The application discloses a
hyperspectral imaging device and method based on Fourier superposition
microscopy of spectral
multiplexing, and relates to the field of
microscopic imaging. The device is composed of a
halogen lamp, a
beam splitter, a filter, a total reflection
prism, a beam combiner, a reflector, a sample to be measured, an x-y plane plate, a microscopic objective, a
pupil aperture, a sleeve objective and a
CMOS camera. The spectral
multiplexing illumination device is coupled with light of different wave bands as a
light source to irradiate the sample, and a series of incoherent mixed state low-resolution intensity images are acquired by the
CMOS camera. The above images are decoupled and recovered based on a phase
recovery Fourier superposition
algorithm of spectral
multiplexing, a series of discrete spectral images are acquired, an interpolation
algorithm is used to interpolate the series of discrete spectral images, and an image with a
large field of view, high spatial and high
spectral resolution is reconstructed. The device provided by the application can couple multiple beams of known
wavelength light, and the low-resolution image captured by the
CMOS camera contains spectral information of multiple accurate wavelengths.