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Method for detecting biomolecules by using porous-silicon micro-cavity biosensor and based on reflected light intensity

A technology of biomolecules and porous silicon, which is applied in the measurement of phase influence characteristics, etc., can solve the problems of expensive precision and achieve the effect of low price and simple preparation process

Active Publication Date: 2014-09-10
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0019] The above-mentioned reflection spectrum, Raman spectrum, and fluorescence spectrum detection all have the disadvantages of requiring expensive and precise corresponding spectrum analyzers

Method used

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  • Method for detecting biomolecules by using porous-silicon micro-cavity biosensor and based on reflected light intensity
  • Method for detecting biomolecules by using porous-silicon micro-cavity biosensor and based on reflected light intensity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Prepare porous silicon by electrochemical etching method: use P-type single-sided polished single-crystal silicon wafer, its resistivity is 4Ω-m, and the thickness is 100μm. Silicon wafers were ultrasonically washed with acetone, absolute ethanol, and deionized water for 15 minutes before the experiment. Perform electrochemical etching on the cleaned single crystal silicon wafer. The corrosion solution is composed of 49% hydrofluoric acid and 95% ethanol according to the volume ratio HF:CH 3 CH 2 OH=1:1 ratio mixed. The corrosion process is controlled by a current density of 200mA / cm 2 and 800mA / cm 2 The constant current source boot. First use a current density of 200mA / cm 2 , the corrosion time is 10s, the pause is 5s, and the current density is 800mA / cm 2 , the corrosion time is 10s, and then pause for 5s, so as to alternately corrode for 2 cycles. 800mA / cm for the middle microcavity 2 Pause for 10s in the middle of corrosion for 30s, and finally use a c...

Embodiment 2

[0051] The corrosion process is controlled by a current density of 300mA / cm 2 and 600mA / cm 2 The constant current source boot. Others are the same as in Embodiment 1, and the sensitivity of porous silicon is 8800±50 nm / refractive index unit.

Embodiment 3

[0053] The organic solvent in step (2) is THF, and the others are the same as in Example 1. The sensitivity of the obtained porous silicon reaches 8850±50nm / refractive index unit.

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Abstract

A method for detecting biomolecules by using a porous-silicon micro-cavity biosensor and based on reflected light intensity is characterized in that main experimental apparatuses comprise a helium-neon laser and an optical-power detector; the laser emits laser at a certain angle to a material taking porous-silicon micro-cavities as a substrate, a power receiving device receives reflected light, then an angle of a micro-cavity structure corresponding to a minimum light intensity power is found, and a biological sample in added for changing the refractive index of a porous silicon layer, and then the angle, corresponding to the minimum light-intensity power, of the reflected light is detected, and therefore, the concentration of the biological sample is detected through the variation of the angles.

Description

technical field [0001] The invention relates to a method for detecting biomolecules, in particular to a method for detecting biomolecules based on a porous silicon microcavity biosensor based on reflected light intensity. Background technique [0002] Porous silicon biosensors have the characteristics of high sensitivity, fast response, good real-time performance, label-free, remote control, compact structure, no electromagnetic interference and high safety, and are now widely used to study the interaction between biomolecules , an important research tool for gene expression, new drug development, environmental testing, food safety and nuclear radiation monitoring. [0003] There are two kinds of bio-optical sensing methods: one is to use the change principle of the refractive index of biomolecules, and its main feature is that biomolecules do not need to be labeled, and the sample preparation is simple; the other is to use the change principle of biomolecular fluorescent la...

Claims

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Application Information

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IPC IPC(8): G01N21/41
Inventor 贾振红李鹏吕小毅
Owner XINJIANG UNIVERSITY