique [0002] Teriparatide, British name: Teriparatide, is a straight chain 34 peptide, the structural formula is as follows: [0003] [0004] The peptide sequence is: [0005] H-Ser-Val-Ser-Glu-Ile-Gln-Leu-Met-His-Asn-Leu-Gly-Lys-His-Leu-Asn-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu- Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Phe-OH [0006] Teriparatide Forteo (Teriparatide) is the first new bone-forming agent drug approved by the US Food and Drug Administration for the treatment of primary osteoporosis, hypogonadal osteoporosis, and postmenopausal osteoporosis . Teriparatide [hPTH(1-34)] is a synthetic polypeptide hormone, which is a 1-34 amino acid fragment of human parathyroid hormone PTH, which is an ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/635C07K1/06C07K1/04
CPCY02P20/55C07K14/635
Inventor 陈晓航路杨杨东晖周亮
Owner ADLAI NORTYE BIOPHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com