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Walnut polypeptide and preparation method and application thereof

A walnut polypeptide and walnut technology are applied in the field of walnut polypeptide and its preparation, and achieve the effects of reducing production cost, easy operation and simple method.

Inactive Publication Date: 2014-09-10
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far there is no public report on the use of walnut cake for lowering blood fat

Method used

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  • Walnut polypeptide and preparation method and application thereof
  • Walnut polypeptide and preparation method and application thereof
  • Walnut polypeptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] 1) The walnut cake is crushed and passed through a 40-mesh sieve to obtain walnut powder. Add deionized water equivalent to 20 times its weight to the walnut powder, stir well, and adjust its pH to 9.0 with aqueous sodium hydroxide solution, at 30-35°C Extract for 4 hours, centrifuge the extract (3000-5000r / min, 20min), collect the supernatant, adjust its pH value to 4.5 with hydrochloric acid, there is a precipitate, collect the precipitate, wash with water until neutral, and dry to obtain walnut protein powder;

[0110] 2) The obtained walnut protein powder is enzymolyzed with a special enzyme for plant protein hydrolysis (enzyme activity is 300,000 to 400,000 U / g), and the controlled enzymolysis conditions are: the substrate concentration is 20% (mass), the enzyme mass fraction is 0.1%, The enzymolysis temperature is 40°C, the pH value during enzymolysis is 9.0, and the enzymolysis time is 15 hours; after the enzymolysis is completed, the enzymolysis solution is boile...

Embodiment 2

[0112] 1) The walnut cake is crushed through a 20-mesh sieve to obtain walnut powder. Add deionized water equivalent to 30 times its weight to the walnut powder, stir well, adjust the pH to 10.0 with aqueous sodium hydroxide solution, and extract at 45°C for 2 hours , the extract was centrifuged (5000-10000r / min, 15min), the supernatant was collected, and its pH value was adjusted to 3.0 with phosphoric acid. A precipitate was precipitated, collected, washed with water until neutral, and dried to obtain walnut protein powder;

[0113] 2) The obtained walnut protein powder is enzymolyzed with a special enzyme for plant protein hydrolysis (enzyme activity is 200,000 to 300,000 U / g), and the controlled enzymolysis conditions are: the substrate concentration is 10% (mass), the enzyme mass fraction is 0.5%, The enzymolysis temperature is 55°C, the pH value during enzymolysis is 10.0, and the enzymolysis time is 10 hours; after the enzymolysis is completed, the enzymolysis solution i...

Embodiment 3

[0115] 1) The walnut cake is crushed and passed through a 60-mesh sieve to obtain walnut powder. Add deionized water equivalent to 8 times its weight to the walnut powder, stir well, adjust the pH to 12.0 with aqueous sodium hydroxide solution, and extract at 50°C for 1 hour , the extract was centrifuged (5000-8000r / min, 30min), the supernatant was collected, and its pH value was adjusted to 5.0 with phosphoric acid. A precipitate was precipitated, collected, washed with water until neutral, and dried to obtain walnut protein powder;

[0116] 2) The obtained walnut protein powder is enzymolyzed with a special enzyme for plant protein hydrolysis (enzyme activity is 500,000 to 800,000 U / g), and the controlled enzymolysis conditions are: the substrate concentration is 3% (mass), the enzyme mass fraction is 0.005%, The enzymolysis temperature is 60°C, the pH value during enzymolysis is 8.0, and the enzymolysis time is 20 hours; after the enzymolysis is completed, the enzymolysis so...

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Abstract

The invention discloses a walnut polypeptide and a preparation method and application thereof. The walnut polypeptide is prepared through enzymolysis of walnut protein powder by special enzyme for hydrolysis of plant protein, enzyme deactivation, filtration, filtrate concentration, and drying, wherein the walnut protein powder is extracted from walnut cake powder obtained by smashing walnut cake dreg, the enzymolysis conditions for the special enzyme for hydrolysis of plant protein are as follows: the concentration of a substrate is 3-30% (mass), the mass fraction of the enzyme is 0.005-10%, the enzymolysis temperature is 25-70DEG C, the pH value for enzymolysis is 7.5-12.0, and the enzymolysis time is 2-30 hours. The walnut polypeptide prepared by the method has both oxidation resistance and good antihyperlipidemic effect, and has a good application prospect in preparation of natural nontoxic antioxidant drugs or health products.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a walnut polypeptide and its preparation method and application. Background technique [0002] At present, there are 160 million hyperlipidemia patients in my country, including patients with hypercholesterolemia, hypertriglyceridemia and complex hyperlipidemia. The main components in blood lipids are triglycerides and cholesterol. The main harm of high triglycerides to the human body is mainly to cause atherosclerosis, blockage of blood vessels and formation of thrombus. Cholesterol is fat-soluble and cannot be directly dissolved in the blood. It must be combined with lipoproteins before it can be transported to the whole body through the blood. Cholesterol-carrying lipoproteins include high-density lipoprotein (good component) and low-density lipoprotein (bad component). When there is too much low-density lipoprotein in the blood, it deposits on the arterial wall, re...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A61P3/06A23L1/29A23L33/00
Inventor 李典鹏颜小捷杨子明黄永林卢凤来陈月圆刘金磊王磊
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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