Beta-amylase-trehalose synthase fused enzyme and expression gene and application thereof

A technology of trehalose synthase and gene expression, which is applied in the field of genetic engineering, can solve the problems of difficult separation of maltose and trehalose, difficult production conditions, and increased difficulty of separation and purification, so as to facilitate the control of process conditions and simplify the production process , Promote the effect of continuing to decompose starch
CN104059902AActive Publication Date: 2014-09-24QILU UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
QILU UNIV OF TECH
Publication Date
2014-09-24

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Abstract

The invention relates to a beta-amylase-trehalose synthase fused enzyme and an expression gene and application thereof. The amino acid sequence of the beta-amylase-trehalose synthase fused enzyme is as shown in SEQ ID NO.1; the nucleotide sequence of the expression gene of the beta-amylase-trehalose synthase fused enzyme is as shown in SEQ ID NO.2. According to the invention, beta-amylase and trehalose synthase are fused for the first time, and the obtained beta-amylase-trehalose synthase fused enzyme can be used for producing trehalose by utilizing amylose or straight-chain dextrin, and moreover the influence of substrate maltose on the separation of the product trehalose is reduced when the trehalose synthase acts on maltose to generate trehalose.
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Description

technical field

[0001] The present invention relates to β-amylase-trehalose synthase fusion enzyme and its expression gene and application, in particular to a β-amylase-trehalose synthase fusion enzyme which can produce trehalose by using amylose or amylose dextrin The expression gene and application thereof and the expression gene and application thereof belong to the technical field of genetic engineering. Background technique

[0002] Trehalose (Trehalose, α-D-glucopyranosyl-α-D-glucopyranoside) is also known as sucrose and muscarinose, and its chemical name is α-D-glucopyranosyl-α-D-glucopyranoside. Trehalose was first isolated by researchers in Selaginell lepidophylla and subsequently isolated from the ergot fungus of rye in 1982. Trehalose is composed of two glucose molecules connected by α, α-1,1-glucosidic bonds, the reduced part of each glucose molecule is used to form α, α-1,1-glycosidic bonds, and there is no free aldehyde group, Therefore, trehalose is a non-re...

Claims

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