Walnut polypeptide and preparation method and application thereof

A walnut polypeptide and walnut technology are applied in the field of walnut polypeptide and its preparation, and achieve the effects of reducing production cost, easy operation and simple method.

Inactive Publication Date: 2014-09-10

GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI

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Abstract

The invention discloses a walnut polypeptide and a preparation method and application thereof. The walnut polypeptide is prepared through enzymolysis of walnut protein powder by special enzyme for hydrolysis of plant protein, enzyme deactivation, filtration, filtrate concentration, and drying, wherein the walnut protein powder is extracted from walnut cake powder obtained by smashing walnut cake dreg, the enzymolysis conditions for the special enzyme for hydrolysis of plant protein are as follows: the concentration of a substrate is 3-30% (mass), the mass fraction of the enzyme is 0.005-10%, the enzymolysis temperature is 25-70DEG C, the pH value for enzymolysis is 7.5-12.0, and the enzymolysis time is 2-30 hours. The walnut polypeptide prepared by the method has both oxidation resistance and good antihyperlipidemic effect, and has a good application prospect in preparation of natural nontoxic antioxidant drugs or health products.

Application Domain

Hydrolysed protein ingredientsMetabolism disorder +2

Technology Topic

DrugChemistry +6

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Experimental program(5)

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[0108] Example 1

[0109] 1) Walnut cake is crushed and passed through a 40-mesh sieve to obtain walnut powder. The walnut powder is added with deionized water equivalent to 20 times its weight, stirred well, and adjusted with sodium hydroxide aqueous solution to adjust its pH=9.0 at 30~35℃ Extract for 4h, centrifuge the extract (3000~5000r/min, 20min), collect the supernatant, adjust its pH to 4.5 with hydrochloric acid, and precipitate precipitation, collect the precipitate, wash with water until it is neutral, and dry to obtain walnut protein powder;

[0110] 2) The obtained walnut protein powder is enzymatically hydrolyzed with a special enzyme for plant proteolysis (the enzyme activity is 300,000 U/g), and the enzymatic hydrolysis conditions are controlled as follows: the substrate concentration is 20% (mass), and the enzyme mass fraction is 0.1%. The enzymolysis temperature is 40°C, the pH value during enzymolysis is 9.0, and the enzymolysis time is 15h; after enzymolysis is completed, the enzymolysis solution is boiled for 20min to inactivate the enzyme, filtered, the filtrate is concentrated and dried to obtain walnut polypeptide.

Example Embodiment

[0111] Example 2

[0112] 1) Walnut cake is crushed and passed through a 20-mesh sieve to obtain walnut powder. The walnut powder is added with 30 times its weight in deionized water, stirred well, adjusted to pH=10.0 with sodium hydroxide aqueous solution, and extracted for 2 hours at 45°C , The extract is centrifuged (5000~10000r/min, 15min), the supernatant is collected, and the pH value is adjusted to 3.0 with phosphoric acid, there is precipitation, the precipitate is collected, washed with water to neutrality, and dried to obtain walnut protein powder;

[0113] 2) The obtained walnut protein powder is enzymatically hydrolyzed with a special enzyme for plant proteolysis (the enzyme activity is 200,000 U/g), and the enzymatic hydrolysis conditions are controlled as follows: the substrate concentration is 10% (mass), and the enzyme mass fraction is 0.5%. The enzymolysis temperature is 55°C, the pH value during enzymolysis is 10.0, and the enzymolysis time is 10h; after enzymolysis is completed, the enzymolysis solution is boiled for 10min to inactivate the enzyme, filtered, the filtrate is concentrated and dried to obtain walnut polypeptide.

Example Embodiment

[0114] Example 3

[0115] 1) Walnut cake is crushed and passed through a 60-mesh sieve to obtain walnut powder. The walnut powder is added with 8 times its weight in deionized water, stirred well, adjusted to pH=12.0 with sodium hydroxide aqueous solution, and extracted for 1 hour at 50°C , The extract is centrifuged (5000~8000r/min, 30min), the supernatant is collected, the pH value is adjusted to 5.0 with phosphoric acid, there is precipitation, the precipitate is collected, washed with water to neutrality, and dried to obtain walnut protein powder;

[0116] 2) The obtained walnut protein powder is enzymatically hydrolyzed with a special enzyme for plant protein hydrolysis (enzyme activity is 500,000-800,000 U/g), and the enzymatic hydrolysis conditions are controlled as follows: the substrate concentration is 3% (mass), the enzyme mass fraction is 0.005%, The enzymolysis temperature is 60°C, the pH value during enzymolysis is 8.0, and the enzymolysis time is 20h; after enzymolysis is completed, the enzymolysis solution is boiled for 30min to inactivate the enzyme, filtered, the filtrate is concentrated and dried to obtain walnut polypeptide.

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Walnut polypeptide and preparation method and application thereof

A walnut polypeptide and walnut technology are applied in the field of walnut polypeptide and its preparation, and achieve the effects of reducing production cost, easy operation and simple method.

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Description & Claims & Application Information

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