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Method for rapidly sensitively detecting sodium valproate in blood

A sodium valproate and sensitive technology, applied in the field of detection of sodium valproate in blood, can solve the problems of difficulty in development in primary medical institutions and high cost of fluorescence polarization immunoassay analyzers, to ensure clinical safety, fast measurement speed, and measurement The effect of simple method

Inactive Publication Date: 2014-10-22
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Fluorescence polarization immunoassay, fluorescence polarization immunoassay mainly relies on the TDx-FLx fluorescence polarization immunoassay analyzer, after taking the serum, use Abbott’s sodium valproate kit to complete the determination of blood drug concentration easily and quickly, but the TDxFLx fluorescence polarization immunoassay The cost of polarization immunoanalyzer is high, and it is difficult for primary medical institutions to carry out

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  • Method for rapidly sensitively detecting sodium valproate in blood
  • Method for rapidly sensitively detecting sodium valproate in blood
  • Method for rapidly sensitively detecting sodium valproate in blood

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Experimental program
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Effect test

Embodiment 1

[0034] figure 2 In the positive ion mode, the ion peak of the air reagent irradiated by the halogen lamp appears at about 7.8ms;

[0035] Dissolve sodium valproate in methanol, prepare 1000 mg / L sodium valproate standard, take 10 μL of the standard and drop it on the sample sheet, use the ion mobility spectrometer to detect and analyze in the positive ion high pressure mode, thermal analysis After the sample, measure the migration time and signal peak intensity of ions reaching the ion mobility spectrometer detector;

[0036] image 3 It is the ion mobility spectrogram of detecting 10 μL, 1000ppm sodium valproate standard in positive ion mode, and the detection signal of sodium valproate is at about 11.6ms. This detection signal is used for calibration with the detection signal of sodium valproate in blood.

Embodiment 2

[0038] Take 10-100μL blood sample with a micro-sampler at the sampling place of the sample injection slice, and irradiate the halogen lamp for 8S. The detection signal of the blood sample is about 9ms and 11.8ms, such as Figure 4 Shown; Compared with the follow-up results, it can be seen that the endogenous substances in the blank blood do not interfere with the detection of sodium valproate epilepsy drug.

Embodiment 3

[0040] The detection signal of sodium valproate (100ppm) in blood is as follows: Figure 5 shown. The detection signal of sodium valproate (100ppm) in blood is about 11.64ms, and the signal intensity is about 100mv.

[0041] Quantitative analysis test, according to the signal intensity of sodium valproate in different concentrations of blood, can establish a quantitative analysis marking line equation within the concentration range of the doctor's administration, and analyze the blood drug concentration of the patient after administration. Quantitative analysis concentration range is 5-500mg / L. The established qualitative and quantitative analysis methods meet the concentration analysis range (50-100mg / L) that doctors give to epilepsy patients.

[0042] The device in the invention is stable and reliable, and the method is simple, fast and efficient. The detection and analysis time of a single sample is less than 20S. The detection sensitivity is high, and the measurement d...

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Abstract

The invention discloses a method for rapidly sensitively detecting sodium valproate in blood. An ion migration spectrum technology is taken as a basic detection technology, an ion migration spectrometer is utilized as a detection means, a positive-ion high-voltage mode is employed, a halogen-lamp thermal desorption sampling manner is employed, and the method does not comprise any solvent extraction pretreatment. An apparatus employed in the method is stable and reliable, and the method is simple, rapid and efficient. The detection analysis time of a single sample is less than 20 S. The detection sensitivity is high, the measure detection limit can reach 1 mg / L, and the quantitative analysis concentration scope is 5-500 mg / L. The established qualitative quantitative analysis method satisfies the human-body medicine-administration concentration analysis scope (50-100 mg / L) of a doctor to an epilepsy patient. The detection method is widely applicable to clinical medicine-administration deep analysis, and helps to guide a doctor to clinically precise administrate a medicine.

Description

technical field [0001] The invention discloses a fast and sensitive method for detecting sodium valproate in blood. The method uses ion mobility spectrometry as the basic detection technology, uses ion mobility spectrometer as the detection means, adopts negative ion high pressure mode, halogen lamp thermal analysis sample injection mode, and does not have any solvent extraction pretreatment method. The device in the invention is stable and reliable, and the method is simple, fast and efficient. The established qualitative and quantitative analysis methods meet the concentration analysis range (50-100mg / L) that doctors give to epilepsy patients. The detection method in the present invention can be widely used in the in-depth analysis of clinical drug administration, and guides clinicians in precise drug use. Background technique [0002] Sodium valproate is an antiepileptic drug, and its mechanism of action has not been fully elucidated. Experiments show that this product...

Claims

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Application Information

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IPC IPC(8): G01N27/64
Inventor 王新李海洋王祯鑫渠团帅
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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