Walnut polypeptide and preparation method and application thereof
A walnut polypeptide and walnut technology are applied in the field of walnut polypeptide and its preparation, and achieve the effects of reducing production cost, easy operation and simple method.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0109] 1) The walnut cake is crushed and passed through a 40-mesh sieve to obtain walnut powder. Add deionized water equivalent to 20 times its weight to the walnut powder, stir well, and adjust its pH to 9.0 with aqueous sodium hydroxide solution, at 30-35°C Extract for 4 hours, centrifuge the extract (3000-5000r / min, 20min), collect the supernatant, adjust its pH value to 4.5 with hydrochloric acid, there is a precipitate, collect the precipitate, wash with water until neutral, and dry to obtain walnut protein powder;
[0110] 2) The obtained walnut protein powder is enzymolyzed with a special enzyme for plant protein hydrolysis (enzyme activity is 300,000 to 400,000 U / g), and the controlled enzymolysis conditions are: the substrate concentration is 20% (mass), the enzyme mass fraction is 0.1%, The enzymolysis temperature is 40°C, the pH value during enzymolysis is 9.0, and the enzymolysis time is 15 hours; after the enzymolysis is completed, the enzymolysis solution is boile...
Embodiment 2
[0112] 1) The walnut cake is crushed through a 20-mesh sieve to obtain walnut powder. Add deionized water equivalent to 30 times its weight to the walnut powder, stir well, adjust the pH to 10.0 with aqueous sodium hydroxide solution, and extract at 45°C for 2 hours , the extract was centrifuged (5000-10000r / min, 15min), the supernatant was collected, and its pH value was adjusted to 3.0 with phosphoric acid. A precipitate was precipitated, collected, washed with water until neutral, and dried to obtain walnut protein powder;
[0113] 2) The obtained walnut protein powder is enzymolyzed with a special enzyme for plant protein hydrolysis (enzyme activity is 200,000 to 300,000 U / g), and the controlled enzymolysis conditions are: the substrate concentration is 10% (mass), the enzyme mass fraction is 0.5%, The enzymolysis temperature is 55°C, the pH value during enzymolysis is 10.0, and the enzymolysis time is 10 hours; after the enzymolysis is completed, the enzymolysis solution i...
Embodiment 3
[0115] 1) The walnut cake is crushed and passed through a 60-mesh sieve to obtain walnut powder. Add deionized water equivalent to 8 times its weight to the walnut powder, stir well, adjust the pH to 12.0 with aqueous sodium hydroxide solution, and extract at 50°C for 1 hour , the extract was centrifuged (5000-8000r / min, 30min), the supernatant was collected, and its pH value was adjusted to 5.0 with phosphoric acid. A precipitate was precipitated, collected, washed with water until neutral, and dried to obtain walnut protein powder;
[0116] 2) The obtained walnut protein powder is enzymolyzed with a special enzyme for plant protein hydrolysis (enzyme activity is 500,000 to 800,000 U / g), and the controlled enzymolysis conditions are: the substrate concentration is 3% (mass), the enzyme mass fraction is 0.005%, The enzymolysis temperature is 60°C, the pH value during enzymolysis is 8.0, and the enzymolysis time is 20 hours; after the enzymolysis is completed, the enzymolysis so...
PUM

Abstract
Description
Claims
Application Information

- Generate Ideas
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com