High-strength supramolecular hydrogel, as well as preparation method and application thereof

A supramolecular hydrogel, high-strength technology, applied in the field of biomedical materials, can solve the problems of difficult to meet the long-term sustained release of gene drugs, rapid degradation, etc., to increase degradability, maintain biological activity, and the preparation method is simple and fast. Effect

Inactive Publication Date: 2014-10-01
广州新犇富科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the gene transfection efficiency of this material has been improved, its degradation is too fast to meet the purpose of long-term sustained release of gene medicine

Method used

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  • High-strength supramolecular hydrogel, as well as preparation method and application thereof
  • High-strength supramolecular hydrogel, as well as preparation method and application thereof
  • High-strength supramolecular hydrogel, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of high-strength supramolecular hydrogel, comprising the following steps:

[0046] 1. Drying of polyethylene glycol: Dry the polyethylene glycol under vacuum at 60° C. for 24 hours.

[0047] 2. Drying of the caprolactone monomer: Distill the caprolactone monomer under reduced pressure at 90°C.

[0048] 3. Drying of dichloromethane and pyridine: Add calcium hydride powder into dichloromethane or pyridine solution (add 1-2 grams of calcium hydride per 500ml solution), stir at room temperature for 24 hours and then distill.

[0049] 4. Synthesis of polyethylene glycol-polycaprolactone-polycationic copolymer (corresponding to "step 1)" in the claims);

[0050] a. Synthesis of polyethylene glycol-polycaprolactone block polymer:

[0051] Add 15 grams of dry polyethylene glycol (weight average molecular weight 1900), 10 grams of caprolactone monomer, and 0.05 grams of stannous octoate into the reaction device at the same time, and use an oil pump to evac...

Embodiment 2

[0061] A preparation method of high-strength supramolecular hydrogel, comprising the following steps:

[0062] 1. Drying of polyethylene glycol: Dry the polyethylene glycol under vacuum at 60° C. for 24 hours.

[0063] 2. Drying of the caprolactone monomer: Distill the caprolactone monomer under reduced pressure at 90°C.

[0064] 3. Drying of dichloromethane and pyridine: Add calcium hydride powder into dichloromethane or pyridine solution (add 1-2 grams of calcium hydride per 500ml solution), stir at room temperature for 24 hours and then distill.

[0065] 4. Synthesis of polyethylene glycol-polycaprolactone-polycationic copolymer (corresponding to "step 1)" in the claims);

[0066] a. Synthesis of polyethylene glycol-polycaprolactone block polymer:

[0067] Add 15 grams of dry polyethylene glycol (weight average molecular weight 1900), 5 grams of caprolactone monomer, and 0.05 grams of stannous octoate into the reaction device at the same time, and use an oil pump to evacu...

Embodiment 3

[0077] A preparation method of high-strength supramolecular hydrogel, comprising the following steps:

[0078] 1. Drying of polyethylene glycol: Dry the polyethylene glycol under vacuum at 60° C. for 24 hours.

[0079] 2. Drying of the caprolactone monomer: Distill the caprolactone monomer under reduced pressure at 90°C.

[0080] 3. Drying of dichloromethane and pyridine: Add calcium hydride powder into dichloromethane or pyridine solution (add 1-2 grams of calcium hydride per 500ml solution), stir at room temperature for 24 hours and then distill.

[0081] 4. Synthesis of polyethylene glycol-polycaprolactone-polycationic copolymer (corresponding to "step 1)" in the claims);

[0082] a. Synthesis of polyethylene glycol-polycaprolactone block polymer:

[0083] Add 15 grams of dry polyethylene glycol (weight average molecular weight 5000), 10 grams of caprolactone monomer, and 0.05 grams of stannous octoate into the reaction device at the same time, and use an oil pump to evac...

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Abstract

The invention relates to a method for preparing high-strength supramolecular hydrogel. The method comprises the following steps: (1) synthesizing polyethylene glycol-polycaprolactone-polycationic copolymer; (2) preparing a copolymer/nucleic acid composite liquid; and (3) mixing the copolymer/nucleic acid composite liquid obtained in the step (2) and an alpha-cyclodextrin liquid to prepare the high-strength supramolecular hydrogel. The invention also relates to the high-strength supramolecular hydrogel prepared by the method and an application of the hydrogel. The hydrogel forming process does not have chemical crosslinking reactions, the influence of chemical crosslinking reaction by-products on the activity of nucleic acid can be effectively avoided, the hydrogel has an effective gene transfer effect, the preparation method is convenient and quick, the hydrogel can be formed at room temperature, and the bioactivity of an embedded gene and the biocompatibility of a material can be kept. According to the hydrogel, the defect of quick degradation of current similar gel materials can be overcome, the nucleic acid can be released for more than 2 weeks, and the aim of long-time durable transfection can be achieved.

Description

[0001] technical field [0002] The invention relates to the field of biomedical materials, in particular to a high-strength supramolecular hydrogel that can be used as a gene carrier and its preparation method and application. Background technique [0003] Gene therapy refers to the introduction of exogenous genes into cells to correct or compensate for diseases caused by gene defects and abnormalities to achieve therapeutic purposes. It is currently an effective method for the treatment of cancer and congenital immune system diseases. The key to the implementation of this technology is to select the appropriate gene carrier and gene introduction method, so that the gene can be expressed in cells safely, efficiently, controllably and stably. As a class of biomedical materials that can be used as drug or gene carriers, hydrogel has the characteristics of high loading rate, protection of macromolecular drugs from degradation or removal by organisms, and sustainable release of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K47/34C08J3/075C08G81/00C08G63/91C08G63/664A61P35/00A61P37/02
Inventor 张宏斌武婕隆湘蕾
Owner 广州新犇富科技有限公司
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