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Method for electrochemical detection of human immune globulin E (hIgE)on the basis of magnetic Fe3O4 @ Au nano composite material

A technology of composite nanomaterials and material electrochemistry, which is applied in the direction of material inspection products, analytical materials, measuring devices, etc., can solve the problems of complex operation, radiation, and low sensitivity, so as to achieve the safety of the detection process, shorten the detection time, and reduce the particle size. evenly distributed effect

Active Publication Date: 2014-11-05
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

Among them, radioimmunoassay has radiation and is not safe; other detection methods are complicated to operate and have low sensitivity, which makes it difficult to realize early diagnosis and research of diseases, and cannot meet the needs of rapid detection.

Method used

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  • Method for electrochemical detection of human immune globulin E (hIgE)on the basis of magnetic Fe3O4 @ Au nano composite material
  • Method for electrochemical detection of human immune globulin E (hIgE)on the basis of magnetic Fe3O4 @ Au nano composite material
  • Method for electrochemical detection of human immune globulin E (hIgE)on the basis of magnetic Fe3O4 @ Au nano composite material

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Embodiment Construction

[0041] The steps of the present invention to detect hIgE are as follows:

[0042] 1. Magnetic Fe 3 o 4 Preparation of nanomaterials: weigh 2.50 g FeSO 4 ·7H 2 Dissolve O in 30 mL of pure water, add 10 mL of 50 g / L polyethylene glycol-20000 (PEG-20000) solution, and stir evenly; add 30 mL of dilute ammonia solution drop by drop under constant stirring, and continue to add 270 μL 30% H 2 o 2 The solution was transferred to an autoclave, reacted at a constant temperature of 160 °C for 6 h, magnetically separated and washed 3 times, and dried at 70 °C to obtain Fe 3 o 4 Nanoparticles;

[0043] 2. Fe 3 o 4 Preparation of Au composite nanomaterials: weigh 10 mg Fe 3 o 4 Nanoparticles were uniformly dispersed by ultrasonication with 120 mL 50 g / L PEG-20000 solution; 1 mL 1.0% chloroauric acid solution was added under stirring conditions, and reacted for 1 h; after adding 5 mL 80 mmol / L hydroxylamine hydrochloride solution, the reaction was continued for 1 h; sequentially ...

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Abstract

A method for electrochemical detection of human immune globulin E (hIgE) on the basis of magnetic Fe3O4 @ Au nano composite material is as follows: Fe3O4 @ Au nano composite material is prepared by one-step reduction method, Fe3O4 @ Au nano composite material is labeled with hIgE antibody; the Fe3O4 @ Au nano composite material is mixed with the hIgE and a biotinylated hIgE aptamer to form a hIgE antibody-hIgE-hIgE aptamer complex; avidin alkaline phosphatase is adsorbed to the hIgE antibody-hIgE-hIgE aptamer complex, and silver ions are catalytically reduced on the surface of the magnetic Fe3O4 @ Au composite nano material into elemental silver and deposited onto the surface of the nano composite material by biological catalysis deposition reaction of the alkaline phosphatase. By detection of dissolving-out voltammetric current value of the elemental silver, the detection of the hIgE can be achieved. The magnetic Fe3O4 @ Au nano composite material has the particle size of 35-45nm, and has the advantages of uniform particle size distribution and superparamagnetism.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for electrochemically detecting human immunoglobulin E based on a magnetic composite nanometer material. Background technique [0002] Human immunoglobulin E (human immunoglobμLin E, hIg E), is a kind of allotropic antibody with a σ chain, and is the main antibody involved in the regulation of the pathogenesis of allergic rhinitis, allergic asthma and eczema. One of the main initiating factors. At present, human serum IgE level is an important indicator for in vitro diagnosis of allergic diseases. The detection methods mainly include radioallergen adsorption test, CAP allergen detection method, radioimmunoassay, enzyme-linked immunoassay, fluorescence immunoassay, etc. Among them, the radioimmunoassay method has radiation and is not safe; other detection methods are complicated to operate and have low sensitivity, making it difficult to realize...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/54326G01N33/54346G01N33/54373G01N33/6854
Inventor 黄勇黄国银李桂银周治德梁晋涛马龙飞管明源
Owner GUILIN UNIV OF ELECTRONIC TECH
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