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Method for postprocessing of hericium erinaceus fermentation mycelium solution and preparing mycelium powder through biological enzyme

A technology of Hericium erinaceus and Hericium erinaceus, which is applied in the field of health food preparation, can solve the problems of unstable final product quality, difficult separation and preparation, long fermentation cycle, etc. The effect of large-scale production and short production cycle

Inactive Publication Date: 2014-10-08
乳山市华隆生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention solves the problem that the existing Hericium erinaceus mycelium has a long solid fermentation cycle, easy to infect bacteria, and the quality of the final product is unstable, and the existing liquid fermented Hericium erinaceus mycelium liquid is difficult to further separate due to the large number of mycelium fibers. Preparation of mycelium powder and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] 1. Prepare culture medium

[0032] A: Preparation of slant medium

[0033] According to the proportion of 18% potatoes by mass, 2% glucose, 2% agar, and 1000ml water, the pH is natural, and it is divided into test tubes. After mixing, it is sterilized at high temperature. Sterilization conditions: 121~123°C, 30~40min; Take out the test tube 10-15 minutes after the end of sterilization, place it on a slope, transfer the culture medium on the slope to a 28-36°C incubator for 18-20 hours at a constant temperature, and take it out for use if no colonies grow out;

[0034] B: Preparation of Shake Flask Medium

[0035] According to the proportion of 18% potatoes by mass, 2% glucose, and 1000ml water, the pH is natural. After mixing, it is sterilized at high temperature. Sterilization conditions: 121~123℃, 30~40min, after cooling, put it in a sterile inoculation room for inoculation use;

[0036] C: Preparation of Seed Pot Medium

[0037] Weigh ...

example 2

[0055] 1. Prepare culture medium

[0056] A: Preparation of slant medium

[0057] According to the mass percentage of 19% potatoes, 2.5% glucose, 2.5% agar, and 1000ml water, the pH is natural, divided into test tubes, mixed and then sterilized at high temperature. Sterilization conditions: 121~123°C, 30~40min; Take out the test tube 10-15 minutes after the end of sterilization, place it on a slope, transfer the culture medium on the slope to a 28-36°C incubator for 18-20 hours at a constant temperature, and take it out for use if no colonies grow out;

[0058] B: Preparation of Shake Flask Medium

[0059] According to the proportion of 19% potatoes by mass, 2.5% glucose, and 1000ml water, the pH is natural. After mixing, it is sterilized at high temperature. Sterilization conditions: 121~123°C, 30~40min, after cooling, put it in a sterile inoculation room for inoculation use;

[0060] C: Preparation of Seed Pot Medium

[0061] Weigh 6kg of glucose,...

example 3

[0079] 1. Prepare culture medium

[0080] A: Preparation of slant medium

[0081] According to the proportion of 20% potatoes by mass, 3% glucose, 3% agar, and 1000ml water, the pH is natural, and it is divided into test tubes. After mixing, it is sterilized at high temperature. Sterilization conditions: 121~123°C, 30~40min; Take out the test tube 10-15 minutes after the end of sterilization, place it on a slope, transfer the culture medium on the slope to a 28-36°C incubator for 18-20 hours at a constant temperature, and take it out for use if no colonies grow out;

[0082] B: Preparation of Shake Flask Medium

[0083] According to the proportion of 20% potatoes by mass, 3% glucose, and 1000ml water, the pH is natural. After mixing, it is sterilized at high temperature. Sterilization conditions: 121~123°C, 30~40min, after cooling, put it in a sterile inoculation room for inoculation use;

[0084] C: Preparation of Seed Pot Medium

[0085] Wei...

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Abstract

The invention provides a method for postprocessing of hericium erinaceus fermentation mycelium solution and preparing mycelium powder through biological enzyme. The method solves the problems that according to existing hericium erinaceus mycelium, the fermentation period is long, contamination is prone to appearing, and the final product quality is not stable and also solves the problems that the number of mycelium fibers in an existing hericium erinaceus fermentation mycelium solution is large, the fibers cannot be easily separated, and further preparation of the mycelium powder is limited. The method comprises the steps of preparing a culture medium, activating a culture, inoculating, performing cultivating, expanding fermentation, performing enzymolysis, performing concentrating, performing homogenizing, performing spraying and performing drying. The method for preparing the culture medium comprises the steps of preparing an inclined plane culture medium, preparing a shake flask culture medium, preparing a seeding tank culture medium, and preparing a fermentation tank culture medium. The method is widely applied to processing the hericium erinaceus mycelium powder in the food industry.

Description

technical field [0001] The invention relates to a method for preparing health food, in particular to a method for post-processing Hericium erinaceus fermented mycelium liquid and preparing mycelium powder with the assistance of biological enzymes. Background technique [0002] Hericium erinaceus ( Hericium erinaceus ) is also known as Hericium erinaceus. Drug efficacy studies have shown that it contains polymer compounds such as polypeptides, polysaccharides and amides, which can improve the body's ability to resist hypoxia, increase cardiac output, accelerate blood circulation, reduce blood sugar and blood pressure, protect the liver, and protect the liver. It can enhance the body's immune function and improve immunity by enhancing the phagocytosis of peritoneal macrophages. In addition, it also has a better protective effect on gastric mucosa. However, in the food industry, fruiting bodies or mycelia are obtained through solid culture, and the culture time needs a cer...

Claims

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Application Information

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IPC IPC(8): A01G1/04A23L1/28A23L1/29A23L33/00
Inventor 张文镨王溢刘天伦
Owner 乳山市华隆生物科技股份有限公司
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